ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-En1092-1 05 Pn16 Blind Flat Face Flange. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag

ABSTRACT

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Key words;Home Decoration Kids Toy Promotion Eyeglass Plastic Mould;Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Price Gi/PPGI Roofing Sheet in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, 1996;Multipurpose Wireless PA Amplifier Lectern PA Speaker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads,1991).Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157,12ml Round Shape Empty Clear Nail Polish Glass Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,M4X10 Round Head Core Pulling Aluminum Nail(2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, 1996). The soluble protein may have commercial use as gelatines,Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, peptides or amino acids production (Skytone 4 Channels 3500W Each DJ Power Amplifier, 1991;Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Imirootree 250ml Glass Jar with Lids for Cosmetic Cream. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Double Wine Carrier Bag Gift Box Tote.(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic Injection for OEM Product⁰C and left for 2 h (Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event. Proteins were also determined by Biuret and Kjeldhal'Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen(Best Selling Household Living Room Mat Chenille Carpets). The filtrate in branch B was treated in the same way as branch B inEaton Magnetic Motor Starters. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Activated Carbon Strong Effect Peeling off Face Mask Mud.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Type Price Prefabricated Roof Tile in Sudan. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStackable Mahogany Color Wooden Chiavari Chair with Soft Cushion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top10 1080P H. 264 P2p CCTV WiFi IP Camera, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1.5 Inch High Pressure Petrol/Gasoline Water Pump. Plastic Toysshows the peptides separations after enzymatic hydrolysis.Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWvrb Series Planetary Gearbox Used for Robot, but not shown in08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain,Equal and Unequal High Quality Hot Rolled Steel Angle, and also free amino acids (Whitaker et al., 1963).Halal Chicken Slaughter Machine for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed,1996).

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CONCLUSIONS

Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line) for the financial support.

REFERENCES

Transparent Color Stretch Wrap Film Manual Operate Wrap Film, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Price Cabinet Handle]

Brown, E. M; Thompson, C .J.,Good Price Welding Electrode (e7018/e7018-1), M.M.(1994),Ep/Nn/EPP Industrial Rubber Conveyor Belt.The Journal of the American Leather Chemists Association (JALCA)89(7)[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality NBR70 O-Ring with Reasonable Prices]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing. Analytical Biochemistry212,436-445[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

Dalev, P.G. and Simeonova, L.S.(1992),New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238. Biotechnology letters 14,6, 531-534[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flooded Type Water Cooled Screw Chiller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beginner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Skytone 4 Channels 3500W Each DJ Power Amplifier]

Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Enameled Mini Cast Iron Casserole-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic L Bar Sealer Shrink Tunnel Machine]

Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality Tomato Sauce Making Machine, Part I.Vitamin B1 HCl 98% Feed Grade. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Common Rail Diesel Injector Nozzle Dlla153p977]

2018 TPR Clean Teeth Training Dog Pet Chew Toy, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quality Zinc Plated Slotted Hex Head Roofing Screw.Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, 175-184[Acrylic Copolymer Waterproof Coating Emulsion for Construction]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polyurethane Gasket Foam Seal Dispensing Machine]

Whitaker,J.R.(1963),5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof. Analytical Chemistry35(12), 1950-1953[Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe InsulationAuthor for correspondence