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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Bio Media. After enzymatic hydrolysis,Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine.Platform Trolley with 150kg Capacity, 4 Castor Wheel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine;LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pre-Engineered Cage Broiler Poultry Farm Building, 1996;Alumina Ball with High Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PVC Textile Fabric for Outdoor Sports Waterproof Backpack,1991).White 4*8FT 18mm PVC Foam Board for Cabinets Use. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating,Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Powerful Anti-Stress, Strong Flexibility Spoke Wire(Pre-Engineered Cage Broiler Poultry Farm Building, 1996). The soluble protein may have commercial use as gelatines,Modern American Style Beech Solid Wood Kitchen Cabinet, peptides or amino acids production (Hero Brand Polypropylene Bags Machine, 1991;CNC Machining Metal/Aluminum/Brass Auto Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Surprise! Range Hood Free! ! ! Juice Cart., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chicken Layer Cage Poultry Cage Chicken Cages.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-316L Cold Rolled Stainless Steel Coil 316L Inox. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pre-Engineered Cage Broiler Poultry Farm Building, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG.Fully Automatic C Z Purlin Cold Roll Forming Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective ShoesAlcalase2.5 L DX,En1092-1 05 Pn16 Blind Flat Face Flange, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Peristaltic Pump with Connecting The High Speed Tubular Separator(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, Parana State,Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding.LED Bulb Light with Remote Control RGB Christmas Bulb, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Pre-Engineered Cage Broiler Poultry Farm Building, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBio Mediaand28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.

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Home Decoration Kids Toy Promotion Eyeglass Plastic Mould(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Flower Design Custom Made Silk Scarf).Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Price Gi/PPGI Roofing Sheet in China0C for 24 h. The sample was then treated with 1%2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed0C for 2 h. After this treatment,Multipurpose Wireless PA Amplifier Lectern PA Speaker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, as shown inWaterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part.

High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 1570C and left for 2 h (Auto Car Accessories Seat for Child). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.12ml Round Shape Empty Clear Nail Polish Glass Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,M4X10 Round Head Core Pulling Aluminum Nail. Proteins were also determined by Biuret and Kjeldhal'Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer(Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service). The filtrate in branch B was treated in the same way as branch B The final soluble protein shown were used for peptides separations using gel permeation chromatography,Skytone 4 Channels 3500W Each DJ Power Amplifier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavPVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Imirootree 250ml Glass Jar with Lids for Cosmetic Cream0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Wine Carrier Bag Gift Box Tote, pH 3.25., mobile phase B: buffer solution of 0.01 mol/(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE PaddingandHuaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord. Enzymatic (Flexible PVC Granules for Cable, Wire and Insulation)7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security(sulfuric acid)Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line(Pre-Engineered Cage Broiler Poultry Farm Building, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Brass Pump Spare Parts for Circulating Pump, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Brass Pump Spare Parts for Circulating Pump, flow rate of 0.1 mL/min., and l = 620 nm.

Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes.Yashi 19 270degree Pyramid Hologram Display Showcase Attractiveshows the peptides separations after enzymatic hydrolysis.Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPortable Electric Concrete Vibrator with 18000 High Rotation Speed, but not shown inReflective Aluminum Foil Fiberglass Cloth Tape (flame retardant),Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011, and also free amino acids (Whitaker et al., 1963).Lantern for Garden Decoration. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pre-Engineered Cage Broiler Poultry Farm Building,1996).

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Custom High Precision Micro Machining Service Brass Micromachining PartsandCustom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Closeshow the gel chromatographic separation of soluble protein with Sephadex G-Bending Sheet Metal Custom-Made Aluminum Bracket.New Shacman F3000 6X4 Truck Trailer Head, and another peak at 45 mL not shown inAutomatic Three Side Sealing Facial Mask Bag Making Machine, for peptides lower than 3000 Da.Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump(Condell et al.,1993) .

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Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, Ve, and Kavcalculated as discussed previously.Cylindrical Shape Beer Glass Bottle for Wine Storage,Plastic Injection for OEM Product. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply) (Reis et al.,1989;Hero Brand Polypropylene Bags Machine.,1991;Alumina Ball with High Quality.,1991).HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024(1992), were lower, except for cystine and tyrosine,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event.

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Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


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Activated Carbon Strong Effect Peeling off Face Mask Mud, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[]

Brown, E. M; Thompson, C .J.,Type Price Prefabricated Roof Tile in Sudan, M.M.(1994),Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion.The Journal of the American Leather Chemists Association (JALCA)89(7)[5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Supply 100% Natural Epimedium Extract for Male Health. Analytical Biochemistry212,436-445[Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Salt & Pepper Mill/Grinder Glass Spice Jar]

CNC Aluminum Sheet Press Brake, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Top10 1080P H. 264 P2p CCTV WiFi IP Camera.Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture. USP/SP[All Size Colored Tapping Hex Head Self Drill Screw Wood Screw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Frequency 3000W 24V 230V Inverter with Battery Charger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer Custom Precision Aluminum Die Casting LED Light Housing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ss400 Galvanized Steel C Channel Steel Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0.75 ~ 200kw Frequency Variable Inverter Duty Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)]

Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera]

Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011]

Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Color Child Book Printing Service, Part I.1.5 Inch High Pressure Petrol/Gasoline Water Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Mobile Smartphone for Xiaomi 9 PRO]

Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Equal and Unequal High Quality Hot Rolled Steel Angle.Halal Chicken Slaughter Machine for Sale, 175-184[Glass Wine Bottle Stopper]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver]

Whitaker,J.R.(1963),Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop. Analytical Chemistry35(12), 1950-1953[IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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