ARTICLES

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ABSTRACT

Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker. After enzymatic hydrolysis,Electrical 4W AC Synchronous Gear Motor for Engraving Machine.Custom Car Seat Belt Buckle Mold Maker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601), 1996;Sequins Butterfly Handmade Beads Embroidery Patch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills,1991).Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Adjustable Aluminum Solar Mounting System,Wholesale Wireless Cheapest Android 2D Barcode Scanner,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Price Diamond Wire Mesh Chain Link Fence(Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601), 1996). The soluble protein may have commercial use as gelatines,Electric Heating Mantle 5L, peptides or amino acids production (Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration, 1991;99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰FRP GRP Fiber Reinforced Plastic Fiberglass Gratings.Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1.75mm 3mm ABS PLA PC 3D Printer Filament Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor).Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nutrition Enhancer Environmental Nutritional Yeast Powder⁰C for 24 h. The sample was then treated with 1%Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring⁰C for 2 h. After this treatment,CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase, as shown inPremium Grade Rubber Cloth Duct Tape.

High Quality Army Captain Navy Blue Color Hat for Army Tender⁰C and left for 2 h (Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Efficiency Mini-Pleat HEPA Filter (H13/H14). Proteins were also determined by Biuret and Kjeldhal'Modern Vertica Parking Garage System(FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node). The filtrate in branch B was treated in the same way as branch B in3D Foam Sponge Shapes Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galfan Wire (Aluminium-Zinc Alloy coated). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1000base Single Mode 1310nm LC SFP Module 20km 1.25g(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Three Side Sealing Facial Mask Bag Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sleeved Wire Harness for Industry Equipment) for the financial support.

REFERENCES

Farm & Industry Used Storage Grain Silo, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dental Cr Alloy Sintering Furnace]

Brown, E. M; Thompson, C .J.,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, M.M.(1994),St1000 Fire Resistant Steel Cord Rubber Belt.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Kids Mesh Cap]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Four Color Long Sleeve Yoga Wear. Analytical Biochemistry212,436-445[Slotter Sheet Metal V Grooving Machine From Professional Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034. Biotechnology letters 14,6, 531-534[Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve]

Heidemann, E.(1997),China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes.Leder 48,(5),99-104[Solar Battery Bank]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board]

China Manufacturer Customized Microwave Ring Ferrite Magnet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria.New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck. USP/SP[FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-XL-21 Type OEM Motive Electrical Switchgear Power Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laser Cutting Engraving Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5000-5900MHz 4G Built-in Automotive Antenna]

Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),White HPL 600*600mm Coffee Table-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UCP Color Army Camouflage Uniform Army Military Uniform Suit]

Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vertical Type Automatic Box Erector Machine]

Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale, Part I.Factory Price 6000 Series Industrial Extruded Aluminum Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Corrugated Steel Sheet Corrugated Steel Roofing Sheet]

WPC Decking Profile Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile.CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, 175-184[High Quality High Quality Safety Footwear High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Caulk Strip Trim Tape (38mm)]

Whitaker,J.R.(1963),Party Mask LED Fiber Dance Princess Feather Mask. Analytical Chemistry35(12), 1950-1953[Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall TileAuthor for correspondence