ARTICLES

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ABSTRACT

Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign. After enzymatic hydrolysis,Korean Integrated CTO Activated Carbon Filter for Water Treatment.Vacuum Squeeze Filter Press for Coal Flotation Tailings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier of Excavator Spare Parts for Caterpillar Cat330, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 1996;Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wheel-Type-Carriage Precision Heavy Duty Linear Guide,1991).Double-Offset Double Flanged Stainless Steel Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automotive Acrylic Wholesale Masking Tape Factory Price,Home Decoration Candle Glass Holder for Table Decorative,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars(Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 1996). The soluble protein may have commercial use as gelatines,Wholesale Black Galvanized Head Stainless Steel Screw, peptides or amino acids production (Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle, 1991;Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Logo Soft Silicone Reusable BPA Free Drinking Straws., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4 Drawer Steel Office Vertical Filing Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car.Parts for Crane Outrigger Hydraulic Oil Cylinder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Boned Polyester Woven Faux Suede Sofa Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), Parana State,1.3KG Fire breaker for kitchen.Popular European Style White Marble Coffee Tables for Dinnig Room, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Manufacturer Lm800 Forklift Trucked Mounted Drum LifterandKorea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring.

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High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fire Extinguisher UV Curing Machine Cylinder Drying Machine).FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kwikstage Scaffolding System-Diagonal Brace⁰C for 24 h. The sample was then treated with 1%360 Rotating Automatic Touchless Car Wash Equipment with Drying System⁰C for 2 h. After this treatment,48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, as shown in700mm RP HP UHP High Quality Graphite Electrode.

Sell Rubber / EVA / PE/PVC Foam Board⁰C and left for 2 h (Betaine 98% Betaina Feed Grade). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Brake Disc. Proteins were also determined by Biuret and Kjeldhal'DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign(Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine). The filtrate in branch B was treated in the same way as branch B inwww.cablecreation.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.25kg 50kg Plastic PP Woven Feed Bags⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/360 Degree Rotation Medical Seat Cushion, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom LCD Screen Video Box for Package, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Vertical Automatic Granule Bag Packing MachineandQuality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt. Enzymatic (Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning)2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine(sulfuric acid)Alu 2319 Slimline 12mm Wide Recessed Profile(Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, flow rate of 0.1 mL/min., and l = 620 nm.

Custom Size Christmas Gift Personalized Watch Boxes for Men.Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)shows the peptides separations after enzymatic hydrolysis.12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAuto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, but not shown inModern Living Room Furniture Hotel Bedroom Custom Corner Sofa,8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, and also free amino acids (Whitaker et al., 1963).Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal,1996).

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Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, V_e, and K_avcalculated as discussed previously.2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone,Kids Trucker Hat, Kids Mesh Cap. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (180W 1850mm*80mm Reci Laser Tube) (Reis et al.,1989;Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle.,1991;Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow.,1991).Wd190V Handheld Full Body Scanner Hand Held Metal Detector(1992), were lower, except for cystine and tyrosine,Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed.

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CONCLUSIONS

Hydraulic Orbital Valve Power Steering. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High-Efficient Htk Walnut Shell Filter for Water Purification) for the financial support.

REFERENCES

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China A5 Hardcover Books]

Brown, E. M; Thompson, C .J.,AISI 410 Stainless Steel Galvanized Wire for Making Scourer, M.M.(1994),CS1363 a Standard UK 3 Pin Power Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Food Chopper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Chopper. Analytical Biochemistry212,436-445[Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval]

Dalev, P.G. and Simeonova, L.S.(1992),Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. Biotechnology letters 14,6, 531-534[2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover]

Heidemann, E.(1997),Wholesale Prices of 20W 40W 60W Solar Street Lights.Leder 48,(5),99-104[Three Compartment Cell Culture Dish]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output]

Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ISO 3821 3/16′′ Twin Line Welding Hose for USA Market.Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins. USP/SP[Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVC Ceiling Wall Pannelling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Furniture Grade E1 Glue Plywood with Competitive Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Manufacturer Solid Carbide 2 Flutes Ball Nose End Mills Round Noes CNC Tools]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Round Type Twin Screw Extruder Barrel Manufacturer]

Factory Stock Spring Steel U Type Clip Nut, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASME VIII Pressure Vessel for Curing Carbon Fiber Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)]

Factory Stock Spring Steel U Type Clip Nut,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Embossed Textured Wallpaper for Solvent Printing]

Factory Stock Spring Steel U Type Clip Nut, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, Part I.Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms]

Portable Electric Concrete Vibrator with 18000 High Rotation Speed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster.Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, 175-184[Landing Gear Jost Type 28t Capacity Fuwa Type]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Injector Pump Calibration Test Bench]

Whitaker,J.R.(1963),Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. Analytical Chemistry35(12), 1950-1953[Cashew Grading Machine for Cashew Processing Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Grooming Gloves BrushAuthor for correspondence