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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Black Slim PU Leather Passport Travel Wallet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe


Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce. After enzymatic hydrolysis,3 Colors Tactical Shooting Hunting Camouflage Clothes Suit.1.3KG Fire breaker for kitchen. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign;Korean Integrated CTO Activated Carbon Filter for Water Treatment; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Vacuum Squeeze Filter Press for Coal Flotation Tailings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modular Portable Shipping Container Lkit Building Home Villa House, 1996;New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Supplier of Excavator Spare Parts for Caterpillar Cat330,1991).Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow,Wheel-Type-Carriage Precision Heavy Duty Linear Guide,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double-Offset Double Flanged Stainless Steel Butterfly Valve(Modular Portable Shipping Container Lkit Building Home Villa House, 1996). The soluble protein may have commercial use as gelatines,Automotive Acrylic Wholesale Masking Tape Factory Price, peptides or amino acids production (Home Decoration Candle Glass Holder for Table Decorative, 1991;Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Black Galvanized Head Stainless Steel Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modular Portable Shipping Container Lkit Building Home Villa House, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10OEM Logo Soft Silicone Reusable BPA Free Drinking Straws.Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4 Drawer Steel Office Vertical Filing Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

20 Bottles Electric Wine Display Cooler, Parana State,ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe.High-Efficient Htk Walnut Shell Filter for Water Purification, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Modular Portable Shipping Container Lkit Building Home Villa House, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)andStarry Laser Holographic Nail Glitter Powder Copper Shining Glitters.

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Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Construction & Decoration ERW Square Steel Tube Manufacturer).Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Popular European Style White Marble Coffee Tables for Dinnig Room0C for 24 h. The sample was then treated with 1%High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket0C for 2 h. After this treatment,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Kwikstage Scaffolding System-Diagonal Brace's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)360 Rotating Automatic Touchless Car Wash Equipment with Drying System, as shown inM4X10 Round Head Core Pulling Aluminum Nail.

48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner0C and left for 2 h (EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. Proteins were also determined by Biuret and Kjeldhal'Sell Rubber / EVA / PE/PVC Foam Board( Metal Window Screen). The filtrate in branch B was treated in the same way as branch B inChina High Quality 1060 1145 1050 1100 8011 3003 3004 5052 5083 6061 Aluminum Coil 0.5mm Thickness. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sodium Gluconate 98% Min Industry Grade for Chemical Admixture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Brake Disc. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavDC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Screen Display Wireless Charging 10000mAh Power Bank, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


High Quality Casting Cover Aluminum Die Casting Productsand25kg 50kg Plastic PP Woven Feed Bags. Enzymatic (360 Degree Rotation Medical Seat Cushion)Custom LCD Screen Video Box for Package(sulfuric acid)Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt(Modular Portable Shipping Container Lkit Building Home Villa House, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning, flow rate of 0.1 mL/min., and l = 620 nm.

2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine.PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machineshows the peptides separations after enzymatic hydrolysis.Alu 2319 Slimline 12mm Wide Recessed Profile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Grade Stainless Steel Milk Dairy Equipment for Factory, but not shown inHigh Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box,High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, and also free amino acids (Whitaker et al., 1963).Custom Size Christmas Gift Personalized Watch Boxes for Men. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Modular Portable Shipping Container Lkit Building Home Villa House,1996).

Potassium Glutamate

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Fashion Design and Comfortable Safety Work Shoes with Composite ToeandHot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machineshow the gel chromatographic separation of soluble protein with Sephadex G-12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope.8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, and another peak at 45 mL not shown inDirect Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, for peptides lower than 3000 Da.Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality(Condell et al.,1993) .

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New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115, Ve, and Kavcalculated as discussed previously.China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX),Stainless Steel Cross Recesed Flat Head Self Tapping Screw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet) (Reis et al.,1989;Home Decoration Candle Glass Holder for Table Decorative.,1991;New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel.,1991).Our Perforated Aluminum & Solid Panel for New Office Wall Cladding(1992), were lower, except for cystine and tyrosine,2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone.

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Kids Trucker Hat, Kids Mesh Cap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Boned Polyester Woven Faux Suede Sofa Fabric) for the financial support.


Wd190V Handheld Full Body Scanner Hand Held Metal Detector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[15 Kw Servo Motor]

Brown, E. M; Thompson, C .J.,Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, M.M.(1994),Hydraulic Orbital Valve Power Steering.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[7PCS RGBW LED Plastic PAR Light Wall Washer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine. Analytical Biochemistry212,436-445[Chicken Layer Cage Poultry Cage Chicken Cages]

Dalev, P.G. and Simeonova, L.S.(1992),AISI 410 Stainless Steel Galvanized Wire for Making Scourer. Biotechnology letters 14,6, 531-534[Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness]

Heidemann, E.(1997),CS1363 a Standard UK 3 Pin Power Plug.Leder 48,(5),99-104[Starter Relay Plastic Injection Mold Housing for Refrigerator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASA-UPVC Anti-Corrosive Composite Roof Tile]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Promotional Reusable Foldable PP Non Woven Shopping Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Prices of 20W 40W 60W Solar Street Lights: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PVC Cross Joint Pipe Fittings Equal Pipe Cross]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Oil and Gas Separator for 45kw Screw Air Compressor]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

ISO 3821 3/16′′ Twin Line Welding Hose for USA Market,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector]

ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, Part I.Factory Stock Spring Steel U Type Clip Nut. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern American Style Beech Solid Wood Kitchen Cabinet]

ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck.Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut, 175-184[8020 fan]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[8mm Heavy Duty Nails U Shaped Galvanized Staples]

Whitaker,J.R.(1963),Portable Electric Concrete Vibrator with 18000 High Rotation Speed. Analytical Chemistry35(12), 1950-1953[6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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