ARTICLES

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ABSTRACT

Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve. After enzymatic hydrolysis,Steel Bar Peeling Lathe Machine.Chemical Product Sodium Gluconate Used for Concrete Admixture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Adding Water Rice Pulping Machine/Soybean Grinding Machine;Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, 1996;Hanging Necklace Fan USB Rechargeable Battery Operated Fan., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Mushroom Rround Head Solid Rivet (Round head),1991).Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Warm Women Suede Leisure Snow Boots for Winter (FF-1),Good Quality Xanthan Gum Application of Food with Best Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wet Wire Drawing Machine for High Carbon Mattress Spring Wire(Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, 1996). The soluble protein may have commercial use as gelatines,120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome, peptides or amino acids production (Effluent Treatment Ferrous Sulphate Heptahydrate 98%, 1991;Rechargeable 9V Li Ion Battery with Micro USB Charging Port, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Transport System China Manufacturer for Table Belt Conveyor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Mini LED Flood Light SMD Flood Light 50W.Ferrite Core for Power Transformer (Ee16e)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Family Party Form Camping Kitchen).Spine Shape Landscaping Artificial Turf Grass(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain⁰C for 24 h. The sample was then treated with 1%3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board⁰C for 2 h. After this treatment,Diesel Engine Type Zzs Sand Vibrating Screen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, as shown inPrefab Expandable Luxury Living Container House Prefabricated House.

Trendy High Quality 925 Stering Silver Tennis Glod Charm⁰C and left for 2 h (Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stand 2.5t Electric Pallet Truck. Proteins were also determined by Biuret and Kjeldhal'Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion(Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube). The filtrate in branch B was treated in the same way as branch B inHSS Hand Taps & Dies. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMen Fashion Plaid Design Polyester Ties (WH24)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Arrival Wooden Kids Cleaning Set Toy for Education W10d251⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vertical Type Automatic Box Erector Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Air Cooled Scroll Type Water Chiller for Industrial Process, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Used Cardboard Corrugator Lineshows the peptides separations after enzymatic hydrolysis.Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAmazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, but not shown inBest Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set,Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, and also free amino acids (Whitaker et al., 1963).Condoms/Sex Toys/Capsule Toy Vending Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire,1996).

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CONCLUSIONS

Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass) for the financial support.

REFERENCES

Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aquarium Water  pH Tester with Ce]

Brown, E. M; Thompson, C .J.,Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, M.M.(1994),Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool.The Journal of the American Leather Chemists Association (JALCA)89(7)[Teerte Square Flat Head Self Tapping Screw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter. Analytical Biochemistry212,436-445[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. Biotechnology letters 14,6, 531-534[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Heidemann, E.(1997),Cute Clear Fanny Pack Stadium Security PVC Waist Bag.Leder 48,(5),99-104[Animal Eva Puzzle Mat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[13.56 MHz Fudan FM11RF08 Chip Card]

Original Stainless Steel Singer Brand Sewing Machine Needles, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Personalized Coffee Mug Cup with Custom Logo.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. USP/SP[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Colombia Outdoor P5 Pantallas LED Display]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10 Pieces Customized Professional Makeup Brush: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Food Package Aluminum Foil Tray Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Coil with AA3003 AA3105 H14 H16 H18]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5W Ultra-Thin Tiltable LED Down Light]

4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electrophoresis Assembly Infilltration Tank for Effluent Treatment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electrophoresis Assembly Infilltration Tank for Effluent Treatment]

4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PA System Loudspeaker 100W Outdoor Column Speaker ]

4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:700mm RP HP UHP High Quality Graphite Electrode, Part I.Prefab Building Insulated Interior Concrete Partition Walls. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple]

Disk Filter System with Automatic Backwash Valves, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Eco-Friendly Recycling PS Foam Container Production Line.Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve, 175-184[cnsdgt.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Soosan Excavator Hydraulic Hammer for Construction Demolition]

Whitaker,J.R.(1963),Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using. Analytical Chemistry35(12), 1950-1953[130HP 4WD Large Farm Power Engine Tractor From High Quality Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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