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ABSTRACT

2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. After enzymatic hydrolysis,304 &314 Stainless Steel Seamless Bending Tube.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.7inch Industrial Tablet Computer Naked Resistance Touch Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glass Door Passenger Sightseeing Elevator for Sale, 1996;Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor,1991).Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase,Fcy30 Transport Mini Tipping Dumper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park(Glass Door Passenger Sightseeing Elevator for Sale, 1996). The soluble protein may have commercial use as gelatines,Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), peptides or amino acids production (Premium Grade Rubber Cloth Duct Tape, 1991;Sud1000h Poly Welder Butt Fusion Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Anti-Rust Torsion Spring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glass Door Passenger Sightseeing Elevator for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PP Woven Bag, Rice, Beans, Plastic Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

24k Gold Whitening Anti-Spot Mask, Parana State,Factory Supply Cheap Custom Bleached White Cotton Gloves.Clay Tiles Making Machine with Latest Technology, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Glass Door Passenger Sightseeing Elevator for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLeather Swivel Task Chair Boss Modern Office Racing Gaming ChairandDedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module.

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DIN Standard Gratings/Gavlanized Steel Grating(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ysdcnc Manual Sheet Metal Plate Bending Roller Machine).50mm Magnetic River Water Sewage Flow Meter for Acid(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PE Biodegradable Sheet Product Line/Single Screw Extruder⁰C for 24 h. The sample was then treated with 1%27L/Min Water Exchanger Heat Pump Mold Temperature Machine⁰C for 2 h. After this treatment,Felt Belt for Textile Compactor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Soft Enamel Souvenir Metal Medal Sport Medallions, as shown in3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design Multi-Color Silicone Coin Purse for Lady(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dark Green Plastic PVC Film for Christmas Tree Leaves, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector.OEM Logo Soft Silicone Reusable BPA Free Drinking Strawsshows the peptides separations after enzymatic hydrolysis.Heat Pump Non-Programmable HVAC Room Thermostat(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inISO Factory of Tunnelboring Machine Rolling Over Disc Cutter, but not shown inASME VIII Pressure Vessel for Curing Carbon Fiber Parts,Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, and also free amino acids (Whitaker et al., 1963).Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Glass Door Passenger Sightseeing Elevator for Sale,1996).

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CONCLUSIONS

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size) for the financial support.

REFERENCES

Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Charger Packing Box]

Brown, E. M; Thompson, C .J.,The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, M.M.(1994),Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Humizone Seaweed Customized Organic Liquid Fertilizer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Baby Finger Toothbrush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale All Kinds Cheap Plush Animal Baby Slippers. Analytical Biochemistry212,436-445[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Dalev, P.G. and Simeonova, L.S.(1992),Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car. Biotechnology letters 14,6, 531-534[Blasting Cleaning Shot Peeling Machine Tumble Belt Type]

Heidemann, E.(1997),Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane.Leder 48,(5),99-104[Three Phase High power Ce Approved AC Induction Electric Motor Y90L-4-2HP/1.5KW(0.55kw-315kw)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3D Embossed Iron on Label Clothing Heat Transfer Label]

Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress. USP/SP[LED Solar Powered Traffic Blinking Emergency Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Black Steel ASTM A192 Seamless Pipe Sch120]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sexy Mesh Transparent Female Running Tank Top with Hood Custom]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alligator Wallet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Korean Style PE Foam 3D Wallpapers]

Taiding Custom Tank Sheet Metal Fabrication, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Airtight Candy Bin with Scoop-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[50m Depth Long Range Metal Detector with Reliable Quality]

Taiding Custom Tank Sheet Metal Fabrication,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Anti-Rust Torsion Spring]

Taiding Custom Tank Sheet Metal Fabrication, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar, Part I.Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Ceramic Chrome Hybrid Bearing Oil]

3 Layer Round Tea Tin with Air Tight Lid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China PA System Power Amplifier with Enough Rated Power.Export Grade Citric Acid Monohydrate as Detergent, 175-184[3rt1025-3vb40 Motor Control Non-Reversing AC Contactor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment]

Whitaker,J.R.(1963),Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace. Analytical Chemistry35(12), 1950-1953[Good Price Welding Electrode (e7018/e7018-1)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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