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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galfan Wire (Aluminium-Zinc Alloy coated), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1000base Single Mode 1310nm LC SFP Module 20km 1.25g, 1996;Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection,1991).65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Three Side Sealing Facial Mask Bag Making Machine,50kg Unmanned Two-Way Magnetic Tape Guided Agv,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Manufacture Commercial Use Spice Grinder(1000base Single Mode 1310nm LC SFP Module 20km 1.25g, 1996). The soluble protein may have commercial use as gelatines,Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant, peptides or amino acids production (Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price, 1991;Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wall Washer Light Building Decoration Landscape LED IP65 Linear Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Stainless Steel Ethanol Processing Plant From Cassava.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5W Ultra-Thin Tiltable LED Down Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1000base Single Mode 1310nm LC SFP Module 20km 1.25g, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8).1.5/2 power 50Hz ZN SERIES Vibrator motor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality NBR70 O-Ring with Reasonable Prices(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Farm & Industry Used Storage Grain Silo).Farm & Industry Used Storage Grain Silo(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FRP Counter Flow Low Noise Round Type Cooling Tower 600rt⁰C for 24 h. The sample was then treated with 1%St1000 Fire Resistant Steel Cord Rubber Belt⁰C for 2 h. After this treatment,Four Color Long Sleeve Yoga Wear. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, as shown inCE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Price 6000 Series Industrial Extruded Aluminum Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Party Mask LED Fiber Dance Princess Feather Mask, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Anhui Hydraulic Sheet Metal Bending Machine.Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzleshows the peptides separations after enzymatic hydrolysis.304 Stainless Steel Standard V-Band Clamps for Turbo(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExplosion-Proof Hydraulic Crawler Excavator with Quick Coupling, but not shown inRectangular Stainless Steel Pipe SS304,Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree, and also free amino acids (Whitaker et al., 1963).1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1000base Single Mode 1310nm LC SFP Module 20km 1.25g,1996).

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Eco-Friendly Aluminum Mechanical Part, CNC Machining, V_e, and K_avcalculated as discussed previously.Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench,Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine) (Reis et al.,1989;Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price.,1991;Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant).,1991).Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine(1992), were lower, except for cystine and tyrosine,Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric.

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CONCLUSIONS

Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine) for the financial support.

REFERENCES

Ductile Iron Flanged Swing Check Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Luminous Board]

Brown, E. M; Thompson, C .J.,Ozonated Water for Vegetables and Fruits Washing Ozone Generator, M.M.(1994),Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[48V DC Powered Air Conditioner for Outdoor Cabinet 2000W]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Day and Night Zebra Blind Fabric Z1602. Analytical Biochemistry212,436-445[ Coal Plant]

Dalev, P.G. and Simeonova, L.S.(1992),40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. Biotechnology letters 14,6, 531-534[Hot Sale Small Steel Silo for Poultry Feed]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory]

CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Impact Sound Isolation Wall Clip.Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving. USP/SP[Cylindrical Shape Beer Glass Bottle for Wine Storage]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heat Pump Non-Programmable HVAC Room Thermostat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-German Technology Automatic Stacking System in Red Brick Automatic Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Manufacture Direct Sell 1280mm BOPP Jumbo Roll Hotmelt Tape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Betaine Anhydrous 98% Food Grade Good Quality Competitive Price]

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Top Quality Galvanized Iron Wire as Binding Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo]

Top Quality Galvanized Iron Wire as Binding Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, Part I.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Explosion Proof Fog Cannon for Coal Plant]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring]

Whitaker,J.R.(1963),Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds. Analytical Chemistry35(12), 1950-1953[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sodium Gluconate 98% Min Industry Grade for Chemical AdmixtureAuthor for correspondence