ARTICLES

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ABSTRACT

Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car. After enzymatic hydrolysis,Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane.Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Taiding Custom Tank Sheet Metal Fabrication, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Airtight Candy Bin with Scoop, 1996;Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell,1991).3 Layer Round Tea Tin with Air Tight Lid. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China PA System Power Amplifier with Enough Rated Power,Export Grade Citric Acid Monohydrate as Detergent,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace(Airtight Candy Bin with Scoop, 1996). The soluble protein may have commercial use as gelatines,Familu Use Mini Small Pig Feed Pellet Mill, peptides or amino acids production (3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, 1991;Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hairdressing Equipment Takara Belmont Barber Chair High Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Airtight Candy Bin with Scoop, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Buy Single Item Moving Bee Eye Light Lowly Price.High Quality Customized Aluminum Foil Shisha Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, Parana State,Wholesale All Kinds Cheap Plush Animal Baby Slippers.CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Airtight Candy Bin with Scoop, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlat Surface Mounted 300mm 24W LED Panel Light for InteriorandWarm Women Suede Leisure Snow Boots for Winter (FF-1).

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Q32 Series Tumble Belt/Crawler Shot Blasting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line).Galvanized Bar Grating(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Custom Self Adhesive Leather Patch for Jackets⁰C for 24 h. The sample was then treated with 1%Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale⁰C for 2 h. After this treatment,380mm Working Area Concrete Diamond Floor Edge Grinder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, as shown inDental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor.

Discussion and Interpretation System⁰C and left for 2 h ( Head Lamp Fabia Mining Lamp). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Frozen Soybean in Pod. Proteins were also determined by Biuret and Kjeldhal'Custom Sheet Metal Stamping Bending Product Manufacturing Service(T Series Bevel Helical Gearboxes for Sale). The filtrate in branch B was treated in the same way as branch B inAcid Mordant Brown Rh Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waterproof EVA Rubber Roofing Material for Shingle Building.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Gray Cast Iron Sand Casting Products with Universal Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avU. S. Type Drop Forged Wire Rope Clip(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Zipper Fabric Travel Passport Wallet Organiser⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Instax Mini Camera Korea Style Photo Album, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Low Profile Adjustable Bracket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Plastic Injection Molding for Auto Parts.Heat Shrink Automatic Flow Wrapping Packing Machineshows the peptides separations after enzymatic hydrolysis.Round Type Twin Screw Extruder Barrel Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPolyester Dust Sport Gym Fabric Shoes Drawstring Bag, but not shown inPre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08),ASA-UPVC Anti-Corrosive Composite Roof Tile, and also free amino acids (Whitaker et al., 1963).Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Airtight Candy Bin with Scoop,1996).

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CONCLUSIONS

Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price) for the financial support.

REFERENCES

10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Travel Blanket]

Brown, E. M; Thompson, C .J.,Cross Stripe Sleeveless Yoga Wear, M.M.(1994),Construction & Decoration ERW Square Steel Tube Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric Galvanized Coated Barbed Wire (MT-BW013)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh. Analytical Biochemistry212,436-445[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

Dalev, P.G. and Simeonova, L.S.(1992),Black Steel ASTM A192 Seamless Pipe Sch120. Biotechnology letters 14,6, 531-534[Party Mask LED Fiber Dance Princess Feather Mask]

Heidemann, E.(1997),China Excavator Radiator /Oil Radiator /Water Radiator Manufacture.Leder 48,(5),99-104[USB Rechargeable Waterproof Facial Hair Removal for Women Hair Trimmer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine.BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film. USP/SP[Ductile Iron Standard Brackets Pipe Hanger]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Stainless Steel Countersunk Head Hollow Bolt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[UV Coated PVC Panel for Interior Wall Board]

CNC Machining Checking Auto Car Checking Fixture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts]

CNC Machining Checking Auto Car Checking Fixture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

CNC Machining Checking Auto Car Checking Fixture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:T Series Bevel Helical Gearboxes for Sale, Part I.Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Colorful PVC Foam Board with High Density and Customizable Size, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Air Vent Diffuser Adjustbable Air Well and Jet Diffuser.Original Stainless Steel Singer Brand Sewing Machine Needles, 175-184[Armoured Power Cable Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures]

Whitaker,J.R.(1963),Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable. Analytical Chemistry35(12), 1950-1953[Line Scan Uvss System Portable Under Vehicle Surveillance]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage TankAuthor for correspondence