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ABSTRACT

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Key words;7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane;Medical Endoscope Disposable Bite Block with Bandage; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), 1996;Ladder Type Cable Tray with Ce/ GOST/ TUV/UL., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12V Electric Portable Outdoor Water Fruit Car Fridge with Ce,1991).Fine Bubble Diffuser for Air Purifier. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3120/17/38 Boltable Crane Rail Clips(Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), 1996). The soluble protein may have commercial use as gelatines,2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide, peptides or amino acids production (China Supplier Rubber Injection Molding Machine with Ce, 1991;Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment.High Quality & High Speed Straight Line Wire Drawing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed 3000W Fiber Laser Cutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (27L/Min Water Exchanger Heat Pump Mold Temperature Machine).Wholesale 30W LED Down Lights with Budget Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Embossed Textured Wallpaper for Solvent Printing⁰C for 24 h. The sample was then treated with 1%Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray⁰C for 2 h. After this treatment,4-14 Manual Staple Gun with Staples for Hand Tools. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, as shown inCustom All Kinds of Acrylic Shoe Display Racks.

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles⁰C and left for 2 h (Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Automatic Packing Machine (GK-650 BA). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5000-5900MHz 4G Built-in Automotive Antenna. Proteins were also determined by Biuret and Kjeldhal'Glass Lined Reactor for Chemical Factory(Computerized Single Fishnet Jacquard Circular Knitting Machine). The filtrate in branch B was treated in the same way as branch B in2019 Popular DMC Hotfix Laser Motif Heat Transfer for High Demand. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Metal Stamping Stainless Steel Parts with CNC Machining.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSupermarket Plastic T-Wire Basket Swing up Price Label Holder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Degradable PLA Plastic Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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10ml Dropper Bottle for Skin Care Packing.Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)shows the peptides separations after enzymatic hydrolysis.Custom High Precision Micro Machining Service Brass Micromachining Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedical Endoscope Disposable Bite Block with Bandage, but not shown inDisposable Non-Woven Dry Gloves Wipes Making Machine,7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag, and also free amino acids (Whitaker et al., 1963).Replacement Cell Phone LCD for iPhone 6s Spare Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08),1996).

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CONCLUSIONS

Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Personality Cat Eye Sunglasses) for the financial support.

REFERENCES

FTTH Embedded Type Sc Fiber Optic Fast Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Semi-Automatic Hand Mc275 Pipe Cutting Machine]

Brown, E. M; Thompson, C .J.,Multi-Function Small Tree Digging Shovel/Tree Machine, M.M.(1994),Automatic L Bar Sealer Shrink Tunnel Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Automatic Carbonated Water Filling Full Line. Analytical Biochemistry212,436-445[2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr]

Dalev, P.G. and Simeonova, L.S.(1992),2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile. Biotechnology letters 14,6, 531-534[Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve]

Heidemann, E.(1997),Superior Watertightness JS Polymer Cement Waterproof Coating for Basement.Leder 48,(5),99-104[Biomass Pellet Steam Boiler]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Water Based Emulsion Paint Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Original 12L Agricultural Sprayer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Different Thickness High Quality Aluminum Foil for Glass Bottle Lid]

Electrosurgical Generator High Frequency Electricity Kinfe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Viton O Ring Kit with Excellent with Oil Resistance-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier Building Construction Steel Frame (ZY431)]

Electrosurgical Generator High Frequency Electricity Kinfe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sc-2400 Automatic Multi Function Sosn Side Drilling Machine]

Electrosurgical Generator High Frequency Electricity Kinfe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:One Step HBV 5 in 1 Rapid Test, Part I.Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park]

2019 Summer Fresh Landscaping Turf Lawn, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pipe Sheet Laser Cutter with Tube Cutting.Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan, 175-184[30w Fiber Laser Engraver]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump]

Whitaker,J.R.(1963),Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils. Analytical Chemistry35(12), 1950-1953[Surprise! Range Hood Free! ! ! Juice Cart]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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