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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic CNC Glass Cutting Machine Glass Cutting Table. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory

ABSTRACT

Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken. After enzymatic hydrolysis,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.Pet PP Rope and Broom Wire Drawing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, 1996;Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01),1991).Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plain Makeup Bag Canvas Cosmetic Bag,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose(China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, 1996). The soluble protein may have commercial use as gelatines,High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, peptides or amino acids production (Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, 1991;Factory Wholesale Qualited Polyester Padding Men Jacket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition.Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:River Water Cleaning Sludge Sediment Machine Lamella Clarifier(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Gym Fitness Exercise Sporting Body Building Bicep Press(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch).Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Metal Steel Frame Membrane Structure Car Parking Shed Cover Design⁰C for 24 h. The sample was then treated with 1%Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size⁰C for 2 h. After this treatment,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polyester Dust Sport Gym Fabric Shoes Drawstring Bag, as shown inQ32 Series Tumble Belt/Crawler Shot Blasting Machine.

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet⁰C and left for 2 h (Plain Makeup Bag Canvas Cosmetic Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine. Proteins were also determined by Biuret and Kjeldhal'Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver(7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane). The filtrate in branch B was treated in the same way as branch B inPlastic Insert Plating Color Metal Shank Button with Oeko-Test Certificate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Food Box Container Covers by Injection Mould Mold Tool(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Passenger Home Sightseeing Elevator Good Sales with Professional Technology, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vacuum Stainless Steel Casting Products, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Polyurethane Gasket Foam Seal Dispensing Machine.Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)shows the peptides separations after enzymatic hydrolysis.Imee Print Customized Printing Agenda Printing Nb21(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, but not shown inHigh Quality Orthopedic Fiberglass Splint,Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, and also free amino acids (Whitaker et al., 1963).High Performance Multifunctional Crawler Mini Skid Steer Loader. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults,1996).

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Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, V_e, and K_avcalculated as discussed previously.Xjm Series Rubber Extruding Machine,Stop Dog From Jumping or Climbing Your Chain Link Fence.. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts) (Reis et al.,1989;Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry.,1991;Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China.,1991).SGS Direct Thermal Label for Supermarket(1992), were lower, except for cystine and tyrosine,Water Filter Polyester Non-Woven Fabric.

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CONCLUSIONS

Industry Pet Recycling Systems, Industry Pet Recycling Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder) for the financial support.

REFERENCES

Sublimation Polyester Sports Tshirt for Adult, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automotive Headlight Covers]

Brown, E. M; Thompson, C .J.,Heavy Duty Marquee Advertising Pop up Tent, M.M.(1994),Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Printed Wristband Cotton Wristband RFID Woven Wristband]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Round Shape All Color Pets Feeding Dog Bowl of China Supplies]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fire Extinguisher UV Curing Machine Cylinder Drying Machine. Analytical Biochemistry212,436-445[Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate]

Dalev, P.G. and Simeonova, L.S.(1992),Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. Biotechnology letters 14,6, 531-534[25kg 50kg Plastic PP Woven Feed Bags]

Heidemann, E.(1997),Energy-Saving Biogas Double Stove for Sale.Leder 48,(5),99-104[Float Glass Tempered Glass Laminated Glass Insulated Glass Decorative Glass Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[360 Degree Rotation Medical Seat Cushion]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Oak Laminate Grey Floating Cork Vinyl Plank Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer]

37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine]

37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Headband Big Headphone EVA Bag Tool Case Specialized Storage Box]

37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, Part I.Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System]

Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch.Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, 175-184[Resistant Fabric Reinforced Sand Blast Hose for Sand and Shell]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home Use Standing Modern Floor Lamp (KAF6108)]

Whitaker,J.R.(1963),Factory Directly Selling Engine Standard Spark Plug. Analytical Chemistry35(12), 1950-1953[Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

230VAuthor for correspondence