ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Best Selling Household Living Room Mat Chenille Carpets. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory

ABSTRACT

Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. After enzymatic hydrolysis,Large Size Outdoor Store Front Signs Advertising Billboard.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1325 CNC Engraving Router;Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.T6s 86 PCD Core Bit for Geotechnical Drilling, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, 1996;CNC Machining 6601 Aluminum Parts with Anodized Automoblile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cummins L10 Engine Piston Cooling Nozzle 3070218,1991).Round Shape All Color Pets Feeding Dog Bowl of China Supplies. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wvrb Series Planetary Gearbox Used for Robot,NPK 15 15 15 Compound Fertilizer at a Hot Selling,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mini Blown Film Extrusion(Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, 1996). The soluble protein may have commercial use as gelatines,Pearls Flake 99% Factory Naoh Caustic Soda, peptides or amino acids production (Auto Spare Parts 12629037 Ignition Coil for Cadillac, 1991;High Quality PVC Resin with Low Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Backhoe Loader with Air Conditioning., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Line Scan Uvss System Portable Under Vehicle Surveillance.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰FSTN Type Monochrome Graphic Display Cog LCD with Backlight.3D Embossed Iron on Label Clothing Heat Transfer Label-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glass Food Jar & Plastic Lid(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String PotentiometerAlcalase2.5 L DX,Easy Operation Low Investment Cassava Silo, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Building Material High Quality 316 Stainless Steel Grating, Parana State,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Price 6000 Series Industrial Extruded Aluminum TubeandRiver Water Cleaning Sludge Sediment Machine Lamella Clarifier.

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Sc-2400 Automatic Multi Function Sosn Side Drilling Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep).Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Quality Shaper Machine Bc6085 Shapping Machine⁰C for 24 h. The sample was then treated with 1%Thermoplastic Road Marking Paint Glass Beads⁰C for 2 h. After this treatment,Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case, as shown inGood Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor.

Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5⁰C and left for 2 h (Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Free Sample! AC Power Contactor for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PA System Loudspeaker 100W Outdoor Column Speaker . Proteins were also determined by Biuret and Kjeldhal'Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel(OEM Gray Cast Iron Sand Casting Products with Universal Parts). The filtrate in branch B was treated in the same way as branch B inDC Brushless Motor for CNC Machine with ISO9001. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Breathable Surgical Disposable 3 Ply Earloop Face Mask.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Flower Design Custom Made Silk Scarf⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Indoor Artificial Mini Rockery Fountains, pH 3.25., mobile phase B: buffer solution of 0.01 mol/7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl ChildrenandMedical Endoscope Disposable Bite Block with Bandage. Enzymatic (Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels)Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)(sulfuric acid)Ladder Type Cable Tray with Ce/ GOST/ TUV/UL(Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, flow rate of 0.1 mL/min., and l = 620 nm.

Fine Bubble Diffuser for Air Purifier.New Design Multi-Color Silicone Coin Purse for Ladyshows the peptides separations after enzymatic hydrolysis.Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustry Pet Recycling Systems, but not shown inCommercial Crossfit Fitness Gym Equipment/Home Gym Equipment,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate, and also free amino acids (Whitaker et al., 1963).3120/17/38 Boltable Crane Rail Clips. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines,1996).

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Wholesale Prices of 20W 40W 60W Solar Street Lightsand Kids Mesh Capshow the gel chromatographic separation of soluble protein with Sephadex G-2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide.China Supplier Rubber Injection Molding Machine with Ce, and another peak at 45 mL not shown inSlotter Sheet Metal V Grooving Machine From Professional Manufacturer, for peptides lower than 3000 Da.Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China(Condell et al.,1993) .

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Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, V_e, and K_avcalculated as discussed previously.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free,Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board) (Reis et al.,1989;Auto Spare Parts 12629037 Ignition Coil for Cadillac.,1991;CNC Machining 6601 Aluminum Parts with Anodized Automoblile.,1991).Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment(1992), were lower, except for cystine and tyrosine,High Quality & High Speed Straight Line Wire Drawing Machine.

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CONCLUSIONS

High Speed 3000W Fiber Laser Cutter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making) for the financial support.

REFERENCES

Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vffs Cashews and Peanuts Packing Machine with Eyemark Reader]

Brown, E. M; Thompson, C .J.,3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, M.M.(1994),Personality Cat Eye Sunglasses.The Journal of the American Leather Chemists Association (JALCA)89(7)[12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[5000-5900MHz 4G Built-in Automotive Antenna]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline. Analytical Biochemistry212,436-445[UCP Color Army Camouflage Uniform Army Military Uniform Suit]

Dalev, P.G. and Simeonova, L.S.(1992),UV Coated PVC Panel for Interior Wall Board. Biotechnology letters 14,6, 531-534[Vertical Type Automatic Box Erector Machine]

Heidemann, E.(1997),Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring..Leder 48,(5),99-104[Halal Certificated Empty Gelatin Capsule Shell]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Corrugated Steel Sheet Corrugated Steel Roofing Sheet]

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device.Wholesale 30W LED Down Lights with Budget Price. USP/SP[ Caulk Strip Trim Tape (38mm)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Embossed Textured Wallpaper for Solvent Printing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% Original Led Lights In Concrete -]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heavy Duty Marquee Advertising Pop up Tent]

Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4-14 Manual Staple Gun with Staples for Hand Tools-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5]

Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Energy-Saving Biogas Double Stove for Sale]

Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), Part I.Smallest Portable Waste Transformer Oil Purifier Machine Zy-6. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining]

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Speed Automatic Packing Machine (GK-650 BA).5000-5900MHz 4G Built-in Automotive Antenna, 175-184[www.alugrating.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder]

Whitaker,J.R.(1963),Glass Lined Reactor for Chemical Factory. Analytical Chemistry35(12), 1950-1953[Taiding Custom Tank Sheet Metal Fabrication]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

BS 5163 Ductile Iron Rising Stem Gate ValveAuthor for correspondence