ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump

ABSTRACT

10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. After enzymatic hydrolysis,300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction.BS 5163 Ductile Iron Rising Stem Gate Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Aluminum Sheet Press Brake;ID25mm Od86mm Standard Through Hole Slip Ring; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bio Media, 1996;Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Platform Trolley with 150kg Capacity, 4 Castor Wheel,1991).Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pre-Engineered Cage Broiler Poultry Farm Building(Bio Media, 1996). The soluble protein may have commercial use as gelatines,Alumina Ball with High Quality, peptides or amino acids production (PVC Textile Fabric for Outdoor Sports Waterproof Backpack, 1991;White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Powerful Anti-Stress, Strong Flexibility Spoke Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bio Media, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Modern American Style Beech Solid Wood Kitchen Cabinet.Hero Brand Polypropylene Bags Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machining Metal/Aluminum/Brass Auto Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.M4X10 Round Head Core Pulling Aluminum Nail⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Skytone 4 Channels 3500W Each DJ Power Amplifier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (316L Cold Rolled Stainless Steel Coil 316L Inox) for the financial support.

REFERENCES

Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[16oz Glass Beer Mug]

Brown, E. M; Thompson, C .J.,Cylindrical Shape Beer Glass Bottle for Wine Storage, M.M.(1994),Plastic Injection for OEM Product.The Journal of the American Leather Chemists Association (JALCA)89(7)[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Price Nasal Paste Packaging Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024. Analytical Biochemistry212,436-445[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event. Biotechnology letters 14,6, 531-534[Teerte Square Flat Head Self Tapping Screw]

Heidemann, E.(1997),Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen.Leder 48,(5),99-104[PVC Blank RFID MIFARE Classic 1K Contactless NFC Business Cards]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Activated Carbon Strong Effect Peeling off Face Mask Mud.Type Price Prefabricated Roof Tile in Sudan. USP/SP[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[13.56 MHz Fudan FM11RF08 Chip Card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chicken Battery Cage Chicken Layer Cage for Sale in Thailand]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

Factory Supply 100% Natural Epimedium Extract for Male Health, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Colombia Outdoor P5 Pantallas LED Display]

Factory Supply 100% Natural Epimedium Extract for Male Health,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Package Aluminum Foil Tray Making Machine]

Factory Supply 100% Natural Epimedium Extract for Male Health, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, Part I.Top10 1080P H. 264 P2p CCTV WiFi IP Camera. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5W Ultra-Thin Tiltable LED Down Light]

Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturer Custom Precision Aluminum Die Casting LED Light Housing.Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate, 175-184[Korea Steel Grating]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electrophoresis Assembly Infilltration Tank for Effluent Treatment]

Whitaker,J.R.(1963),1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S). Analytical Chemistry35(12), 1950-1953[PA System Loudspeaker 100W Outdoor Column Speaker ]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bsp Standard Malleable Iron Pipe Fitting Hexagon NippleAuthor for correspondence