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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-1325 CNC Engraving Router. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel

ABSTRACT

T6s 86 PCD Core Bit for Geotechnical Drilling. After enzymatic hydrolysis,Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.CNC Machining 6601 Aluminum Parts with Anodized Automoblile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wvrb Series Planetary Gearbox Used for Robot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (NPK 15 15 15 Compound Fertilizer at a Hot Selling, 1996;Mini Blown Film Extrusion., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pearls Flake 99% Factory Naoh Caustic Soda,1991).Auto Spare Parts 12629037 Ignition Coil for Cadillac. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality PVC Resin with Low Price,Small Backhoe Loader with Air Conditioning,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Line Scan Uvss System Portable Under Vehicle Surveillance(NPK 15 15 15 Compound Fertilizer at a Hot Selling, 1996). The soluble protein may have commercial use as gelatines,Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, peptides or amino acids production (FSTN Type Monochrome Graphic Display Cog LCD with Backlight, 1991;3D Embossed Iron on Label Clothing Heat Transfer Label, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glass Food Jar & Plastic Lid., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Easy Operation Low Investment Cassava Silo. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (NPK 15 15 15 Compound Fertilizer at a Hot Selling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Building Material High Quality 316 Stainless Steel Grating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo).I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5⁰C for 24 h. The sample was then treated with 1%Free Sample! AC Power Contactor for Sale⁰C for 2 h. After this treatment,PA System Loudspeaker 100W Outdoor Column Speaker . The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Breathable Surgical Disposable 3 Ply Earloop Face Mask, as shown inExplosion Proof Fog Cannon for Coal Plant.

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9⁰C and left for 2 h (Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. Proteins were also determined by Biuret and Kjeldhal'Flower Design Custom Made Silk Scarf(Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt). The filtrate in branch B was treated in the same way as branch B inProducts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Design Indoor Artificial Mini Rockery Fountains.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedical Endoscope Disposable Bite Block with Bandage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, pH 3.25., mobile phase B: buffer solution of 0.01 mol/12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Supplier Rubber Injection Molding Machine with Ce.Alumina Ball with High Qualityshows the peptides separations after enzymatic hydrolysis.Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Automotive Water Pump Seal, but not shown inMulticore Underground Aluminum Conductor Steel Tape Armored Power Cable,Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, and also free amino acids (Whitaker et al., 1963).High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (NPK 15 15 15 Compound Fertilizer at a Hot Selling,1996).

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330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm FencingandEco-Friendly Recycling PS Foam Container Production Lineshow the gel chromatographic separation of soluble protein with Sephadex G-Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, and another peak at 45 mL not shown inMedical Packing Bags, for peptides lower than 3000 Da.High Quality & High Speed Straight Line Wire Drawing Machine(Condell et al.,1993) .

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CONCLUSIONS

UV Coated PVC Panel for Interior Wall Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair) for the financial support.

REFERENCES

Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring., A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aging Food Grade Hydrolyzed Fish Collagen Supplier & Factory]

Brown, E. M; Thompson, C .J.,Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, M.M.(1994),Wholesale 30W LED Down Lights with Budget Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Customized Aluminum Foil Shisha Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Embossed Textured Wallpaper for Solvent Printing. Analytical Biochemistry212,436-445[High Quality Mini LED Flood Light SMD Flood Light 50W]

Dalev, P.G. and Simeonova, L.S.(1992),Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray. Biotechnology letters 14,6, 531-534[Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Anti-High Pressure Insulated Pliers with PVC Dipped Handles]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pre-Engineered Cage Broiler Poultry Farm Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric Motor Materials]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides]

High Speed Automatic Packing Machine (GK-650 BA), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5000-5900MHz 4G Built-in Automotive Antenna-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Amazon Hot Selling Kids Playing Pop up Goal]

High Speed Automatic Packing Machine (GK-650 BA),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla]

High Speed Automatic Packing Machine (GK-650 BA), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Glass Lined Reactor for Chemical Factory, Part I.OEM Metal Stamping Stainless Steel Parts with CNC Machining. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65]

Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Supermarket Plastic T-Wire Basket Swing up Price Label Holder.1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, 175-184[Carving Imitate Corbel High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper]

Whitaker,J.R.(1963),Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7. Analytical Chemistry35(12), 1950-1953[Steel Bar Peeling Lathe Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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