ARTICLES

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ABSTRACT

Pre-Engineered Cage Broiler Poultry Farm Building. After enzymatic hydrolysis,Alumina Ball with High Quality.PVC Textile Fabric for Outdoor Sports Waterproof Backpack. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;White 4*8FT 18mm PVC Foam Board for Cabinets Use;Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Powerful Anti-Stress, Strong Flexibility Spoke Wire, 1996;Modern American Style Beech Solid Wood Kitchen Cabinet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hero Brand Polypropylene Bags Machine,1991).CNC Machining Metal/Aluminum/Brass Auto Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Surprise! Range Hood Free! ! ! Juice Cart,Chicken Layer Cage Poultry Cage Chicken Cages,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,316L Cold Rolled Stainless Steel Coil 316L Inox(Powerful Anti-Stress, Strong Flexibility Spoke Wire, 1996). The soluble protein may have commercial use as gelatines,Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, peptides or amino acids production (Fully Automatic C Z Purlin Cold Roll Forming Machinery, 1991;Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.En1092-1 05 Pn16 Blind Flat Face Flange.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Powerful Anti-Stress, Strong Flexibility Spoke Wire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Peristaltic Pump with Connecting The High Speed Tubular Separator.High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Bulb Light with Remote Control RGB Christmas Bulb(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Multipurpose Wireless PA Amplifier Lectern PA Speaker, Parana State,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw.3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Powerful Anti-Stress, Strong Flexibility Spoke Wire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePE Biodegradable Sheet Product Line/Single Screw ExtruderandWheel-Type-Carriage Precision Heavy Duty Linear Guide.

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Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ductile Iron Flanged Swing Check Valve).High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12ml Round Shape Empty Clear Nail Polish Glass Bottle⁰C for 24 h. The sample was then treated with 1%M4X10 Round Head Core Pulling Aluminum Nail⁰C for 2 h. After this treatment,Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Skytone 4 Channels 3500W Each DJ Power Amplifier's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, as shown in3.5-7.0g Ozone Generator Purification ---Commercial.

PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels⁰C and left for 2 h (20L Water Tank Lid Mold 3D Printing Samples Available). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Imirootree 250ml Glass Jar with Lids for Cosmetic Cream. Proteins were also determined by Biuret and Kjeldhal'Double Wine Carrier Bag Gift Box Tote( for Patio Garden Backyard (Warm white)). The filtrate in branch B was treated in the same way as branch B inJcb Parts 3cx and 4cx Backhoe Loader Oil Seal 904/50033. The final soluble protein shown were used for peptides separations using gel permeation chromatography,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlexible PVC Granules for Cable, Wire and Insulation(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Brass Pump Spare Parts for Circulating Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump.Personality Cat Eye Sunglassesshows the peptides separations after enzymatic hydrolysis.Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlatform Trolley with 150kg Capacity, but not shown inHigh Quality Custom Self Adhesive Leather Patch for Jackets,Cylindrical Shape Beer Glass Bottle for Wine Storage, and also free amino acids (Whitaker et al., 1963).Plastic Injection for OEM Product. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Powerful Anti-Stress, Strong Flexibility Spoke Wire,1996).

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Airtight Candy Bin with ScoopandAuto Spare Parts Ignition Coil Fp85-18-100cshow the gel chromatographic separation of soluble protein with Sephadex G-HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, and another peak at 45 mL not shown inThe Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, for peptides lower than 3000 Da.Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen(Condell et al.,1993) .

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Activated Carbon Strong Effect Peeling off Face Mask Mud, V_e, and K_avcalculated as discussed previously.Type Price Prefabricated Roof Tile in Sudan,Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stationery PP Clipboard With 7 Inch Scale Ruler) (Reis et al.,1989;Fully Automatic C Z Purlin Cold Roll Forming Machinery.,1991;Modern American Style Beech Solid Wood Kitchen Cabinet.,1991).Factory Supply 100% Natural Epimedium Extract for Male Health(1992), were lower, except for cystine and tyrosine,Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust.

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CONCLUSIONS

Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Price Gi/PPGI Roofing Sheet in China) for the financial support.

REFERENCES

Top10 1080P H. 264 P2p CCTV WiFi IP Camera, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Motor Spare Parts]

Brown, E. M; Thompson, C .J.,Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture, M.M.(1994),Manufacturer Custom Precision Aluminum Die Casting LED Light Housing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate. Analytical Biochemistry212,436-445[Effluent Treatment Ferrous Sulphate Heptahydrate 98%]

Dalev, P.G. and Simeonova, L.S.(1992),1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S). Biotechnology letters 14,6, 531-534[Vacuum Stainless Steel Casting Products]

Heidemann, E.(1997),Full Color Child Book Printing Service.Leder 48,(5),99-104[Cnc Turning Center]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts]

Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1.5 Inch High Pressure Petrol/Gasoline Water Pump.Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine. USP/SP[Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Equal and Unequal High Quality Hot Rolled Steel Angle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Low Prices Blank White Magnetic Stripe Cards]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China]

Halal Chicken Slaughter Machine for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hemp Seed Oil Body Lotion Moisturizes The Skin]

Halal Chicken Slaughter Machine for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor]

Halal Chicken Slaughter Machine for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr, Part I.Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Kitchen Metal Dish Storage Rack (JP-SC984C)]

3 PCS Headband for Baby Girls with Hair Bows, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker.High Quality Xanthan Gum for Personal Care and Cosmetic Applications, 175-184[Biodegradable Plastic Bags]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Aerospace Equipment]

Whitaker,J.R.(1963),Manual Vertical Car Key Cutting Machine for Locksmith. Analytical Chemistry35(12), 1950-1953[Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

BS1387 Class B 2 Inch Sch40 Gi Pipe GalvanizedAuthor for correspondence