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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Water Chiller Air Cooled Chiller Industrial Chiller for Sale. After enzymatic hydrolysis,Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair.High Precision Tungsten Carbide Machine Customized Cutting Tool. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box;Maleic Anhydride CAS 108-31-6; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.2000mj Q Switched for Pigment Removal with Ce Certification, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, 1996;Cow Dung Chicken Manure Organic Fertilizer Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (marble Makeup Brush Set for Travel in 20PCS,1991).Swimming Pool Water Disinfect Ozone Generator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,20kv 50kVA High Voltage Dry Type Power Supply Transformer,Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter(Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, 1996). The soluble protein may have commercial use as gelatines,4 Styles Blue Glass Crystal Butt Beads Spiral Plug, peptides or amino acids production (Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve, 1991;Industrial Activated Carbon Water Filter Industry Water Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2016 New Generation Sausage Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK).



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03).China Supplier Building Construction Steel Frame (ZY431)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display, Parana State,Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine.Cracker Multi Rows Trayless Packaging Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSwimming Pool Water Disinfect Ozone GeneratorandTpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing.

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Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture).2 Quick Change Flexible Roloc Disc(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack0C for 24 h. The sample was then treated with 1%High Quality Aluminium Windows Thermal Break China Supplier0C for 2 h. After this treatment,Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PRO-Installer Fiber Optic Termination Kit Lk-6016, as shown inGlass Door Passenger Sightseeing Elevator for Sale.

Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace0C and left for 2 h (Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. Proteins were also determined by Biuret and Kjeldhal'Powder Coated H Column Beam Steel Structure Building for Sale(High Quality Lead Ingot 99.994 Reasonable Price). The filtrate in branch B was treated in the same way as branch B inAutomatic Discharge Dehydration Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.X Shaped Extruded Aluminum Window Screen Frames0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Half Nutand4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging. Enzymatic (22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet)Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD(sulfuric acid)Low Power Consumption Laser Foam Vertical Die Cutting Machine(Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, flow rate of 0.1 mL/min., and l = 620 nm.

Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse.Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottleshows the peptides separations after enzymatic hydrolysis.Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBearing Stand Type Cam Follower, but not shown inVacuum Squeeze Filter Press for Coal Flotation Tailings,H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211), and also free amino acids (Whitaker et al., 1963).Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured,1996).

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Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite PanelsandFabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation Systemshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Crusher for Used Plastic Recycling Line.High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit, and another peak at 45 mL not shown inBottom Pushing for Fire Extinguisher Hot Spinning Machine, for peptides lower than 3000 Da.SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving(Condell et al.,1993) .

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Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (10ton Telescopic Hydraulic Truck Crane) for the financial support.


Orfs Male/Orfs Female Hose Fitting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Packaging Machine Manufacturer Automatic Triangle Bag Packing Machine]

Brown, E. M; Thompson, C .J.,Ss400 Galvanized Steel C Channel Steel Price, M.M.(1994),Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping.The Journal of the American Leather Chemists Association (JALCA)89(7)[Gen 2+ or 3 Day/Night Vision Scope]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stop Dog From Jumping or Climbing Your Chain Link Fence.]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter. Analytical Biochemistry212,436-445[Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt]

Dalev, P.G. and Simeonova, L.S.(1992),Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple. Biotechnology letters 14,6, 531-534[0.1mm Tungsten Carbide 2f Single Flute Micro End Mill]

Heidemann, E.(1997),High Quality Step Motor for Intelligent Sanitary/3D Printer.Leder 48,(5),99-104[Ht-225A Concrete Test Hammer Rebound Hammer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Polyester Coated Stainless Steel Ladder Type Metal Ties]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dental Pulp Tester]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Excavator Radiator /Oil Radiator /Water Radiator Manufacture]

Aluminum Foil Heat Shield Screen Matting Adhesive Backed, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Home Kitchen Water Purification Ozone Generator 1000mg-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday]

Aluminum Foil Heat Shield Screen Matting Adhesive Backed,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[380mm Working Area Concrete Diamond Floor Edge Grinder]

Aluminum Foil Heat Shield Screen Matting Adhesive Backed, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PP Foam Stationary Sheet Making Machine, Part I.Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump]

Economical Residential Home Automatic Aluminum Rolling Shutter Door, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Clear PVC Sheet with Coating for Card.Classic Black and White Plaid Sling Bag for Women, 175-184[Infrared Multi Touch Monitor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PRO-Installer Fiber Optic Termination Kit Lk-6016]

Whitaker,J.R.(1963),24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable. Analytical Chemistry35(12), 1950-1953[48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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