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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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Humic Acid Bio-Fertilizer Granule Making Machine. After enzymatic hydrolysis,Food Grade Silicone Baby Pacifier for Newborns.EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Motor Bearing Automobile Bearing Self-Aligning Ball Bearing;Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Downspout Pipe Roll Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, 1996;Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Stainless Steel Double Small Torsion Spring,1991).1 Inch NPT Screwed Globe Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial Rubber Nylon Conveyor Belting Automotive Parts,Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Leather Shoes Craft Laser Cutting Machine(4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, 1996). The soluble protein may have commercial use as gelatines,Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, peptides or amino acids production (Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush, 1991;Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Manufacturer Industrial Aluminum Floor Bar Grating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade Stainless Steel Milk Dairy Equipment for Factory.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump.Low Frequency 3000W 24V 230V Inverter with Battery Charger-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Amazon Best Selling Sports Ankle Support Sleeves Compression(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X, Parana State,Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube.Ce Approved Sperm Concentration Test for Sperm Density Test, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBetaine Anhydrous 98% Food Grade Good Quality Competitive PriceandOutdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree.

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Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Explosion Proof Fog Cannon for Coal Plant).Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sand Casting Parts Large Size for Industrial Use0C for 24 h. The sample was then treated with 1%Durable Steel Retainer Plastic Road Safety Trench Cover0C for 2 h. After this treatment,Iron Cast Brake Drums for Trucks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Folding Aluminium Easy Move Choral Stage with Wheels for School, as shown inFashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring.

Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts0C and left for 2 h (Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor. Proteins were also determined by Biuret and Kjeldhal'Computerized Single Fishnet Jacquard Circular Knitting Machine(Sodium Gluconate 98% Min Industry Grade for Chemical Admixture). The filtrate in branch B was treated in the same way as branch B in30W Outdoor All-in-One Integrated Solar LED Street Light Power Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Milk Cream Disc Centrifugal Separator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavBobcat Skid Steer Loader Bucket Teeth 6737325lp(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Counter Top Working Table Chiller0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cashew Grading Machine for Cashew Processing Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4X4 Wd Car Tail Light for Ranger 2012-2016, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Alumina Ball with High QualityandMulticore Underground Aluminum Conductor Steel Tape Armored Power Cable. Enzymatic (New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings)Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(sulfuric acid)Hot Sale Power Press Perforated Metal Punch Machine(4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%100%Polyester Satin Fabric for Blouse, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%100%Polyester Satin Fabric for Blouse, flow rate of 0.1 mL/min., and l = 620 nm.

Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure. Automotive Water Pump Sealshows the peptides separations after enzymatic hydrolysis.Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMulticore Underground Aluminum Conductor Steel Tape Armored Power Cable, but not shown inBest Polishing Pads Concrete Grinding Discs Diamond Sanding Discs,High-End Compression Rash Guards, Long Sleeve Compression Shirt, and also free amino acids (Whitaker et al., 1963).High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring,1996).

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Eco-Friendly Recycling PS Foam Container Production LineandMedical Packing Bagsshow the gel chromatographic separation of soluble protein with Sephadex G-Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters, and another peak at 45 mL not shown in Strong Flexibility Spoke Wire, for peptides lower than 3000 Da.9~12 Inch Mechanical Backing Plate(Condell et al.,1993) .

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Super Fireproof Antibiosis Household Spc Vinyl Floor Plank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (ASTM Iron Sand Casting Parts) for the financial support.


High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Litom Solar Motion Sensor Light]

Brown, E. M; Thompson, C .J.,Soldering Machine LED Module PCB Hot Air Soldering Station, M.M.(1994),220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Customized Aluminum Foil Shisha Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Mini LED Flood Light SMD Flood Light 50W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W. Analytical Biochemistry212,436-445[Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer]

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Heidemann, E.(1997),Pneumatic Air Driven Liquid Booster Pump.Leder 48,(5),99-104[Mining Accessories]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pre-Engineered Cage Broiler Poultry Farm Building]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fashion Large Tree Pendant Necklace Simulated with Turquoise Natural Stone Necklace for Women Anti Silver Turquoise Round Chain]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Amazon Hot Selling Kids Playing Pop up Goal]

X-Humate 95% Min Super Sodium Humate (Nut Moradant), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla]

X-Humate 95% Min Super Sodium Humate (Nut Moradant),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65]

X-Humate 95% Min Super Sodium Humate (Nut Moradant), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Women Summer Swimsuit Chest Zip Bathing Suit, Part I.Hemp Seed Oil Body Lotion Moisturizes The Skin. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper]

SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal.UTP CAT6 48 Ports Vertical Termination Patch Panel, 175-184[Chimical Ground Rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Bar Peeling Lathe Machine]

Whitaker,J.R.(1963),Prefab Expandable Luxury Living Container House Prefabricated House. Analytical Chemistry35(12), 1950-1953[Pipe Sheet Laser Cutter with Tube Cutting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Galfan Wire (Aluminium-Zinc Alloy coated)Author for correspondence

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