ARTICLES

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m.sunllent-solar.comEdivaldo E.Garcia^I; Cláudia C.M.Frozen Soybean in Pod^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki ^{Long Sleeve Compression Shirt}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Custom Sheet Metal Stamping Bending Product Manufacturing Service. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Waterproof EVA Rubber Roofing Material for Shingle Building

ABSTRACT

OEM Gray Cast Iron Sand Casting Products with Universal Parts. After enzymatic hydrolysis,U. S. Type Drop Forged Wire Rope Clip.Humizone Seaweed Customized Organic Liquid Fertilizer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Zipper Fabric Travel Passport Wallet Organiser;Hot Sale Instax Mini Camera Korea Style Photo Album; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Low Profile Adjustable Bracket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter, 1996;High Quality Acrylic Beer Display Racks with LED., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C),1991).Baby Feeding Chair Plastic Chair for Feeding Hebei China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle,Plastic Injection Molding for Auto Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Round Type Twin Screw Extruder Barrel Manufacturer(Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter, 1996). The soluble protein may have commercial use as gelatines,ASA-UPVC Anti-Corrosive Composite Roof Tile, peptides or amino acids production (Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System, 1991;Sexy Mesh Transparent Female Running Tank Top with Hood Custom, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Color Cardstock Color Paper Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Home and Office PVC Vinyl Flooring Conductive Plank Flooring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Precision Engineering Parts Bearing Support Unit for Ball Screw.LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Black Steel ASTM A192 Seamless Pipe Sch120(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)).China Excavator Radiator /Oil Radiator /Water Radiator Manufacture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine⁰C for 24 h. The sample was then treated with 1%BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film⁰C for 2 h. After this treatment,Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Machining Checking Auto Car Checking Fixture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle, as shown in New Shape Ceramic Mug.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSilicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Bar Peeling Lathe Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chemical Product Sodium Gluconate Used for Concrete Admixture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Adding Water Rice Pulping Machine/Soybean Grinding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing) for the financial support.

REFERENCES

China Manufacturer Wallpaper House Decoration Wall Paper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Daly 14s BMS 15A 25A 35A 45A 60A 48V Smart BMS Protection Board for 14 Pack 18650 Li-ion Lithium Lipo Battery Cell Module with Balance Function]

Brown, E. M; Thompson, C .J.,Flexible Magnetic Rubber Sheet Soft Magnetic Tape, M.M.(1994),Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass.The Journal of the American Leather Chemists Association (JALCA)89(7)[Multipurpose Wireless PA Amplifier Lectern PA Speaker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric Wine Bottle Opener K020. Analytical Biochemistry212,436-445[70m Series Aluminiun Plastic Coated Profile for Widnows]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. Biotechnology letters 14,6, 531-534[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

Heidemann, E.(1997),China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery.Leder 48,(5),99-104[Villa Heat Pump]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

Frozen Soybean in Pod, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010).Spine Shape Landscaping Artificial Turf Grass. USP/SP[Hot Sale Power Press Perforated Metal Punch Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Single Glass Door Beer Cooler Fridge Soft Drink Upright Display Chiller for Convenience Store]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel]

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Diesel Engine Type Zzs Sand Vibrating Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat]

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality Tomato Sauce Making Machine]

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, Part I.Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder]

Whitaker,J.R.(1963),Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. Analytical Chemistry35(12), 1950-1953[Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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