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ABSTRACT

Stainless Steel Ozone Disinfection Machine Ozone Generator. After enzymatic hydrolysis,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube.PVC Profile with Net Making Machine Used for Window Sealing Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, 1996;Diameter 2.68m Paragraphed Autoclave for AAC Bricks., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety,1991).Travelling Weekender Mens Canvas Duffle Gym Sports Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box,China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Selling Household Living Room Mat Chenille Carpets(Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, 1996). The soluble protein may have commercial use as gelatines,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, peptides or amino acids production (Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, 1991;Large Size Outdoor Store Front Signs Advertising Billboard, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1325 CNC Engraving Router.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰T6s 86 PCD Core Bit for Geotechnical Drilling.Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machining 6601 Aluminum Parts with Anodized Automoblile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mini Blown Film Extrusion, Parana State,Square Plastic PP Geocomposite Drainage Blind Ditch/Drain.Pearls Flake 99% Factory Naoh Caustic Soda, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMetal Trapezoidal Roof Sheet Roll Forming Machine for Roof PanelandDouble Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat.

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Auto Spare Parts 12629037 Ignition Coil for Cadillac(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine).High Quality PVC Resin with Low Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small Backhoe Loader with Air Conditioning⁰C for 24 h. The sample was then treated with 1%Line Scan Uvss System Portable Under Vehicle Surveillance⁰C for 2 h. After this treatment,Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FSTN Type Monochrome Graphic Display Cog LCD with Backlight's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Embossed Iron on Label Clothing Heat Transfer Label, as shown in2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder.

Glass Food Jar & Plastic Lid⁰C and left for 2 h (Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Easy Operation Low Investment Cassava Silo. Proteins were also determined by Biuret and Kjeldhal'CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making(Hot Sale Thermostat Electric Heating Element for Solar Water Heaters). The filtrate in branch B was treated in the same way as branch B inCast Steel Gate Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Building Material High Quality 316 Stainless Steel Grating. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDental Laboratory Technician Workstation Dental Technician Bench Technician Work Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quality Shaper Machine Bc6085 Shapping Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Thermoplastic Road Marking Paint Glass Beads, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manufacturer Custom Precision Aluminum Die Casting LED Light HousingandAutomatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. Enzymatic (Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts)I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case(sulfuric acid)Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5(Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Free Sample! AC Power Contactor for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Free Sample! AC Power Contactor for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

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New Design Indoor Artificial Mini Rockery Fountains, V_e, and K_avcalculated as discussed previously.7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane,Medical Endoscope Disposable Bite Block with Bandage. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle) (Reis et al.,1989;Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment.,1991;Diameter 2.68m Paragraphed Autoclave for AAC Bricks.,1991).Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels(1992), were lower, except for cystine and tyrosine,Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08).

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CONCLUSIONS

Ladder Type Cable Tray with Ce/ GOST/ TUV/UL. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wvrb Series Planetary Gearbox Used for Robot) for the financial support.

REFERENCES

12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[sincere-paper]

Brown, E. M; Thompson, C .J.,Fine Bubble Diffuser for Air Purifier, M.M.(1994),Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. Analytical Biochemistry212,436-445[Hot Sale Ultra Slim Elevator Digital Signage]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Fiber Laser Cutting Machine for Sheet and Tube]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Kwikstage Scaffolding System-Diagonal Brace]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chenille Microfiber Mop Head]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bathroom Mirror Cabinet With Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water]

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High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Common Rail Injector Tester]

High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, Part I.High Quality & High Speed Straight Line Wire Drawing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Peristaltic Pump with Connecting The High Speed Tubular Separator]

Whitaker,J.R.(1963),Personality Cat Eye Sunglasses. Analytical Chemistry35(12), 1950-1953[3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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