ARTICLES

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ABSTRACT

Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. After enzymatic hydrolysis,Linen Quiltcover Set with Quilting.2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace;Teerte Square Flat Head Self Tapping Screw; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bto 22 Electric Galvanized Razor Concertina Wire for Military, 1996;Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine,1991).Hot Selling Military Large Event Marquee Tent Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High-Quality Pre-Seasoned Cast Iron Wok,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine(Bto 22 Electric Galvanized Razor Concertina Wire for Military, 1996). The soluble protein may have commercial use as gelatines,Marine High Visibility Solas Reflective Tape, peptides or amino acids production (24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, 1991;Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Quality Waterproof IP66 Aluminum Die Cast Junction Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Magic Tape Hook and Loop Fasteners.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bto 22 Electric Galvanized Razor Concertina Wire for Military, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Starch Doy Bag Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, Parana State,Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44).Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bto 22 Electric Galvanized Razor Concertina Wire for Military, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePassport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525andDo Not Enter Tape Warning Tape Caution Tape Danger Tape.

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Factory Supply Cheap Custom Bleached White Cotton Gloves(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine).2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%304 &314 Stainless Steel Seamless Bending Tube⁰C for 24 h. The sample was then treated with 1%Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark⁰C for 2 h. After this treatment,Custom Grey Full-Zip Cotton Mens Hoodies. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)7inch Industrial Tablet Computer Naked Resistance Touch Screen, as shown in2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr.

Glass Door Passenger Sightseeing Elevator for Sale⁰C and left for 2 h (Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor. Proteins were also determined by Biuret and Kjeldhal'Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple(ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe). The filtrate in branch B was treated in the same way as branch B inAutomatic Foam Cut. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fcy30 Transport Mini Tipping Dumper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPtj601 Model 4 Post Storage Cars Parking Elevator for Auto Park(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Premium Grade Rubber Cloth Duct Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sud1000h Poly Welder Butt Fusion Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Anti-Rust Torsion Spring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Powder Coated H Column Beam Steel Structure Building for SaleandAluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. Enzymatic (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt)Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60(sulfuric acid)OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic(Bto 22 Electric Galvanized Razor Concertina Wire for Military, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PP Woven Bag, Rice, Beans, Plastic Bag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PP Woven Bag, Rice, Beans, Plastic Bag, flow rate of 0.1 mL/min., and l = 620 nm.

China Professional Mold Factory Plastic Injection Mould Maker.Water Based Emulsion Paint Production Lineshows the peptides separations after enzymatic hydrolysis.Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8, but not shown inDifferent Thickness High Quality Aluminum Foil for Glass Bottle Lid,Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, and also free amino acids (Whitaker et al., 1963).Industrial Carbon Steel Wire Hose Hydraulic Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bto 22 Electric Galvanized Razor Concertina Wire for Military,1996).

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50mm Magnetic River Water Sewage Flow Meter for Acid, V_e, and K_avcalculated as discussed previously.PE Biodegradable Sheet Product Line/Single Screw Extruder,27L/Min Water Exchanger Heat Pump Mold Temperature Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light) (Reis et al.,1989;24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.,1991;Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.,1991).Felt Belt for Textile Compactor(1992), were lower, except for cystine and tyrosine,Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt.

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CONCLUSIONS

Metal Soft Enamel Souvenir Metal Medal Sport Medallions. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper) for the financial support.

REFERENCES

20L Water Tank Lid Mold 3D Printing Samples Available, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pn 1.0mpa Flange]

Brown, E. M; Thompson, C .J.,Wholesale High Quality Metal Car Mount Holder Phone Holder, M.M.(1994),Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hexagonal Netting/ Gabion Box/ Gabion Basket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sand Casting Parts Large Size for Industrial Use]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. Analytical Biochemistry212,436-445[Auto Two Piece Folder Gluer for Corrugated Box]

Dalev, P.G. and Simeonova, L.S.(1992),Pet Laminate Aluminium Foil/Pet+Alu+PE. Biotechnology letters 14,6, 531-534[Factory Directly Selling Engine Standard Spark Plug]

Heidemann, E.(1997),6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.Leder 48,(5),99-104[220v 3014 Flexible Led Strip Lights]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box]

Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Design Multi-Color Silicone Coin Purse for Lady.Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. USP/SP[99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Women Summer Swimsuit Chest Zip Bathing Suit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flr2X-B XLPE Insulation 1.5mm2 Vehicle Automotive Cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065]

Dark Green Plastic PVC Film for Christmas Tree Leaves, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cnm Single Strand Anchor Coupler]

Dark Green Plastic PVC Film for Christmas Tree Leaves,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Design Underwear for Men Cotton Mens Boxer Briefs]

Dark Green Plastic PVC Film for Christmas Tree Leaves, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), Part I.1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel]

Water Treatment Anthracite, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heat Treated Welding Protection Glassfiber Cloth Ht800.Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, 175-184[Wholesale Polyester DIY Felt Christmas Decoration]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IEC 62196-2 Type 2 EV Charging 32A Male Plug]

Whitaker,J.R.(1963),Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. Analytical Chemistry35(12), 1950-1953[10mm Red Green Yellow Warning China Supplier Signal Lamp]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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