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ABSTRACT

1325 CNC Engraving Router. After enzymatic hydrolysis,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel.T6s 86 PCD Core Bit for Geotechnical Drilling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cummins L10 Engine Piston Cooling Nozzle 3070218, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Round Shape All Color Pets Feeding Dog Bowl of China Supplies, 1996;Wvrb Series Planetary Gearbox Used for Robot., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (NPK 15 15 15 Compound Fertilizer at a Hot Selling,1991).Mini Blown Film Extrusion. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pearls Flake 99% Factory Naoh Caustic Soda,Auto Spare Parts 12629037 Ignition Coil for Cadillac,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality PVC Resin with Low Price(Round Shape All Color Pets Feeding Dog Bowl of China Supplies, 1996). The soluble protein may have commercial use as gelatines,Small Backhoe Loader with Air Conditioning, peptides or amino acids production (Line Scan Uvss System Portable Under Vehicle Surveillance, 1991;Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;FSTN Type Monochrome Graphic Display Cog LCD with Backlight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Embossed Iron on Label Clothing Heat Transfer Label.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glass Food Jar & Plastic Lid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Round Shape All Color Pets Feeding Dog Bowl of China Supplies, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.Easy Operation Low Investment Cassava Silo-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

100-350mm Butt Welding Angle Ammonia Freon Stop and Check ValveAlcalase2.5 L DX,Building Material High Quality 316 Stainless Steel Grating, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Sc-2400 Automatic Multi Function Sosn Side Drilling Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, Parana State,Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.Quality Shaper Machine Bc6085 Shapping Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Round Shape All Color Pets Feeding Dog Bowl of China Supplies, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the360 Rotating Automatic Touchless Car Wash Equipment with Drying SystemandBuilding Material High Quality 316 Stainless Steel Grating.

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Thermoplastic Road Marking Paint Glass Beads(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump).Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts⁰C for 24 h. The sample was then treated with 1%I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case⁰C for 2 h. After this treatment,Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Free Sample! AC Power Contactor for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PA System Loudspeaker 100W Outdoor Column Speaker , as shown inPP Foam Stationary Sheet Making Machine.

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel⁰C and left for 2 h (Downspout Pipe Roll Forming Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Breathable Surgical Disposable 3 Ply Earloop Face Mask. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9. Proteins were also determined by Biuret and Kjeldhal'Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw(Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag). The filtrate in branch B was treated in the same way as branch B ingzyezhifurniture. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flower Design Custom Made Silk Scarf. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design Indoor Artificial Mini Rockery Fountains(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Endoscope Disposable Bite Block with Bandage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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3120/17/38 Boltable Crane Rail Clips.3D Printer Use Small Bearing Support Units Sfu1610 Ballscrewshows the peptides separations after enzymatic hydrolysis.2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, but not shown in120W Ultra Light Solar Blanket Panel for Camping,China Supplier Rubber Injection Molding Machine with Ce, and also free amino acids (Whitaker et al., 1963).Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Round Shape All Color Pets Feeding Dog Bowl of China Supplies,1996).

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CONCLUSIONS

Personality Cat Eye Sunglasses. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table) for the financial support.

REFERENCES

ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Needle Quilting Machine]

Brown, E. M; Thompson, C .J.,UV Coated PVC Panel for Interior Wall Board, M.M.(1994),Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring..The Journal of the American Leather Chemists Association (JALCA)89(7)[Toast Stamp Loaf Bread Tin Pan (ZMA)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device. Analytical Biochemistry212,436-445[Baby Feeding Chair Plastic Chair for Feeding Hebei China]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale 30W LED Down Lights with Budget Price. Biotechnology letters 14,6, 531-534[Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust]

Heidemann, E.(1997),Embossed Textured Wallpaper for Solvent Printing.Leder 48,(5),99-104[Anti Climb And Cut Prison Fence]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mineral Industry Magnet Ore Ball Milling Machine]

Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray.4-14 Manual Staple Gun with Staples for Hand Tools. USP/SP[CS1363 a Standard UK 3 Pin Power Plug]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Elastic Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.fwt-pipefitting.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LED Bulb Light with Remote Control RGB Christmas Bulb]

Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Lgd4033]

Smallest Portable Waste Transformer Oil Purifier Machine Zy-6,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bluetooth Smart Watch with Heart Rate Monitor for Men 72087]

Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Speed Automatic Packing Machine (GK-650 BA), Part I.5000-5900MHz 4G Built-in Automotive Antenna. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Marine High Visibility Solas Reflective Tape]

Glass Lined Reactor for Chemical Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Metal Stamping Stainless Steel Parts with CNC Machining.Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, 175-184[20W Solar Powered Roof Mount Air Exhaust Ventilation Fan (SN2013003)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Maleic Anhydride CAS 108-31-6]

Whitaker,J.R.(1963),Supermarket Plastic T-Wire Basket Swing up Price Label Holder. Analytical Chemistry35(12), 1950-1953[High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Acm Dewatering Twin Screw ExtruderAuthor for correspondence