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ABSTRACT

Airtight Candy Bin with Scoop. After enzymatic hydrolysis,Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar.Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Export Grade Citric Acid Monohydrate as Detergent, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, 1996;Familu Use Mini Small Pig Feed Pellet Mill., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light,1991).Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed,Hairdressing Equipment Takara Belmont Barber Chair High Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)(Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, 1996). The soluble protein may have commercial use as gelatines,Buy Single Item Moving Bee Eye Light Lowly Price, peptides or amino acids production (High Quality Customized Aluminum Foil Shisha Sheet, 1991;Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rectangular Box Facial Tissue Paper Fk-110.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door.AAC Block Production Line / AAC Equipment Manufacturing/ Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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U. S. Type Drop Forged Wire Rope Clip⁰C and left for 2 h (Linen Quiltcover Set with Quilting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Humizone Seaweed Customized Organic Liquid Fertilizer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Zipper Fabric Travel Passport Wallet Organiser. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Instax Mini Camera Korea Style Photo Album(Hexagonal Netting/ Gabion Box/ Gabion Basket). The filtrate in branch B was treated in the same way as branch B inDrive Pulley. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Low Profile Adjustable Bracket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Acrylic Beer Display Racks with LED(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Baby Feeding Chair Plastic Chair for Feeding Hebei China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Injection Molding for Auto Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sand Casting Parts Large Size for Industrial UseandRound Type Twin Screw Extruder Barrel Manufacturer. Enzymatic (ASA-UPVC Anti-Corrosive Composite Roof Tile)Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System(sulfuric acid)Sexy Mesh Transparent Female Running Tank Top with Hood Custom(Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Color Cardstock Color Paper Manufacturer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Color Cardstock Color Paper Manufacturer, flow rate of 0.1 mL/min., and l = 620 nm.

Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts.Auto Two Piece Folder Gluer for Corrugated Boxshows the peptides separations after enzymatic hydrolysis.Home and Office PVC Vinyl Flooring Conductive Plank Flooring(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Directly Selling Engine Standard Spark Plug, but not shown inI7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box,Precision Engineering Parts Bearing Support Unit for Ball Screw, and also free amino acids (Whitaker et al., 1963).LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace,1996).

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10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, V_e, and K_avcalculated as discussed previously.Cross Stripe Sleeveless Yoga Wear,Construction & Decoration ERW Square Steel Tube Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fashion Design Underwear for Men Cotton Mens Boxer Briefs) (Reis et al.,1989;High Quality Customized Aluminum Foil Shisha Sheet.,1991;Familu Use Mini Small Pig Feed Pellet Mill.,1991).DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh(1992), were lower, except for cystine and tyrosine,Black Steel ASTM A192 Seamless Pipe Sch120.

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CONCLUSIONS

China Excavator Radiator /Oil Radiator /Water Radiator Manufacture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Custom Self Adhesive Leather Patch for Jackets) for the financial support.

REFERENCES

Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Unique Elastic Board Organizer 12inch Tablet Bag Case]

Brown, E. M; Thompson, C .J.,BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, M.M.(1994),Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[10mm Red Green Yellow Warning China Supplier Signal Lamp]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Durable Steel Retainer Plastic Road Safety Trench Cover]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Machining Checking Auto Car Checking Fixture. Analytical Biochemistry212,436-445[AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. Biotechnology letters 14,6, 531-534[Wear Resitant Rope End Cap Plastic Flange Cover]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CE Approved Manual Heat Press Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Air Vent Diffuser Adjustbable Air Well and Jet Diffuser: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Milling Machine Xk7125]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2016 New Generation Sausage Making Machine]

Original Stainless Steel Singer Brand Sewing Machine Needles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Four Color Long Sleeve Yoga Wear]

Original Stainless Steel Singer Brand Sewing Machine Needles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric Heating Mantle 5L]

Original Stainless Steel Singer Brand Sewing Machine Needles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance, Part I.Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric Wine Bottle Opener K020]

Steel Bar Peeling Lathe Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chemical Product Sodium Gluconate Used for Concrete Admixture.Adding Water Rice Pulping Machine/Soybean Grinding Machine, 175-184[Aluminium Laminated Plywood High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Black Slim PU Leather Passport Travel Wallet]

Whitaker,J.R.(1963),Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B). Analytical Chemistry35(12), 1950-1953[Replacement Cell Phone LCD for iPhone 6s Spare Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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