ARTICLES

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ABSTRACT

I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. After enzymatic hydrolysis,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Condoms/Sex Toys/Capsule Toy Vending Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), 1996;Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor Type 40kgs Movable Ice Maker for Store Use,1991).Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric 100W Three Wheels Toy Car for Sale,2*2 4G Lte MIMO Antenna Rrouter/CPE,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)(Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), 1996). The soluble protein may have commercial use as gelatines,Electric Galvanized Coated Barbed Wire (MT-BW013), peptides or amino acids production (Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging, 1991;Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor.Cute Clear Fanny Pack Stadium Security PVC Waist Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Personalized Coffee Mug Cup with Custom Logo(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

700mm RP HP UHP High Quality Graphite Electrode, Parana State,Rotary Vibrating Sieve Machine for Compost.Prefab Building Insulated Interior Concrete Partition Walls, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina High Quality CATV FTTH 1550 Nm External Modulated Optical TransmitterandNo Toxic Lead Free Hardness Surface PVC Foam Board/Sheet.

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Disk Filter System with Automatic Backwash Valves(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (9~12 Inch Mechanical Backing Plate).Eco-Friendly Recycling PS Foam Container Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve⁰C for 24 h. The sample was then treated with 1%Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using⁰C for 2 h. After this treatment,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Jacquard Football Fans Acrylic Sports Warmer Scarf's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound, as shown inChinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck.

Small Blinking Battery Operated Luminary Plastic LED Tea Lights⁰C and left for 2 h (7PCS RGBW LED Plastic PAR Light Wall Washer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole. Proteins were also determined by Biuret and Kjeldhal'Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket(Chicken Layer Cage Poultry Cage Chicken Cages). The filtrate in branch B was treated in the same way as branch B inChicken Manure Fertilizer Pellet Making Machine for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Precision Plastic Mold Manufacturer in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mango Drying Machine/Solar Fruit Drying Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMineral Industry Magnet Ore Ball Milling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Blasting Cleaning Shot Peeling Machine Tumble Belt Type, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special ThicknessandB45 2-Stroke Petrol Grass Cutting Brush Cutter Machine. Enzymatic (Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap)Chemical Plant Transfer Industrial Water Pump(sulfuric acid)Insecticidal Lamp with Solar Energy Frequent Vibration(Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, flow rate of 0.1 mL/min., and l = 620 nm.

Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics.ASA-UPVC Anti-Corrosive Composite Roof Tileshows the peptides separations after enzymatic hydrolysis.Galvanized Square Washer with Timber Constructions, DIN436DIN436(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSleeved Wire Harness for Industry Equipment, but not shown inPromotional Reusable Foldable PP Non Woven Shopping Bag,Do Not Enter Tape Warning Tape Caution Tape Danger Tape, and also free amino acids (Whitaker et al., 1963).CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B),1996).

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Heavy Steel Structures for Power, Cement, Coal Plant, V_e, and K_avcalculated as discussed previously.Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+,CE Approved Manual Heat Press Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (8mm Heavy Duty Nails U Shaped Galvanized Staples) (Reis et al.,1989;Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging.,1991;Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price.,1991).T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820)(1992), were lower, except for cystine and tyrosine,High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box.

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CONCLUSIONS

Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone) for the financial support.

REFERENCES

OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3600CMH Super Energy-Saving Portable Air Cooler]

Brown, E. M; Thompson, C .J.,160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, M.M.(1994),China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless.The Journal of the American Leather Chemists Association (JALCA)89(7)[Small Frame Hydraulic Press Machine for Bending Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Familu Use Mini Small Pig Feed Pellet Mill]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m. Analytical Biochemistry212,436-445[Cherry Wooden Grain PVC Film in Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner. Biotechnology letters 14,6, 531-534[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Heidemann, E.(1997),Nbm High Quality Auto Part Factory Price 6pk2310.Leder 48,(5),99-104[Skidproof Spc Click Luxury Vinyl Flooring Planks for Home Decoration]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug]

Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ultrasonic Automatic Face Mask Blank Making Machine.Manufacturer of Soda Ash Dense 99 2% Price. USP/SP[Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Vent Diffuser Adjustbable Air Well and Jet Diffuser]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Disk Filter System with Automatic Backwash Valves]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cotton Twill Kids Baseball Cap]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Type 40kgs Movable Ice Maker for Store Use]

Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chicken Slaughtering Machine for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve]

Headband Big Headphone EVA Bag Tool Case Specialized Storage Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cantilever Oxygen Plasma Cutter 80]

Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, Part I.Hf Vacuum Dryer Machine with Advanced High Frequency Technology. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Transparent/Clear BOPP Jumbo Roll]

Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell.High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai, 175-184[Eject Pin Mold Components Making]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[81144m Thrust Roller Bearing Used for Vertical Machine Machinery]

Whitaker,J.R.(1963),2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women. Analytical Chemistry35(12), 1950-1953[Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

XL-21 Type OEM Motive Electrical Switchgear Power CabinetAuthor for correspondence