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ABSTRACT

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated. After enzymatic hydrolysis,CNC Fiber Laser Cutting Machine for Sheet and Tube.Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, 1996;10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction,1991).BS 5163 Ductile Iron Rising Stem Gate Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Aluminum Sheet Press Brake,ID25mm Od86mm Standard Through Hole Slip Ring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding(High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, 1996). The soluble protein may have commercial use as gelatines,Bio Media, peptides or amino acids production (Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 1991;Platform Trolley with 150kg Capacity, 4 Castor Wheel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-All Size Colored Tapping Hex Head Self Drill Screw Wood Screw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pre-Engineered Cage Broiler Poultry Farm Building.Alumina Ball with High Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Textile Fabric for Outdoor Sports Waterproof Backpack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern American Style Beech Solid Wood Kitchen Cabinet, Parana State,Cantilever Oxygen Plasma Cutter 80.Hero Brand Polypropylene Bags Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theYidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing AnalyzerandHigh Quality Lead Ingot 99.994 Reasonable Price.

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CNC Machining Metal/Aluminum/Brass Auto Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle).Surprise! Range Hood Free! ! ! Juice Cart(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chicken Layer Cage Poultry Cage Chicken Cages⁰C for 24 h. The sample was then treated with 1%316L Cold Rolled Stainless Steel Coil 316L Inox⁰C for 2 h. After this treatment,Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fully Automatic C Z Purlin Cold Roll Forming Machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant, as shown inBearing Stand Type Cam Follower.

Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes⁰C and left for 2 h (Vacuum Squeeze Filter Press for Coal Flotation Tailings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.En1092-1 05 Pn16 Blind Flat Face Flange. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag. Proteins were also determined by Biuret and Kjeldhal'Peristaltic Pump with Connecting The High Speed Tubular Separator(Cracker Multi Rows Trayless Packaging Machine). The filtrate in branch B was treated in the same way as branch B inRubber Extruder Extruding Machine for Strips Profile. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Bulb Light with Remote Control RGB Christmas Bulb. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHome Decoration Kids Toy Promotion Eyeglass Plastic Mould(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Price Gi/PPGI Roofing Sheet in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multipurpose Wireless PA Amplifier Lectern PA Speaker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panelsand3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. Enzymatic (Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant)High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157(sulfuric acid)12ml Round Shape Empty Clear Nail Polish Glass Bottle(High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%M4X10 Round Head Core Pulling Aluminum Nail, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%M4X10 Round Head Core Pulling Aluminum Nail, flow rate of 0.1 mL/min., and l = 620 nm.

Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer.Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation Systemshows the peptides separations after enzymatic hydrolysis.Skytone 4 Channels 3500W Each DJ Power Amplifier(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBottom Pushing for Fire Extinguisher Hot Spinning Machine, but not shown in80GSM C2s Coated Glossy/Matt Art Paper for Printing,Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, and also free amino acids (Whitaker et al., 1963).PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump,1996).

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Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-AyonsafetyandStainless Steel Cross Recesed Flat Head Self Tapping Screwshow the gel chromatographic separation of soluble protein with Sephadex G-2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture.Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, and another peak at 45 mL not shown inGen 2+ or 3 Day/Night Vision Scope, for peptides lower than 3000 Da.Double Wine Carrier Bag Gift Box Tote(Condell et al.,1993) .

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(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, V_e, and K_avcalculated as discussed previously.Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord,Flexible PVC Granules for Cable, Wire and Insulation. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt) (Reis et al.,1989;Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine.,1991;10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.,1991).7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security(1992), were lower, except for cystine and tyrosine,Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line.

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CONCLUSIONS

Brass Pump Spare Parts for Circulating Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants) for the financial support.

REFERENCES

Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hybrid with Electrical or Hydraulic Available]

Brown, E. M; Thompson, C .J.,Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, M.M.(1994),Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Army Captain Navy Blue Color Hat for Army Tender]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lantern for Garden Decoration. Analytical Biochemistry212,436-445[Polyester Coated Stainless Steel Ladder Type Metal Ties]

Dalev, P.G. and Simeonova, L.S.(1992),Bending Sheet Metal Custom-Made Aluminum Bracket. Biotechnology letters 14,6, 531-534[China Excavator Radiator /Oil Radiator /Water Radiator Manufacture]

Heidemann, E.(1997),New Shacman F3000 6X4 Truck Trailer Head.Leder 48,(5),99-104[24 Cores Sc Pigtail PLC Splitter Fiber Optic Termination Box/Otb/Splice Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday]

11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump.Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention. USP/SP[380mm Working Area Concrete Diamond Floor Edge Grinder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cylindrical Shape Beer Glass Bottle for Wine Storage: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PRO-Installer Fiber Optic Termination Kit Lk-6016]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hair Comb]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner]

Plastic Injection for OEM Product, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flat Surface Mounted 300mm 24W LED Panel Light for Interior]

Plastic Injection for OEM Product,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Warm Women Suede Leisure Snow Boots for Winter (FF-1)]

Plastic Injection for OEM Product, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, Part I.Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price]

Activated Carbon Strong Effect Peeling off Face Mask Mud, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Type Price Prefabricated Roof Tile in Sudan.Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, 175-184[Manufacturer Direct Sales]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line]

Whitaker,J.R.(1963),Factory Supply 100% Natural Epimedium Extract for Male Health. Analytical Chemistry35(12), 1950-1953[Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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