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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. After enzymatic hydrolysis,High Quality Sheet Metal Bending Roll Forming Machine.Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine;Frame Type Rubber Hydraulic Press Machine; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Kitchen Metal Dish Storage Rack (JP-SC984C), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, 1996;AVR Se350 Automatic Voltage Regulator for Marathon Alternator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage,1991).Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Strength Rigid PVC Plastic Foam Board for FRP,Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall(0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, 1996). The soluble protein may have commercial use as gelatines,Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, peptides or amino acids production (Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould, 1991;LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10ADSS Newest Ultra Therapy Skin Tightening 3D Hifu.Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


High Quality Ceramic Chrome Hybrid Bearing OilAlcalase2.5 L DX,Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Digger Arm Small Wheel Loader (HQ908) with CE(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, Parana State,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper.Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the9~12 Inch Mechanical Backing PlateandChinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck.

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Water Based Emulsion Paint Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Chicken Layer Cage Poultry Cage Chicken Cages).Cnm Single Strand Anchor Coupler(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp0C for 24 h. The sample was then treated with 1%18X16mesh Fiberglass Window Screen Insect Screen0C for 2 h. After this treatment,Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sterile Dsiposable Syringe for Dental 12ml's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Foldable Bed Military Cot for Camping, as shown inWholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness.

P5 960*960mm LED Display Screen SMD Indoor /Outdoor0C and left for 2 h (ASA-UPVC Anti-Corrosive Composite Roof Tile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 New Advertising Inflatables Air Walking Backpack Ball. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Side Pack Manual Gas Engine Power Brush Cutter. Proteins were also determined by Biuret and Kjeldhal'Punching Bending Welding Steel Work Fabrication(Sleeved Wire Harness for Industry Equipment). The filtrate in branch B was treated in the same way as branch B in1000kg Machine Roomless Passenger Lift Elevator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav1 Ton Chain Pulley Block with Geared Trolley(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Plastic Part Hardware Plastic Injection Mold0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IEC 62196-2 Type 2 EV Charging 32A Male Plug, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Promotional Reusable Foldable PP Non Woven Shopping BagandPTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter. Enzymatic (180W 1850mm*80mm Reci Laser Tube)OEM Factory Balloon Padding Textile Machinery(sulfuric acid)NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy(0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), flow rate of 0.1 mL/min., and l = 620 nm.

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.PVC Cross Joint Pipe Fittings Equal Pipe Crossshows the peptides separations after enzymatic hydrolysis.Outdoor Concert Stage Equipment Aluminum Stage Portable Stage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, but not shown inWholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers,Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, and also free amino acids (Whitaker et al., 1963).22*30mm 12SMD 5050 LED Reading Light for Car. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming,1996).

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Modern American Style Beech Solid Wood Kitchen Cabinetand8mm Heavy Duty Nails U Shaped Galvanized Staplesshow the gel chromatographic separation of soluble protein with Sephadex G-SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital.Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, and another peak at 45 mL not shown in6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower, for peptides lower than 3000 Da.Outdoor Cheap Portable Stage Aluminum Truss Stage(Condell et al.,1993) .

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One Arm Motorized Multifunction Hospital Equipment for China Supply, Ve, and Kavcalculated as discussed previously.Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts,P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Small Frame Hydraulic Press Machine for Bending Price) (Reis et al.,1989;Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould.,1991;AVR Se350 Automatic Voltage Regulator for Marathon Alternator.,1991).Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine(1992), were lower, except for cystine and tyrosine,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.

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Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder) for the financial support.


Printed Wristband Cotton Wristband RFID Woven Wristband, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale CE Approved Jp Jianping Brushless Rotor Dynamic Balancing]

Brown, E. M; Thompson, C .J.,High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, M.M.(1994),Dolphin Designed Handheld Massager (LC-889).The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Real Leather Wallet Mens Designer Leather Wallets. Analytical Biochemistry212,436-445[Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers]

Dalev, P.G. and Simeonova, L.S.(1992),ASTM A240 321 Stainless Steel Sheet Price Per Kg. Biotechnology letters 14,6, 531-534[Air Vent Diffuser Adjustbable Air Well and Jet Diffuser]

Heidemann, E.(1997),V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor.Leder 48,(5),99-104[HD Video Wall P3.91 LED Panel Display Indoor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disk Filter System with Automatic Backwash Valves]

Ductile Iron Standard Brackets Pipe Hanger, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Promotional Plain Silver Empty Hinged Rectangular Tin Box.Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling. USP/SP[Outdoor Type 40kgs Movable Ice Maker for Store Use]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cantilever Oxygen Plasma Cutter 80]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wood Plank Laminate Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Transparent/Clear BOPP Jumbo Roll]

Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[81144m Thrust Roller Bearing Used for Vertical Machine Machinery]

Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears]

Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), Part I.Cantilever Oxygen Plasma Cutter 80. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[XL-21 Type OEM Motive Electrical Switchgear Power Cabinet]

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Fiber Laser Cutting Machine for Sheet and Tube.Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, 175-184[Hot Rolled special alloy Die Steel Round Bar 1.2316 AISI420 4Cr16]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hairdressing Equipment Takara Belmont Barber Chair High Quality]

Whitaker,J.R.(1963),Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security. Analytical Chemistry35(12), 1950-1953[Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sand Castings by Gray Iron MaterialAuthor for correspondence

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