ARTICLES

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ABSTRACT

St1000 Fire Resistant Steel Cord Rubber Belt. After enzymatic hydrolysis,Four Color Long Sleeve Yoga Wear.Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, 1996;Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (White HPL 600*600mm Coffee Table,1991).Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Price 6000 Series Industrial Extruded Aluminum Tube,WPC Decking Profile Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile(XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, 1996). The soluble protein may have commercial use as gelatines,CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, peptides or amino acids production (Party Mask LED Fiber Dance Princess Feather Mask, 1991;80GSM C2s Coated Glossy/Matt Art Paper for Printing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reusable Silicone Straight Straws.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Easy Foldable Kids Walking Chair Toys Baby Walker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Yellow Hazardous Spill Response Chemical Spill Kits.Anhui Hydraulic Sheet Metal Bending Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:304 Stainless Steel Standard V-Band Clamps for Turbo(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, Parana State,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt.Eco-Friendly Aluminum Mechanical Part, CNC Machining, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the4 Styles Blue Glass Crystal Butt Beads Spiral PlugandDecoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum.

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Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Transparent Color Stretch Wrap Film Manual Operate Wrap Film).Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine⁰C for 24 h. The sample was then treated with 1%Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric⁰C for 2 h. After this treatment,Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ductile Iron Flanged Swing Check Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ozonated Water for Vegetables and Fruits Washing Ozone Generator, as shown inMotor Bearing Automobile Bearing Self-Aligning Ball Bearing.

Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose⁰C and left for 2 h (Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Day and Night Zebra Blind Fabric Z1602. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. Proteins were also determined by Biuret and Kjeldhal'Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)(Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter). The filtrate in branch B was treated in the same way as branch B inFast Speed Fat Tire Bike Ele⪞ Tri⪞ ⪞ Ity⪞ O⪞ O. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Impact Sound Isolation Wall Clip.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGerman Technology Automatic Stacking System in Red Brick Automatic Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.13.56 MHz Fudan FM11RF08 Chip Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale 30W LED Down Lights with Budget PriceandSnooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. Enzymatic (100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap)Square Premium Glass Perfume Bottle 100ml(sulfuric acid)Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds(XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, flow rate of 0.1 mL/min., and l = 620 nm.

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep.Iron Cast Brake Drums for Trucksshows the peptides separations after enzymatic hydrolysis.Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTrendy High Quality 925 Stering Silver Tennis Glod Charm, but not shown inTropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper,High Quality Inflatable PVC Flocking Health Bed, and also free amino acids (Whitaker et al., 1963).Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (XL-21 Type OEM Motive Electrical Switchgear Power Cabinet,1996).

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Underground Garden 6W High Power Outdoor LED Lawn Light, V_e, and K_avcalculated as discussed previously.Stamping Metal Parts Manufacturer Sheet Parts Fabrication,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light) (Reis et al.,1989;Party Mask LED Fiber Dance Princess Feather Mask.,1991;Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient.,1991).3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY(1992), were lower, except for cystine and tyrosine,Hexagonal Netting/ Gabion Box/ Gabion Basket.

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CONCLUSIONS

Different Thickness High Quality Aluminum Foil for Glass Bottle Lid. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light) for the financial support.

REFERENCES

Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 Fashion Hoop Earrings Hexagon Women Wholesale Clip on Earrings]

Brown, E. M; Thompson, C .J.,Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, M.M.(1994),Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fine Bubble Diffuser for Air Purifier]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. Analytical Biochemistry212,436-445[Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Ozone Disinfection Machine Ozone Generator. Biotechnology letters 14,6, 531-534[Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings]

Heidemann, E.(1997),Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube.Leder 48,(5),99-104[Tungsten Carbide Cold Heading Dies for Screws and Nuts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Copper]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[One Arm Motorized Multifunction Hospital Equipment for China Supply]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bobby Flay Websites Restaurant Martha Stewart Designer Tray: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Manufacturer for Logistics Agent In Tianjin -]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[British Standard 2 Gang 1 Way Wall Light Switch]

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Reusable Silicone Straight Straws]

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Leather Shoes Craft Laser Cutting Machine]

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Diameter 2.68m Paragraphed Autoclave for AAC Bricks, Part I.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1 Inch NPT Screwed Globe Valve]

Travelling Weekender Mens Canvas Duffle Gym Sports Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box.China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 175-184[450mm Marble Cutting Diamond Saw Blade]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar]

Whitaker,J.R.(1963),Best Selling Household Living Room Mat Chenille Carpets. Analytical Chemistry35(12), 1950-1953[Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Machining 6601 Aluminum Parts with Anodized AutomoblileAuthor for correspondence