ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)

ABSTRACT

Garlic Red Mesh Bag. After enzymatic hydrolysis,Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder.Ground Finish Molybdenum Tzm Rod. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Transparent/Clear BOPP Jumbo Roll;Yashi 19 270degree Pyramid Hologram Display Showcase Attractive; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chenille Microfiber Mop Head, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Waterproof Pizza Sign Large Advertising Signboard, 1996;PVC Shrink Sleeve Label for Drinking Water Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4,1991).Galvanized Square Wire Mesh, Metal Window Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory,2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Customized Stainless Steel Parts by Deep Drawing(Custom Waterproof Pizza Sign Large Advertising Signboard, 1996). The soluble protein may have commercial use as gelatines,Children Bear English Story Book Printing, peptides or amino acids production (Flexible Board FPC PCB Camera Module Rigid Flex PCB, 1991;High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cherry Wooden Grain PVC Film in Furniture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Waterproof Pizza Sign Large Advertising Signboard, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44).Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Linen Quiltcover Set with Quilting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bto 22 Electric Galvanized Razor Concertina Wire for Military, Parana State,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144).Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Waterproof Pizza Sign Large Advertising Signboard, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable ScooterandLow Power Consumption Laser Foam Vertical Die Cutting Machine.

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Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning).Hot Selling Military Large Event Marquee Tent Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High-Quality Pre-Seasoned Cast Iron Wok⁰C for 24 h. The sample was then treated with 1%2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle⁰C for 2 h. After this treatment,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Marine High Visibility Solas Reflective Tape's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, as shown inWholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars.

Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)⁰C and left for 2 h (China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Magic Tape Hook and Loop Fasteners. Proteins were also determined by Biuret and Kjeldhal'Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool(Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring). The filtrate in branch B was treated in the same way as branch B inm.sheenlandglass.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStarch Doy Bag Packaging Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Soft PVC Rubber Keychains for Promation Gift⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark.Fire Extinguisher UV Curing Machine Cylinder Drying Machineshows the peptides separations after enzymatic hydrolysis.Custom Grey Full-Zip Cotton Mens Hoodies(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in700mm RP HP UHP High Quality Graphite Electrode, but not shown inBetaine 98% Betaina Feed Grade,Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, and also free amino acids (Whitaker et al., 1963).7inch Industrial Tablet Computer Naked Resistance Touch Screen. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Waterproof Pizza Sign Large Advertising Signboard,1996).

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CONCLUSIONS

Premium Grade Rubber Cloth Duct Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Teerte Square Flat Head Self Tapping Screw) for the financial support.

REFERENCES

Sud1000h Poly Welder Butt Fusion Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Black And White Marble Mosaic Floor Tiles High Quality Supplier]

Brown, E. M; Thompson, C .J.,Customized Anti-Rust Torsion Spring, M.M.(1994),Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polished Tzm Molybdenum Alloy Plate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Analytical Biochemistry212,436-445[180W 1850mm*80mm Reci Laser Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. Biotechnology letters 14,6, 531-534[Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights]

Heidemann, E.(1997),OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic.Leder 48,(5),99-104[9 Pin Circular Connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine]

New Condition Hot Sale Automatic Animal Feed Cooler Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PP Woven Bag, Rice, Beans, Plastic Bag.China Professional Mold Factory Plastic Injection Mould Maker. USP/SP[Food Chopper]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Dioxide Purification]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover]

Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Carbon Steel Wire Hose Hydraulic Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output]

Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale]

Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:24k Gold Whitening Anti-Spot Mask, Part I.Clay Tiles Making Machine with Latest Technology. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PVC Ceiling Wall Pannelling]

DIN Standard Gratings/Gavlanized Steel Grating, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),50mm Magnetic River Water Sewage Flow Meter for Acid.PE Biodegradable Sheet Product Line/Single Screw Extruder, 175-184[Zinc Plated Hex Flange Nut]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Furniture Grade E1 Glue Plywood with Competitive Price]

Whitaker,J.R.(1963),27L/Min Water Exchanger Heat Pump Mold Temperature Machine. Analytical Chemistry35(12), 1950-1953[Round Type Twin Screw Extruder Barrel Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)Author for correspondence