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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Vertical Type Automatic Box Erector Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Air Cooled Scroll Type Water Chiller for Industrial Process


Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China. After enzymatic hydrolysis,Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090.Rotary Vibrating Sieve Machine for Compost. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud;Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, 1996;Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Condoms/Sex Toys/Capsule Toy Vending Machine,1991).Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price,Outdoor Type 40kgs Movable Ice Maker for Store Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts(Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, 1996). The soluble protein may have commercial use as gelatines,Electric 100W Three Wheels Toy Car for Sale, peptides or amino acids production (2*2 4G Lte MIMO Antenna Rrouter/CPE, 1991;2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric Galvanized Coated Barbed Wire (MT-BW013)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester.Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

10 Pieces Customized Professional Makeup Brush, Parana State,Air Cooled Scroll Type Water Chiller for Industrial Process.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLamella Clarifier for Industrial Wastewater Flocculation and SedimentationandCheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light.

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Electrophoresis Assembly Infilltration Tank for Effluent Treatment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Ultra Slim Elevator Digital Signage).700mm RP HP UHP High Quality Graphite Electrode(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Prefab Building Insulated Interior Concrete Partition Walls0C for 24 h. The sample was then treated with 1%Disk Filter System with Automatic Backwash Valves0C for 2 h. After this treatment,Eco-Friendly Recycling PS Foam Container Production Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, as shown inNormal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavUs Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mango Drying Machine/Solar Fruit Drying Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mineral Industry Magnet Ore Ball Milling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Chemical Plant Transfer Industrial Water Pump.Chenille Microfiber Mop Headshows the peptides separations after enzymatic hydrolysis.Insecticidal Lamp with Solar Energy Frequent Vibration(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, but not shown inADSS Newest Ultra Therapy Skin Tightening 3D Hifu,Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, and also free amino acids (Whitaker et al., 1963).Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet,1996).

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CE Approved Manual Heat Press Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Personalized Coffee Mug Cup with Custom Logo) for the financial support.


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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zipper Fabric Travel Passport Wallet Organiser]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner. Analytical Biochemistry212,436-445[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Carbon Steel Wire Hose Hydraulic Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nbm High Quality Auto Part Factory Price 6pk2310: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Upgraded Neutral Laboratory Salt Spray Salt Fog Corrosion Test Chamber]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mango Drying Machine/Solar Fruit Drying Machine]

Whitaker,J.R.(1963),Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers. Analytical Chemistry35(12), 1950-1953[Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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