ARTICLES

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ABSTRACT

Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube. After enzymatic hydrolysis,Condoms/Sex Toys/Capsule Toy Vending Machine.Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electric 100W Three Wheels Toy Car for Sale, 1996;2*2 4G Lte MIMO Antenna Rrouter/CPE., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′),1991).Electric Galvanized Coated Barbed Wire (MT-BW013). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging,Slotter Sheet Metal V Grooving Machine From Professional Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester(Electric 100W Three Wheels Toy Car for Sale, 1996). The soluble protein may have commercial use as gelatines,Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool, peptides or amino acids production (Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter, 1991;China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cute Clear Fanny Pack Stadium Security PVC Waist Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Personalized Coffee Mug Cup with Custom Logo.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electric 100W Three Wheels Toy Car for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10 Pieces Customized Professional Makeup Brush.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electrophoresis Assembly Infilltration Tank for Effluent Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Precision Plastic Mold Manufacturer in China⁰C and left for 2 h (300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mango Drying Machine/Solar Fruit Drying Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mineral Industry Magnet Ore Ball Milling Machine. Proteins were also determined by Biuret and Kjeldhal'DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut(5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap). The filtrate in branch B was treated in the same way as branch B inPVC Arrow Safety Reflective Sticker Reflective Warning Tape. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlasting Cleaning Shot Peeling Machine Tumble Belt Type(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chemical Plant Transfer Industrial Water Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Insecticidal Lamp with Solar Energy Frequent Vibration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws.Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminumshows the peptides separations after enzymatic hydrolysis.Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSmallest Portable Waste Transformer Oil Purifier Machine Zy-6, but not shown inTransparent Color Stretch Wrap Film Manual Operate Wrap Film,Good Quality PVC Plastic Ceiling for Wall Panel, and also free amino acids (Whitaker et al., 1963).Heavy Steel Structures for Power, Cement, Coal Plant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electric 100W Three Wheels Toy Car for Sale,1996).

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CONCLUSIONS

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disk Filter System with Automatic Backwash Valves) for the financial support.

REFERENCES

600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rotating flying chair]

Brown, E. M; Thompson, C .J.,Nbm High Quality Auto Part Factory Price 6pk2310, M.M.(1994),Ultrasonic Automatic Face Mask Blank Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer of Soda Ash Dense 99 2% Price. Analytical Biochemistry212,436-445[China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless]

Dalev, P.G. and Simeonova, L.S.(1992),Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. Biotechnology letters 14,6, 531-534[Automotive Acrylic Wholesale Masking Tape Factory Price]

Heidemann, E.(1997),Headband Big Headphone EVA Bag Tool Case Specialized Storage Box.Leder 48,(5),99-104[20x Optical Zoom Ip Camera]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Waterproof Pizza Sign Large Advertising Signboard]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hf Vacuum Dryer Machine with Advanced High Frequency Technology: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fine Bubble Diffuser for Air Purifier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Brand Mango Energy Save Commerical Guangzhou Heat Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace]

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Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings]

Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai, Part I.2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Copper]

Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Oak Laminate Grey Floating Cork Vinyl Plank Flooring.China Manufacturer Customized Microwave Ring Ferrite Magnet, 175-184[Portable Foam Stainless Steel Hand Exerciser Spring-grip Hand Grip Strengthener]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Customized Stainless Steel Parts by Deep Drawing]

Whitaker,J.R.(1963),Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish. Analytical Chemistry35(12), 1950-1953[One Arm Motorized Multifunction Hospital Equipment for China Supply]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-FishAuthor for correspondence