ARTICLES

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ABSTRACT

500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. After enzymatic hydrolysis,Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings.Polyester / Polypropylene Woven Geotextile for Road Construction. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Granite Stone Jaw Crusher of Mining Machine;Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, 1996;Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale All Kinds Cheap Plush Animal Baby Slippers,1991).Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane,Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress(The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, 1996). The soluble protein may have commercial use as gelatines,Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale, peptides or amino acids production (Taiding Custom Tank Sheet Metal Fabrication, 1991;Airtight Candy Bin with Scoop, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3 Layer Round Tea Tin with Air Tight Lid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China PA System Power Amplifier with Enough Rated Power.Export Grade Citric Acid Monohydrate as Detergent-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Buy Single Item Moving Bee Eye Light Lowly Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Diameter 2.68m Paragraphed Autoclave for AAC Bricks).High Quality Customized Aluminum Foil Shisha Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank⁰C for 24 h. The sample was then treated with 1%Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment⁰C for 2 h. After this treatment,Rectangular Box Facial Tissue Paper Fk-110. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, as shown inMovable Folding Knuckle Boom Hydraulic Truck-Mounted Crane.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av380mm Working Area Concrete Diamond Floor Edge Grinder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Discussion and Interpretation System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Humizone Seaweed Customized Organic Liquid Fertilizer.Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinetsshows the peptides separations after enzymatic hydrolysis.Zipper Fabric Travel Passport Wallet Organiser(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair, but not shown inPE Biodegradable Sheet Product Line/Single Screw Extruder,Hot Sale Instax Mini Camera Korea Style Photo Album, and also free amino acids (Whitaker et al., 1963).Aluminum Low Profile Adjustable Bracket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine,1996).

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Baby Feeding Chair Plastic Chair for Feeding Hebei China, V_e, and K_avcalculated as discussed previously.Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle,Plastic Injection Molding for Auto Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( for Patio Garden Backyard (Warm white)) (Reis et al.,1989;Taiding Custom Tank Sheet Metal Fabrication.,1991;Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag.,1991).Round Type Twin Screw Extruder Barrel Manufacturer(1992), were lower, except for cystine and tyrosine,ASA-UPVC Anti-Corrosive Composite Roof Tile.

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CONCLUSIONS

Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer) for the financial support.

REFERENCES

Sexy Mesh Transparent Female Running Tank Top with Hood Custom, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6 Way Extension Cord Socket With Switch]

Brown, E. M; Thompson, C .J.,Color Cardstock Color Paper Manufacturer, M.M.(1994),Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Platform Trolley with 150kg Capacity]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Custom Self Adhesive Leather Patch for Jackets]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Home and Office PVC Vinyl Flooring Conductive Plank Flooring. Analytical Biochemistry212,436-445[Outsole Trimming Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Precision Engineering Parts Bearing Support Unit for Ball Screw. Biotechnology letters 14,6, 531-534[Airtight Candy Bin with Scoop]

Heidemann, E.(1997),LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection.Leder 48,(5),99-104[Fashion Watches New Design Bracelet Watches Ladies Women Wrist Watches]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Spare Parts Ignition Coil Fp85-18-100c]

Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man.Silicone Cake Mold Sinle Cupcake DIY. USP/SP[The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Side Pack Manual Gas Engine Power Brush Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stationery PP Clipboard With 7 Inch Scale Ruler]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Etc Tube Solar Water Heater]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines]

10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cross Stripe Sleeveless Yoga Wear-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Day and Night Zebra Blind Fabric Z1602]

10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter]

10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Construction & Decoration ERW Square Steel Tube Manufacturer, Part I.DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)]

Black Steel ASTM A192 Seamless Pipe Sch120, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Excavator Radiator /Oil Radiator /Water Radiator Manufacture.Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, 175-184[Neoprene Wrist Support]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Effluent Treatment Ferrous Sulphate Heptahydrate 98%]

Whitaker,J.R.(1963),BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film. Analytical Chemistry35(12), 1950-1953[Vacuum Stainless Steel Casting Products]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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