Promotional Plain Silver Empty Hinged Rectangular Tin Box

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

High Quality Cacao Bean Grinder Cocoa Butter Making Machine

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

1650mm*80mm Stable Power Laser Tube


Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile

The Black Carbon Galvanized Steel Angle BarEdivaldo E.GarciaI; Cláudia C.M.Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for RoofingI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiTablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-UCP Color Army Camouflage Uniform Army Military Uniform Suit. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,0.5 Gallons Cool Mist Ultrasonic Humidifier


Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. After enzymatic hydrolysis,Sanjing PVC Lining Layflat Fire Hose.Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light;Automatic CNC Glass Cutting Machine Glass Cutting Table; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996;Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pet PP Rope and Broom Wire Drawing Machine,1991).14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PTFE Seat Wafer Butterfly Valve Pn16,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults(Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996). The soluble protein may have commercial use as gelatines,Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, peptides or amino acids production (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1991;Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plain Makeup Bag Canvas Cosmetic Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple.Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Wholesale Qualited Polyester Padding Men Jacket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash CamAlcalase2.5 L DX,Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, Parana State,0.5 Gallons Cool Mist Ultrasonic Humidifier.River Water Cleaning Sludge Sediment Machine Lamella Clarifier, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHero Brand Polypropylene Bags MachineandBobby Flay Websites Restaurant Martha Stewart Designer Tray.

Export Grade Citric Acid Monohydrate as Detergent

Kick Folding Scooter

Polyester Coated Stainless Steel Ladder Type Metal Ties(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (1325 CNC Engraving Router).2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder0C for 24 h. The sample was then treated with 1%Copper Laminated Steel Strip T2/C110000C for 2 h. After this treatment,Halal Empty Gelatin Capsule for Medicine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gym Fitness Exercise Sporting Body Building Bicep Press, as shown inCustom LCD Screen Video Box for Package.

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck0C and left for 2 h (50kg Unmanned Two-Way Magnetic Tape Guided Agv). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. Proteins were also determined by Biuret and Kjeldhal'300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile(Boned Polyester Woven Faux Suede Sofa Fabric). The filtrate in branch B was treated in the same way as branch B inCar Parts Clutch Master Cylinder for Navara 30610-Eb300. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavFurniture Drawer Factory 2 Drawer Dresser Bedside Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA FreeandElectrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Enzymatic (Plastic Food Box Container Covers by Injection Mould Mold Tool)304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment(sulfuric acid)Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Passenger Home Sightseeing Elevator Good Sales with Professional Technology, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Passenger Home Sightseeing Elevator Good Sales with Professional Technology, flow rate of 0.1 mL/min., and l = 620 nm.

Vacuum Stainless Steel Casting Products.Wholesale China Merchandise 12V Horizontal Mini Camping Fridgeshows the peptides separations after enzymatic hydrolysis.Chicken Farming Materials Poultry Battery Cages Layer Battery Cage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDouble Wine Carrier Bag Gift Box Tote, but not shown inOur Perforated Aluminum & Solid Panel for New Office Wall Cladding,1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, and also free amino acids (Whitaker et al., 1963).I-Tech 12000HD 2u Space Digital Professional Power Amplifier. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken,1996).

2019 New Type of Household UF Water Filter with Top Quality and Best Price

Easy Foldable Kids Walking Chair Toys Baby Walker

220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain HoistandChina Manufacturer Customized Microwave Ring Ferrite Magnetshow the gel chromatographic separation of soluble protein with Sephadex G-Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer.High Quality Silicone Reusable Food Storage Bag China Manufacturer, and another peak at 45 mL not shown inCost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal, for peptides lower than 3000 Da.Polyurethane Gasket Foam Seal Dispensing Machine(Condell et al.,1993) .

Melamine Furniture

Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending

Imee Print Customized Printing Agenda Printing Nb21, Ve, and Kavcalculated as discussed previously.Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml,High Performance Multifunctional Crawler Mini Skid Steer Loader. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign) (Reis et al.,1989;Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01).,1991;Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.,1991).Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar(1992), were lower, except for cystine and tyrosine,Chunke Portable UV Water Sterilizer for Water Purifier 150t.

Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria

Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer


Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration) for the financial support.


Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Pressure PVC Pipe List Plastic Pipe]

Brown, E. M; Thompson, C .J.,Xjm Series Rubber Extruding Machine, M.M.(1994),Stop Dog From Jumping or Climbing Your Chain Link Fence..The Journal of the American Leather Chemists Association (JALCA)89(7)[Square Plastic PP Geocomposite Drainage Blind Ditch/Drain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SGS Direct Thermal Label for Supermarket. Analytical Biochemistry212,436-445[OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating]

Dalev, P.G. and Simeonova, L.S.(1992),Water Filter Polyester Non-Woven Fabric. Biotechnology letters 14,6, 531-534[Adding Water Rice Pulping Machine/Soybean Grinding Machine]

Heidemann, E.(1997),Industry Pet Recycling Systems, Industry Pet Recycling Line.Leder 48,(5),99-104[yuyancable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)]

Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sublimation Polyester Sports Tshirt for Adult.Heavy Duty Marquee Advertising Pop up Tent. USP/SP[Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3W LED Explosion Proof Emergency Exit Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Long Sleeve Compression Shirt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Arrival Wooden Kids Cleaning Set Toy for Education W10d251]

Fire Extinguisher UV Curing Machine Cylinder Drying Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FRP GRP Fiber Reinforced Plastic Fiberglass Gratings]

Fire Extinguisher UV Curing Machine Cylinder Drying Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gym Fitness Exercise Sporting Body Building Bicep Press]

Fire Extinguisher UV Curing Machine Cylinder Drying Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Energy-Saving Biogas Double Stove for Sale, Part I.Valve Bag Dry Mortar Bagging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)]

High Quality 500m 100lb Super Strong Braided Fishing Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, 175-184[Metal Steel Fiber Laser Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ New Shape Ceramic Mug]

Whitaker,J.R.(1963),Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. Analytical Chemistry35(12), 1950-1953[Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Men Fashion Plaid Design Polyester Ties (WH24)Author for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under aPeugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell