ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-UCP Color Army Camouflage Uniform Army Military Uniform Suit. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,0.5 Gallons Cool Mist Ultrasonic Humidifier

ABSTRACT

Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. After enzymatic hydrolysis,Sanjing PVC Lining Layflat Fire Hose.Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light;Automatic CNC Glass Cutting Machine Glass Cutting Table; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996;Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pet PP Rope and Broom Wire Drawing Machine,1991).14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PTFE Seat Wafer Butterfly Valve Pn16,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults(Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996). The soluble protein may have commercial use as gelatines,Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, peptides or amino acids production (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1991;Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plain Makeup Bag Canvas Cosmetic Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple.Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Wholesale Qualited Polyester Padding Men Jacket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, Parana State,0.5 Gallons Cool Mist Ultrasonic Humidifier.River Water Cleaning Sludge Sediment Machine Lamella Clarifier, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHero Brand Polypropylene Bags MachineandBobby Flay Websites Restaurant Martha Stewart Designer Tray.

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Polyester Coated Stainless Steel Ladder Type Metal Ties(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1325 CNC Engraving Router).2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder⁰C for 24 h. The sample was then treated with 1%Copper Laminated Steel Strip T2/C11000⁰C for 2 h. After this treatment,Halal Empty Gelatin Capsule for Medicine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gym Fitness Exercise Sporting Body Building Bicep Press, as shown inCustom LCD Screen Video Box for Package.

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck⁰C and left for 2 h (50kg Unmanned Two-Way Magnetic Tape Guided Agv). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. Proteins were also determined by Biuret and Kjeldhal'300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile(Boned Polyester Woven Faux Suede Sofa Fabric). The filtrate in branch B was treated in the same way as branch B inCar Parts Clutch Master Cylinder for Navara 30610-Eb300. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFurniture Drawer Factory 2 Drawer Dresser Bedside Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Vacuum Stainless Steel Casting Products.Wholesale China Merchandise 12V Horizontal Mini Camping Fridgeshows the peptides separations after enzymatic hydrolysis.Chicken Farming Materials Poultry Battery Cages Layer Battery Cage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDouble Wine Carrier Bag Gift Box Tote, but not shown inOur Perforated Aluminum & Solid Panel for New Office Wall Cladding,1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, and also free amino acids (Whitaker et al., 1963).I-Tech 12000HD 2u Space Digital Professional Power Amplifier. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken,1996).

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CONCLUSIONS

Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration) for the financial support.

REFERENCES

Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Pressure PVC Pipe List Plastic Pipe]

Brown, E. M; Thompson, C .J.,Xjm Series Rubber Extruding Machine, M.M.(1994),Stop Dog From Jumping or Climbing Your Chain Link Fence..The Journal of the American Leather Chemists Association (JALCA)89(7)[Square Plastic PP Geocomposite Drainage Blind Ditch/Drain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SGS Direct Thermal Label for Supermarket. Analytical Biochemistry212,436-445[OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating]

Dalev, P.G. and Simeonova, L.S.(1992),Water Filter Polyester Non-Woven Fabric. Biotechnology letters 14,6, 531-534[Adding Water Rice Pulping Machine/Soybean Grinding Machine]

Heidemann, E.(1997),Industry Pet Recycling Systems, Industry Pet Recycling Line.Leder 48,(5),99-104[yuyancable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)]

Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sublimation Polyester Sports Tshirt for Adult.Heavy Duty Marquee Advertising Pop up Tent. USP/SP[Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3W LED Explosion Proof Emergency Exit Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Long Sleeve Compression Shirt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.lowcled.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Arrival Wooden Kids Cleaning Set Toy for Education W10d251]

Fire Extinguisher UV Curing Machine Cylinder Drying Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FRP GRP Fiber Reinforced Plastic Fiberglass Gratings]

Fire Extinguisher UV Curing Machine Cylinder Drying Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gym Fitness Exercise Sporting Body Building Bicep Press]

Fire Extinguisher UV Curing Machine Cylinder Drying Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Energy-Saving Biogas Double Stove for Sale, Part I.Valve Bag Dry Mortar Bagging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)]

High Quality 500m 100lb Super Strong Braided Fishing Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, 175-184[Metal Steel Fiber Laser Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ New Shape Ceramic Mug]

Whitaker,J.R.(1963),Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. Analytical Chemistry35(12), 1950-1953[Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Men Fashion Plaid Design Polyester Ties (WH24)Author for correspondence