ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring

ABSTRACT

Customized Hot Forging Agricultural Spare Parts. After enzymatic hydrolysis,16mm 12 Strand UHMWPE Synthetic Fiber Rope.High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Vertical Type Caseformer Machine WJ-LKX-15;Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, 1996;Agriculture Large Span Commerical Steel Frame Greenhouse., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat,1991).14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ss400 Corner Metal Sheet Bar Galvanized Angle Iron,High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer(0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, 1996). The soluble protein may have commercial use as gelatines,Chinese Jbcz Handy Operation Threading Machine and Coupler, peptides or amino acids production (Promotional Reusable Foldable PP Non Woven Shopping Bag, 1991;Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price Printing Doy Pack Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price.High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Engine Parts Rubber High Quality Timing Belt 111ru25(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, Parana State,Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.Bottom Pushing for Fire Extinguisher Hot Spinning Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEasy Foldable Kids Walking Chair Toys Baby Walkerand220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist.

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Fish Scales Texture Fashion Leather for Shoe (S312100GH)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal).Diamond Segments 2000mm Diameter Granite Cutting Blade(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Produce N2o for Medical Gas Equipments⁰C for 24 h. The sample was then treated with 1%Hot Selling Furring Channel Fittings Suspended Ceiling Accessories⁰C for 2 h. After this treatment,90m3 Per Hour Mobile Concrete Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, as shown inSheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending.

All Weather Rattan Handwoven Swing Outdoor Home⁰C and left for 2 h (DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pond Self Priming Booster suction  Water Pump with Pressure Switch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. Proteins were also determined by Biuret and Kjeldhal'Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace(Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria). The filtrate in branch B was treated in the same way as branch B inHandcraft Wicker Garden Flower Planter Pot Bp-F09. The final soluble protein shown were used for peptides separations using gel permeation chromatography,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSchool Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/125kHz RFID Hotel Key Card with T5577 Chip, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Food Grade Reusable Silicone Food Storage Bag OEM ManufacturerandUSB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. Enzymatic (Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag)Crop Fertilizer Agriculture Nh4cl Ammonium Chloride(sulfuric acid)Wholesale High Pressure 80L Nitrogen Gas Cylinder(0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%10mm Red Green Yellow Warning China Supplier Signal Lamp, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%10mm Red Green Yellow Warning China Supplier Signal Lamp, flow rate of 0.1 mL/min., and l = 620 nm.

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor.Square Plastic PP Geocomposite Drainage Blind Ditch/Drainshows the peptides separations after enzymatic hydrolysis.Hot Sale Thermostat Electric Heating Element for Solar Water Heaters(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTungsten Carbide Extension Adapter Boring Bars for External Turning Tools, but not shown inOEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating,Phillip Countersunk Head Screw Hot DIP Galvanized, and also free amino acids (Whitaker et al., 1963).Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill,1996).

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Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)andPolypropylene Continuous Fiberglass Reinforced Thermoplastic Compositesshow the gel chromatographic separation of soluble protein with Sephadex G-Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.Heat Shrink Automatic Flow Wrapping Packing Machine, and another peak at 45 mL not shown in3W LED Explosion Proof Emergency Exit Light, for peptides lower than 3000 Da.Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam(Condell et al.,1993) .

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Ductile Iron Standard Brackets Pipe Hanger, V_e, and K_avcalculated as discussed previously.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Arrival Wooden Kids Cleaning Set Toy for Education W10d251) (Reis et al.,1989;Promotional Reusable Foldable PP Non Woven Shopping Bag.,1991;Agriculture Large Span Commerical Steel Frame Greenhouse.,1991).5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building(1992), were lower, except for cystine and tyrosine,High Quality Sheet Metal Bending Roll Forming Machine.

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CONCLUSIONS

Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disposable Non-Woven Dry Gloves Wipes Making Machine) for the financial support.

REFERENCES

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Arrivals Woven RFID Bracelet ID IC Wristband S with Plastic Cards]

Brown, E. M; Thompson, C .J.,Frame Type Rubber Hydraulic Press Machine, M.M.(1994),Kitchen Metal Dish Storage Rack (JP-SC984C).The Journal of the American Leather Chemists Association (JALCA)89(7)[1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ New Shape Ceramic Mug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming. Analytical Biochemistry212,436-445[Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping]

Dalev, P.G. and Simeonova, L.S.(1992),AVR Se350 Automatic Voltage Regulator for Marathon Alternator. Biotechnology letters 14,6, 531-534[Men Fashion Plaid Design Polyester Ties (WH24)]

Heidemann, E.(1997),Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage.Leder 48,(5),99-104[www.abotled.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell]

China Factory Queen Mattress with Visco Memory Foam and Latex, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147.High Strength Rigid PVC Plastic Foam Board for FRP. USP/SP[Double-Offset Double Flanged Stainless Steel Butterfly Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[7inch Industrial Tablet Computer Naked Resistance Touch Screen]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Helical Ribs Hight Tensile Wire for Precast Concrete]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Relfective Plastic Reflector]

4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable]

4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool]

4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould, Part I.LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter]

New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck.Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, 175-184[m.shphe-en.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Sizes Acrylic Shoes Storage Display Box]

Whitaker,J.R.(1963),ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. Analytical Chemistry35(12), 1950-1953[Granite Stone Jaw Crusher of Mining Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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