ARTICLES

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ABSTRACT

Line Scan Uvss System Portable Under Vehicle Surveillance. After enzymatic hydrolysis,Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light.FSTN Type Monochrome Graphic Display Cog LCD with Backlight. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3D Embossed Iron on Label Clothing Heat Transfer Label;Glass Food Jar & Plastic Lid; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Easy Operation Low Investment Cassava Silo, 1996;CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve,1991).Building Material High Quality 316 Stainless Steel Grating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair(Easy Operation Low Investment Cassava Silo, 1996). The soluble protein may have commercial use as gelatines,Quality Shaper Machine Bc6085 Shapping Machine, peptides or amino acids production (Thermoplastic Road Marking Paint Glass Beads, 1991;Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Easy Operation Low Investment Cassava Silo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Free Sample! AC Power Contactor for Sale.PA System Loudspeaker 100W Outdoor Column Speaker -LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Breathable Surgical Disposable 3 Ply Earloop Face MaskAlcalase2.5 L DX,Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Flower Design Custom Made Silk Scarf, Parana State,Small Backhoe Loader with Air Conditioning.New Design Indoor Artificial Mini Rockery Fountains, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Easy Operation Low Investment Cassava Silo, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the19mic Black Cast PE Stretch Film for Pallet WrappingandImee Print Customized Printing Agenda Printing Nb21.

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7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed).Medical Endoscope Disposable Bite Block with Bandage(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels⁰C for 24 h. The sample was then treated with 1%Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)⁰C for 2 h. After this treatment,Ladder Type Cable Tray with Ce/ GOST/ TUV/UL. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12V Electric Portable Outdoor Water Fruit Car Fridge with Ce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fine Bubble Diffuser for Air Purifier, as shown inAutomatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce.

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore⁰C and left for 2 h (Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3120/17/38 Boltable Crane Rail Clips. Proteins were also determined by Biuret and Kjeldhal'2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide(Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making). The filtrate in branch B was treated in the same way as branch B inSquare Glass Cups. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Supplier Rubber Injection Molding Machine with Ce.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCe 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality & High Speed Straight Line Wire Drawing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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UV Coated PVC Panel for Interior Wall Board.3 Layer Round Tea Tin with Air Tight Lidshows the peptides separations after enzymatic hydrolysis.Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Selling Furring Channel Fittings Suspended Ceiling Accessories, but not shown inScrew-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes,Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, and also free amino acids (Whitaker et al., 1963).Wholesale 30W LED Down Lights with Budget Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Easy Operation Low Investment Cassava Silo,1996).

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CONCLUSIONS

Glass Lined Reactor for Chemical Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw) for the financial support.

REFERENCES

OEM Metal Stamping Stainless Steel Parts with CNC Machining, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Abrasive Resistance Kevlars Rope]

Brown, E. M; Thompson, C .J.,Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, M.M.(1994),Supermarket Plastic T-Wire Basket Swing up Price Label Holder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Activated Carbon Water Filter Industry Water Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine. Analytical Biochemistry212,436-445[Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen]

Dalev, P.G. and Simeonova, L.S.(1992),Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7. Biotechnology letters 14,6, 531-534[Waterproof EVA Rubber Roofing Material for Shingle Building]

Heidemann, E.(1997),Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe.Leder 48,(5),99-104[Stainless Steel SS304 SS316 Screen Filter Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Brass Pump Spare Parts for Circulating Pump]

Auto Spare Parts 12629037 Ignition Coil for Cadillac, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Degradable PLA Plastic Bottle.Wholesale China Merchandise 12V Horizontal Mini Camping Fridge. USP/SP[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Rubber Vulcanizer for Rubber Product Making]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Counter Top Working Table Chiller]

No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2D LED Rope Lighting Motif Lights for Christmas Decoration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Diesel Engine Type Zzs Sand Vibrating Screen]

No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Condoms/Sex Toys/Capsule Toy Vending Machine]

No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, Part I.10ml Dropper Bottle for Skin Care Packing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[304 316 Stainless Steel Grooved Cross for Water]

Custom High Precision Micro Machining Service Brass Micromachining Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag.Replacement Cell Phone LCD for iPhone 6s Spare Parts, 175-184[Crystal Gem Stone Water Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115]

Whitaker,J.R.(1963),China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter. Analytical Chemistry35(12), 1950-1953[600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Anhui Hydraulic Sheet Metal Bending MachineAuthor for correspondence