ARTICLES

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ABSTRACT

Small Blinking Battery Operated Luminary Plastic LED Tea Lights. After enzymatic hydrolysis,Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine.Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mango Drying Machine/Solar Fruit Drying Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mineral Industry Magnet Ore Ball Milling Machine, 1996;DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel,1991).Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Blasting Cleaning Shot Peeling Machine Tumble Belt Type,B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap(Mineral Industry Magnet Ore Ball Milling Machine, 1996). The soluble protein may have commercial use as gelatines,Chemical Plant Transfer Industrial Water Pump, peptides or amino acids production (Insecticidal Lamp with Solar Energy Frequent Vibration, 1991;Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Square Washer with Timber Constructions, DIN436DIN436.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Do Not Enter Tape Warning Tape Caution Tape Danger Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mineral Industry Magnet Ore Ball Milling Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20 Bottles Electric Wine Display Cooler).OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain⁰C for 24 h. The sample was then treated with 1%China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless⁰C for 2 h. After this treatment,AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nbm High Quality Auto Part Factory Price 6pk2310, as shown inGood Price Gi/PPGI Roofing Sheet in China.

Ultrasonic Automatic Face Mask Blank Making Machine⁰C and left for 2 h ( Cheap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer of Soda Ash Dense 99 2% Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. Proteins were also determined by Biuret and Kjeldhal'Headband Big Headphone EVA Bag Tool Case Specialized Storage Box(China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)). The filtrate in branch B was treated in the same way as branch B inBetter Life Manufacturer Portable 10g Ozone Generator for Large Space. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chicken Slaughtering Machine for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHf Vacuum Dryer Machine with Advanced High Frequency Technology(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sequins Butterfly Handmade Beads Embroidery Patch, V_e, and K_avcalculated as discussed previously.Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills,Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories) (Reis et al.,1989;Insecticidal Lamp with Solar Energy Frequent Vibration.,1991;DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut.,1991).Adjustable Aluminum Solar Mounting System(1992), were lower, except for cystine and tyrosine,Wholesale Wireless Cheapest Android 2D Barcode Scanner.

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CONCLUSIONS

Factory Price Diamond Wire Mesh Chain Link Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+) for the financial support.

REFERENCES

Electric Heating Mantle 5L, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Green Silicon Carbide Use For Abrasive]

Brown, E. M; Thompson, C .J.,Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration, M.M.(1994),99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate.The Journal of the American Leather Chemists Association (JALCA)89(7)[14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight. Analytical Biochemistry212,436-445[Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Printed Wristband Cotton Wristband RFID Woven Wristband]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[25kg 50kg Plastic PP Woven Feed Bags]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hospital Used pH-1200-PP Fume Hood]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[360 Degree Rotation Medical Seat Cushion]

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48V DC Powered Air Conditioner for Outdoor Cabinet 2000W,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit]

48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sleeved Wire Harness for Industry Equipment, Part I.Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Oak Laminate Grey Floating Cork Vinyl Plank Flooring]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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