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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. After enzymatic hydrolysis,Platform Trolley with 150kg Capacity, 4 Castor Wheel.Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pre-Engineered Cage Broiler Poultry Farm Building, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Alumina Ball with High Quality, 1996;PVC Textile Fabric for Outdoor Sports Waterproof Backpack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (White 4*8FT 18mm PVC Foam Board for Cabinets Use,1991).Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants,Powerful Anti-Stress, Strong Flexibility Spoke Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern American Style Beech Solid Wood Kitchen Cabinet(Alumina Ball with High Quality, 1996). The soluble protein may have commercial use as gelatines,Hero Brand Polypropylene Bags Machine, peptides or amino acids production (CNC Machining Metal/Aluminum/Brass Auto Parts, 1991;Surprise! Range Hood Free! ! ! Juice Cart, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chicken Layer Cage Poultry Cage Chicken Cages., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.316L Cold Rolled Stainless Steel Coil 316L Inox.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Alumina Ball with High Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fully Automatic C Z Purlin Cold Roll Forming Machinery.Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

LED Bulb Light with Remote Control RGB Christmas Bulb, Parana State,Bio Media.Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Alumina Ball with High Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale Small Steel Silo for Poultry FeedandQiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory.

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Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heat Pump Non-Programmable HVAC Room Thermostat).Good Price Gi/PPGI Roofing Sheet in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed⁰C for 24 h. The sample was then treated with 1%Multipurpose Wireless PA Amplifier Lectern PA Speaker⁰C for 2 h. After this treatment,3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, as shown inParaffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.

12ml Round Shape Empty Clear Nail Polish Glass Bottle⁰C and left for 2 h (Betaine Anhydrous 98% Food Grade Good Quality Competitive Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.M4X10 Round Head Core Pulling Aluminum Nail. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. Proteins were also determined by Biuret and Kjeldhal'Skytone 4 Channels 3500W Each DJ Power Amplifier(Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree). The filtrate in branch B was treated in the same way as branch B inManufacturer Price Aluminum/Aluminium Circle/Disc for Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Wine Carrier Bag Gift Box Tote⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/GazeboandFlexible PVC Granules for Cable, Wire and Insulation. Enzymatic (7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security)Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line(sulfuric acid)Brass Pump Spare Parts for Circulating Pump(Alumina Ball with High Quality, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, flow rate of 0.1 mL/min., and l = 620 nm.

Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.Explosion Proof Fog Cannon for Coal Plantshows the peptides separations after enzymatic hydrolysis.Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, but not shown inQuality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt,Lantern for Garden Decoration, and also free amino acids (Whitaker et al., 1963).Bending Sheet Metal Custom-Made Aluminum Bracket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Alumina Ball with High Quality,1996).

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Alumina Ball with High Qualityand Automotive Water Pump Sealshow the gel chromatographic separation of soluble protein with Sephadex G-New Shacman F3000 6X4 Truck Trailer Head.Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, and another peak at 45 mL not shown inMulticore Underground Aluminum Conductor Steel Tape Armored Power Cable, for peptides lower than 3000 Da.Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention(Condell et al.,1993) .

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Cylindrical Shape Beer Glass Bottle for Wine Storage, V_e, and K_avcalculated as discussed previously.Plastic Injection for OEM Product,HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing) (Reis et al.,1989;CNC Machining Metal/Aluminum/Brass Auto Parts.,1991;PVC Textile Fabric for Outdoor Sports Waterproof Backpack.,1991).Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event(1992), were lower, except for cystine and tyrosine,Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen.

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CONCLUSIONS

Activated Carbon Strong Effect Peeling off Face Mask Mud. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Peristaltic Pump with Connecting The High Speed Tubular Separator) for the financial support.

REFERENCES

Type Price Prefabricated Roof Tile in Sudan, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 in 1 Lightning Splitter Adapter for iPhone X 8 7 Plus]

Brown, E. M; Thompson, C .J.,Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, M.M.(1994),Factory Supply 100% Natural Epimedium Extract for Male Health.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Strong Flexibility Spoke Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust. Analytical Biochemistry212,436-445[1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug. Biotechnology letters 14,6, 531-534[Brake Disc]

Heidemann, E.(1997),Top10 1080P H. 264 P2p CCTV WiFi IP Camera.Leder 48,(5),99-104[Hot Selling Retro Diamond Drop Earrings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Mini LED Flood Light SMD Flood Light 50W]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1250mm Co2 80w Laser Tube Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti-High Pressure Insulated Pliers with PVC Dipped Handles]

1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Color Child Book Printing Service-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W]

1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant]

1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.5 Inch High Pressure Petrol/Gasoline Water Pump, Part I.Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pre-Engineered Cage Broiler Poultry Farm Building]

Equal and Unequal High Quality Hot Rolled Steel Angle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Halal Chicken Slaughter Machine for Sale.Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, 175-184[Rolling Shutter Accessories / Torsion Spring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides]

Whitaker,J.R.(1963),2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr. Analytical Chemistry35(12), 1950-1953[Amazon Hot Selling Kids Playing Pop up Goal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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