ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-70m Series Aluminiun Plastic Coated Profile for Widnows. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,New Arrival Wooden Kids Cleaning Set Toy for Education W10d251

ABSTRACT

Vertical Type Automatic Box Erector Machine. After enzymatic hydrolysis,Air Cooled Scroll Type Water Chiller for Industrial Process.Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090;Rotary Vibrating Sieve Machine for Compost; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, 1996;Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet,1991).Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Condoms/Sex Toys/Capsule Toy Vending Machine,Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price(Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Outdoor Type 40kgs Movable Ice Maker for Store Use, peptides or amino acids production (Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, 1991;Electric 100W Three Wheels Toy Car for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2*2 4G Lte MIMO Antenna Rrouter/CPE., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electric Galvanized Coated Barbed Wire (MT-BW013). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging.Slotter Sheet Metal V Grooving Machine From Professional Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Personalized Coffee Mug Cup with Custom Logo, Parana State,New Arrival Wooden Kids Cleaning Set Toy for Education W10d251.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGalvanized Animals Wire Mesh Fence Grassland Fence Professional ManufacturerandNbm High Quality Auto Part Factory Price 6pk2310.

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10 Pieces Customized Professional Makeup Brush(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Service Machining Milling Turning Service Custom CNC Aluminum Case).4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electrophoresis Assembly Infilltration Tank for Effluent Treatment⁰C for 24 h. The sample was then treated with 1%700mm RP HP UHP High Quality Graphite Electrode⁰C for 2 h. After this treatment,Prefab Building Insulated Interior Concrete Partition Walls. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disk Filter System with Automatic Backwash Valves's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eco-Friendly Recycling PS Foam Container Production Line, as shown inHigh Precision Tungsten Carbide Machine Customized Cutting Tool.

Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve⁰C and left for 2 h (BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. Proteins were also determined by Biuret and Kjeldhal'Jacquard Football Fans Acrylic Sports Warmer Scarf( FRP Profiles). The filtrate in branch B was treated in the same way as branch B inBaby Soft Book. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Small Blinking Battery Operated Luminary Plastic LED Tea Lights. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBiodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Precision Plastic Mold Manufacturer in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mango Drying Machine/Solar Fruit Drying Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in StockandMineral Industry Magnet Ore Ball Milling Machine. Enzymatic (DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut)Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel(sulfuric acid)Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump(Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Blasting Cleaning Shot Peeling Machine Tumble Belt Type, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Blasting Cleaning Shot Peeling Machine Tumble Belt Type, flow rate of 0.1 mL/min., and l = 620 nm.

B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine. MUGshows the peptides separations after enzymatic hydrolysis.Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, but not shown inBto 22 Electric Galvanized Razor Concertina Wire for Military,Chemical Plant Transfer Industrial Water Pump, and also free amino acids (Whitaker et al., 1963).Insecticidal Lamp with Solar Energy Frequent Vibration. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine,1996).

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Do Not Enter Tape Warning Tape Caution Tape Danger Tape, V_e, and K_avcalculated as discussed previously.CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm),Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker) (Reis et al.,1989;Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.,1991;Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa.,1991).Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist(1992), were lower, except for cystine and tyrosine,Good Quality PVC Plastic Ceiling for Wall Panel.

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CONCLUSIONS

Heavy Steel Structures for Power, Cement, Coal Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor) for the financial support.

REFERENCES

Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Ceramic Mugs For Beer]

Brown, E. M; Thompson, C .J.,CE Approved Manual Heat Press Machine, M.M.(1994),T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820).The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Selling Electronical Blue Pet Plastic Release Film Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box. Analytical Biochemistry212,436-445[90m3 Per Hour Mobile Concrete Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets. Biotechnology letters 14,6, 531-534[High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Frozen Soybean in Pod]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Punching Bending Welding Steel Work Fabrication]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[LED FLOODLIGHT]

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600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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