ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Adding Water Rice Pulping Machine/Soybean Grinding Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)

ABSTRACT

Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing. After enzymatic hydrolysis,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire.Hanging Necklace Fan USB Rechargeable Battery Operated Fan. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Mushroom Rround Head Solid Rivet (Round head);Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Warm Women Suede Leisure Snow Boots for Winter (FF-1), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality Xanthan Gum Application of Food with Best Price, 1996;Wet Wire Drawing Machine for High Carbon Mattress Spring Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome,1991).Effluent Treatment Ferrous Sulphate Heptahydrate 98%. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rechargeable 9V Li Ion Battery with Micro USB Charging Port,Transport System China Manufacturer for Table Belt Conveyor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal(Good Quality Xanthan Gum Application of Food with Best Price, 1996). The soluble protein may have commercial use as gelatines,Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), peptides or amino acids production (High Quality Mini LED Flood Light SMD Flood Light 50W, 1991;Ferrite Core for Power Transformer (Ee16e), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Manufacturer Wallpaper House Decoration Wall Paper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flexible Magnetic Rubber Sheet Soft Magnetic Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality Xanthan Gum Application of Food with Best Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass.Electric Wine Bottle Opener K020-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, Parana State,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B).Diesel Engine Type Zzs Sand Vibrating Screen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Quality Xanthan Gum Application of Food with Best Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theVertical Type Caseformer Machine WJ-LKX-15and360 Rotating Automatic Touchless Car Wash Equipment with Drying System.

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Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (X-Humate 95% Min Super Sodium Humate (Nut Moradant)).Bluetooth Smart Watch with Heart Rate Monitor for Men 72087(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Trendy High Quality 925 Stering Silver Tennis Glod Charm⁰C for 24 h. The sample was then treated with 1%Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites⁰C for 2 h. After this treatment,Stand 2.5t Electric Pallet Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, as shown inPlastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump.

Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label⁰C and left for 2 h (PP Foam Stationary Sheet Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Men Fashion Plaid Design Polyester Ties (WH24). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,70m Series Aluminiun Plastic Coated Profile for Widnows. Proteins were also determined by Biuret and Kjeldhal'New Arrival Wooden Kids Cleaning Set Toy for Education W10d251(Downspout Pipe Roll Forming Machine). The filtrate in branch B was treated in the same way as branch B inPl Pneumatic Quick Connect Male Elbow Tube Fittings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vertical Type Automatic Box Erector Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Air Cooled Scroll Type Water Chiller for Industrial Process. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rotary Vibrating Sieve Machine for Compost⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping BagandChengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Enzymatic (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet)Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube(sulfuric acid)Condoms/Sex Toys/Capsule Toy Vending Machine(Good Quality Xanthan Gum Application of Food with Best Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), flow rate of 0.1 mL/min., and l = 620 nm.

Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price.Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plusshows the peptides separations after enzymatic hydrolysis.Outdoor Type 40kgs Movable Ice Maker for Store Use(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew, but not shown inWholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent,Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, and also free amino acids (Whitaker et al., 1963).Electric 100W Three Wheels Toy Car for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quality Xanthan Gum Application of Food with Best Price,1996).

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CONCLUSIONS

China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Spine Shape Landscaping Artificial Turf Grass) for the financial support.

REFERENCES

Cute Clear Fanny Pack Stadium Security PVC Waist Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[npt type cable gland]

Brown, E. M; Thompson, C .J.,Personalized Coffee Mug Cup with Custom Logo, M.M.(1994),100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Toast Stamp Loaf Bread Tin Pan (ZMA)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10 Pieces Customized Professional Makeup Brush. Analytical Biochemistry212,436-445[Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen]

Dalev, P.G. and Simeonova, L.S.(1992),4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. Biotechnology letters 14,6, 531-534[Baby Feeding Chair Plastic Chair for Feeding Hebei China]

Heidemann, E.(1997),Electrophoresis Assembly Infilltration Tank for Effluent Treatment.Leder 48,(5),99-104[Clip Fan]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust]

Chemical Product Sodium Gluconate Used for Concrete Admixture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),700mm RP HP UHP High Quality Graphite Electrode.Prefab Building Insulated Interior Concrete Partition Walls. USP/SP[Mineral Industry Magnet Ore Ball Milling Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CS1363 a Standard UK 3 Pin Power Plug]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Disk Filter System with Automatic Backwash Valves: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Elastic Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Qg Steel Pipe Shot Blast Cleaning Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts]

Eco-Friendly Recycling PS Foam Container Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Bulb Light with Remote Control RGB Christmas Bulb]

Eco-Friendly Recycling PS Foam Container Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Lgd4033]

Eco-Friendly Recycling PS Foam Container Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, Part I.High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bluetooth Smart Watch with Heart Rate Monitor for Men 72087]

Jacquard Football Fans Acrylic Sports Warmer Scarf, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.Small Blinking Battery Operated Luminary Plastic LED Tea Lights, 175-184[Wholesale Custom Logo Leather Notebook]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Marine High Visibility Solas Reflective Tape]

Whitaker,J.R.(1963),Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine. Analytical Chemistry35(12), 1950-1953[Maleic Anhydride CAS 108-31-6]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Feet Protection Industrial Cow Split Leather Working Safety ShoesAuthor for correspondence