ARTICLES

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ABSTRACT

Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). After enzymatic hydrolysis,1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction.Water Treatment Anthracite. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heat Pump Non-Programmable HVAC Room Thermostat, 1996;Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable,1991).Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator,Injector Pump Calibration Test Bench,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASME/ASTM Butt-Welding Stainless Steel Plate Flange(Heat Pump Non-Programmable HVAC Room Thermostat, 1996). The soluble protein may have commercial use as gelatines,500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, peptides or amino acids production (Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, 1991;Polyester / Polypropylene Woven Geotextile for Road Construction, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Granite Stone Jaw Crusher of Mining Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heat Pump Non-Programmable HVAC Room Thermostat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine.Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale All Kinds Cheap Plush Animal Baby Slippers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Airtight Candy Bin with Scoop(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10 Pieces Customized Professional Makeup Brush).Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell⁰C for 24 h. The sample was then treated with 1%3 Layer Round Tea Tin with Air Tight Lid⁰C for 2 h. After this treatment,China PA System Power Amplifier with Enough Rated Power. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Export Grade Citric Acid Monohydrate as Detergent's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, as shown inHigh Quality Crusher for Used Plastic Recycling Line.

Familu Use Mini Small Pig Feed Pellet Mill⁰C and left for 2 h (PVC Textile Fabric for Outdoor Sports Waterproof Backpack). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. Proteins were also determined by Biuret and Kjeldhal'Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed(Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer). The filtrate in branch B was treated in the same way as branch B inSMP-04 AC Mini Submersible Pump for Circulation. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hairdressing Equipment Takara Belmont Barber Chair High Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuy Single Item Moving Bee Eye Light Lowly Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rectangular Box Facial Tissue Paper Fk-110, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Galvanized Bar Grating.Nbm High Quality Auto Part Factory Price 6pk2310shows the peptides separations after enzymatic hydrolysis.High Quality Custom Self Adhesive Leather Patch for Jackets(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGarlic Red Mesh Bag, but not shown inOEM Service Machining Milling Turning Service Custom CNC Aluminum Case,Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale, and also free amino acids (Whitaker et al., 1963).380mm Working Area Concrete Diamond Floor Edge Grinder. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Heat Pump Non-Programmable HVAC Room Thermostat,1996).

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CONCLUSIONS

U. S. Type Drop Forged Wire Rope Clip. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine) for the financial support.

REFERENCES

Humizone Seaweed Customized Organic Liquid Fertilizer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Food Grade Stainless Steel Multi-Cartridge Filter Housing]

Brown, E. M; Thompson, C .J.,Zipper Fabric Travel Passport Wallet Organiser, M.M.(1994),Hot Sale Instax Mini Camera Korea Style Photo Album.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Automatic Packing Machine (GK-650 BA)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Paint Sprayer Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Low Profile Adjustable Bracket. Analytical Biochemistry212,436-445[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

Dalev, P.G. and Simeonova, L.S.(1992),Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. Biotechnology letters 14,6, 531-534[316L Cold Rolled Stainless Steel Coil 316L Inox]

Heidemann, E.(1997),High Quality Acrylic Beer Display Racks with LED.Leder 48,(5),99-104[Children Bicycle Without Pedal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4 Seater Pessenger Golf Cart Dg-C4 with Ce Certificate (China)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Selling Electronical Blue Pet Plastic Release Film Price]

Plastic Injection Molding for Auto Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Round Type Twin Screw Extruder Barrel Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[90m3 Per Hour Mobile Concrete Pump]

Plastic Injection Molding for Auto Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing]

Plastic Injection Molding for Auto Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASA-UPVC Anti-Corrosive Composite Roof Tile, Part I.Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Frozen Soybean in Pod]

Sexy Mesh Transparent Female Running Tank Top with Hood Custom, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Color Cardstock Color Paper Manufacturer.Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, 175-184[Wood Look UV Print Crystal Glass Mosaic for Interior Design Decoration (AMKGMT3202)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer]

Whitaker,J.R.(1963),Home and Office PVC Vinyl Flooring Conductive Plank Flooring. Analytical Chemistry35(12), 1950-1953[Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Punching Bending Welding Steel Work FabricationAuthor for correspondence