ARTICLES

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ABSTRACT

Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part. After enzymatic hydrolysis,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults.Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01);Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plain Makeup Bag Canvas Cosmetic Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, 1996;1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple,1991).Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Wholesale Qualited Polyester Padding Men Jacket,5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer(14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, 1996). The soluble protein may have commercial use as gelatines,Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration, peptides or amino acids production (Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, 1991;Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;River Water Cleaning Sludge Sediment Machine Lamella Clarifier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polyester Coated Stainless Steel Ladder Type Metal Ties.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder.Copper Laminated Steel Strip T2/C11000-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Halal Empty Gelatin Capsule for Medicine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nbm High Quality Auto Part Factory Price 6pk2310).Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet⁰C for 24 h. The sample was then treated with 1%1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price⁰C for 2 h. After this treatment,Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, as shown inGarlic Red Mesh Bag.

Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength⁰C and left for 2 h (OEM Service Machining Milling Turning Service Custom CNC Aluminum Case). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Food Box Container Covers by Injection Mould Mold Tool. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. Proteins were also determined by Biuret and Kjeldhal'Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(High Precision Tungsten Carbide Machine Customized Cutting Tool). The filtrate in branch B was treated in the same way as branch B inVertical Lines Canvas Fashion Handbag Wholesale Guangzhou Lady Handbags Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Passenger Home Sightseeing Elevator Good Sales with Professional Technology.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vacuum Stainless Steel Casting Products. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChicken Farming Materials Poultry Battery Cages Layer Battery Cage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.I-Tech 12000HD 2u Space Digital Professional Power Amplifier⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Silicone Reusable Food Storage Bag China Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing FilmandPolyurethane Gasket Foam Seal Dispensing Machine. Enzymatic (Imee Print Customized Printing Agenda Printing Nb21)Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml(sulfuric acid)High Performance Multifunctional Crawler Mini Skid Steer Loader(14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, flow rate of 0.1 mL/min., and l = 620 nm.

Chunke Portable UV Water Sterilizer for Water Purifier 150t. FRP Profilesshows the peptides separations after enzymatic hydrolysis.Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, but not shown in MUG,Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, and also free amino acids (Whitaker et al., 1963).Xjm Series Rubber Extruding Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow,1996).

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Industry Pet Recycling Systems, Industry Pet Recycling Line, V_e, and K_avcalculated as discussed previously.Sublimation Polyester Sports Tshirt for Adult,Heavy Duty Marquee Advertising Pop up Tent. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar) (Reis et al.,1989;Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition.,1991;1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.,1991).Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer(1992), were lower, except for cystine and tyrosine,Fire Extinguisher UV Curing Machine Cylinder Drying Machine.

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CONCLUSIONS

Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck) for the financial support.

REFERENCES

Energy-Saving Biogas Double Stove for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Tablet Laptop Case Cover for Macbook]

Brown, E. M; Thompson, C .J.,Valve Bag Dry Mortar Bagging Machine, M.M.(1994),High Quality 500m 100lb Super Strong Braided Fishing Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. Analytical Biochemistry212,436-445[Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar]

Dalev, P.G. and Simeonova, L.S.(1992),37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light. Biotechnology letters 14,6, 531-534[Hot Selling Electronical Blue Pet Plastic Release Film Price]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[90m3 Per Hour Mobile Concrete Pump]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Frozen Soybean in Pod]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Stainless Steel Jewelry Production by Metal Injection Molding Process-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Punching Bending Welding Steel Work Fabrication]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Directly Selling Engine Standard Spark Plug, Part I.Custom Logo Silicone Wristband, Rubber Wrist Bands. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment]

Custom All Kinds of Acrylic Shoe Display Racks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide.Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, 175-184[Agricultural Machinery Forging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring]

Whitaker,J.R.(1963),Medical Supply U40 Disposable Insulin Syringe. Analytical Chemistry35(12), 1950-1953[304 &314 Stainless Steel Seamless Bending Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and EfficientAuthor for correspondence