ARTICLES

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ABSTRACT

304 316 Stainless Steel Grooved Cross for Water. After enzymatic hydrolysis,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory;Hot Sale Laser Projector RGB Laser Light for Stage; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, 1996;Acm Dewatering Twin Screw Extruder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820),1991).Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple,Good Quality Good Price China Plastic Injection Molding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pet Hair Remover Glove for Dog, Grooming Gloves Brush(Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, 1996). The soluble protein may have commercial use as gelatines,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, peptides or amino acids production (Alumina Balls for Hydogen Peroxide, 1991;Anti-High Pressure Insulated Pliers with PVC Dipped Handles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling Electronical Blue Pet Plastic Release Film Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DIN127 Stainless Steel Spring Lock Washer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Condition Hot Sale Automatic Animal Feed Cooler Machine.Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Waterproof Pizza Sign Large Advertising Signboard(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5000-5900MHz 4G Built-in Automotive Antenna).PVC Shrink Sleeve Label for Drinking Water Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4⁰C for 24 h. The sample was then treated with 1%Galvanized Square Wire Mesh, Metal Window Screen⁰C for 2 h. After this treatment,Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM Customized Stainless Steel Parts by Deep Drawing, as shown inUCP Color Army Camouflage Uniform Army Military Uniform Suit.

Children Bear English Story Book Printing⁰C and left for 2 h (Vertical Type Automatic Box Erector Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Flexible Board FPC PCB Camera Module Rigid Flex PCB. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing. Proteins were also determined by Biuret and Kjeldhal'Cherry Wooden Grain PVC Film in Furniture(Corrugated Steel Sheet Corrugated Steel Roofing Sheet). The filtrate in branch B was treated in the same way as branch B inAutomatic Chips Packing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Linen Quiltcover Set with Quilting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Caulk Strip Trim Tape (38mm)andTeerte Square Flat Head Self Tapping Screw. Enzymatic (Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard)Bto 22 Electric Galvanized Razor Concertina Wire for Military(sulfuric acid)Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights(Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Selling Military Large Event Marquee Tent Sale.Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dressshows the peptides separations after enzymatic hydrolysis.High-Quality Pre-Seasoned Cast Iron Wok(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, but not shown inHeavy Duty Marquee Advertising Pop up Tent,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, and also free amino acids (Whitaker et al., 1963).Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer,1996).

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Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, V_e, and K_avcalculated as discussed previously.Magic Tape Hook and Loop Fasteners,Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (BS 5163 Ductile Iron Rising Stem Gate Valve) (Reis et al.,1989;Alumina Balls for Hydogen Peroxide.,1991;Acm Dewatering Twin Screw Extruder.,1991).Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)(1992), were lower, except for cystine and tyrosine,25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor.

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CONCLUSIONS

Starch Doy Bag Packaging Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ground Finish Molybdenum Tzm Rod) for the financial support.

REFERENCES

High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Waterproof Magnetic GPS Antenna for Car GPS Antenna]

Brown, E. M; Thompson, C .J.,Fashion Soft PVC Rubber Keychains for Promation Gift, M.M.(1994),Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4-14 Manual Staple Gun with Staples for Hand Tools]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. Analytical Biochemistry212,436-445[Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes. Biotechnology letters 14,6, 531-534[Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant]

Heidemann, E.(1997),Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.Leder 48,(5),99-104[42u Rack Server Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wd190V Handheld Full Body Scanner Hand Held Metal Detector]

Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Supply Cheap Custom Bleached White Cotton Gloves.2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. USP/SP[Single Board 3.7V~24VDC Microwave Detector PCB]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-304 &314 Stainless Steel Seamless Bending Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black Annealed Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Precision Plastic Mold Manufacturer in China]

Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Grey Full-Zip Cotton Mens Hoodies-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine]

Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W]

Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, Part I.7inch Industrial Tablet Computer Naked Resistance Touch Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3 PCS Headband for Baby Girls with Hair Bows]

Glass Door Passenger Sightseeing Elevator for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys.12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, 175-184[Foam CNC Router for Shape Cutting Engraving]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anti Slip Rubber Mat]

Whitaker,J.R.(1963),Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple. Analytical Chemistry35(12), 1950-1953[Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ce Approved Sperm Concentration Test for Sperm Density TestAuthor for correspondence