ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Plastic Injection Molding for Auto Parts

ABSTRACT

Round Type Twin Screw Extruder Barrel Manufacturer. After enzymatic hydrolysis,ASA-UPVC Anti-Corrosive Composite Roof Tile.Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sexy Mesh Transparent Female Running Tank Top with Hood Custom;Color Cardstock Color Paper Manufacturer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Home and Office PVC Vinyl Flooring Conductive Plank Flooring, 1996;Precision Engineering Parts Bearing Support Unit for Ball Screw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection,1991).165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silicone Cake Mold Sinle Cupcake DIY,Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing(Home and Office PVC Vinyl Flooring Conductive Plank Flooring, 1996). The soluble protein may have commercial use as gelatines,Cross Stripe Sleeveless Yoga Wear, peptides or amino acids production (Construction & Decoration ERW Square Steel Tube Manufacturer, 1991;DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Steel ASTM A192 Seamless Pipe Sch120., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Excavator Radiator /Oil Radiator /Water Radiator Manufacture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Home and Office PVC Vinyl Flooring Conductive Plank Flooring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film.Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machining Checking Auto Car Checking Fixture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, Parana State,Plastic Injection Molding for Auto Parts.Original Stainless Steel Singer Brand Sewing Machine Needles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Home and Office PVC Vinyl Flooring Conductive Plank Flooring, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTeerte Square Flat Head Self Tapping ScrewandCustom Sheet Metal Stamping Bending Product Manufacturing Service.

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Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)).Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve⁰C for 24 h. The sample was then treated with 1%Steel Bar Peeling Lathe Machine⁰C for 2 h. After this treatment,Chemical Product Sodium Gluconate Used for Concrete Admixture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Adding Water Rice Pulping Machine/Soybean Grinding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), as shown in13.56 MHz Fudan FM11RF08 Chip Card.

Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing⁰C and left for 2 h (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hanging Necklace Fan USB Rechargeable Battery Operated Fan. Proteins were also determined by Biuret and Kjeldhal'Aluminum Mushroom Rround Head Solid Rivet (Round head)(Colombia Outdoor P5 Pantallas LED Display). The filtrate in branch B was treated in the same way as branch B inThree Phase 10KW/12KW/15KW/20KW/25KW. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Warm Women Suede Leisure Snow Boots for Winter (FF-1). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Quality Xanthan Gum Application of Food with Best Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Effluent Treatment Ferrous Sulphate Heptahydrate 98%, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rechargeable 9V Li Ion Battery with Micro USB Charging Port, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Food Package Aluminum Foil Tray Making MachineandTransport System China Manufacturer for Table Belt Conveyor. Enzymatic (Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal)Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)(sulfuric acid)High Quality Mini LED Flood Light SMD Flood Light 50W(Home and Office PVC Vinyl Flooring Conductive Plank Flooring, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ferrite Core for Power Transformer (Ee16e), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ferrite Core for Power Transformer (Ee16e), flow rate of 0.1 mL/min., and l = 620 nm.

Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel.5W Ultra-Thin Tiltable LED Down Lightshows the peptides separations after enzymatic hydrolysis.China Manufacturer Wallpaper House Decoration Wall Paper(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectrophoresis Assembly Infilltration Tank for Effluent Treatment, but not shown inPA System Loudspeaker 100W Outdoor Column Speaker ,Flexible Magnetic Rubber Sheet Soft Magnetic Tape, and also free amino acids (Whitaker et al., 1963).Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Home and Office PVC Vinyl Flooring Conductive Plank Flooring,1996).

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Soosan Excavator Hydraulic Hammer for Construction Demolitionand130HP 4WD Large Farm Power Engine Tractor From High Quality Factoryshow the gel chromatographic separation of soluble protein with Sephadex G-Electric Wine Bottle Opener K020.Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, and another peak at 45 mL not shown inViton O Ring Kit with Excellent with Oil Resistance, for peptides lower than 3000 Da.China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery(Condell et al.,1993) .

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China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), V_e, and K_avcalculated as discussed previously.Spine Shape Landscaping Artificial Turf Grass,08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam) (Reis et al.,1989;Construction & Decoration ERW Square Steel Tube Manufacturer.,1991;Precision Engineering Parts Bearing Support Unit for Ball Screw.,1991).3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board(1992), were lower, except for cystine and tyrosine,Diesel Engine Type Zzs Sand Vibrating Screen.

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CONCLUSIONS

Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest) for the financial support.

REFERENCES

Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Colorful Printing Shrink Wrap Bottle Labels]

Brown, E. M; Thompson, C .J.,Trendy High Quality 925 Stering Silver Tennis Glod Charm, M.M.(1994),Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10g Non-Woven Fabric Silica Gel Desiccant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stand 2.5t Electric Pallet Truck. Analytical Biochemistry212,436-445[Plastic Injection for OEM Product]

Dalev, P.G. and Simeonova, L.S.(1992),Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. Biotechnology letters 14,6, 531-534[Passenger Home Sightseeing Elevator Good Sales with Professional Technology]

Heidemann, E.(1997),Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers.Leder 48,(5),99-104[Popular Cola Bottle Washing Plant for Crushing Recycling Plastic Pet PE PP Bottles with Label Separator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V]

Baby Feeding Chair Plastic Chair for Feeding Hebei China, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label.Men Fashion Plaid Design Polyester Ties (WH24). USP/SP[Polyester / Polypropylene Woven Geotextile for Road Construction]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cummins L10 Engine Piston Cooling Nozzle 3070218]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-70m Series Aluminiun Plastic Coated Profile for Widnows: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Xanthan Gum for Personal Care and Cosmetic Applications]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Children Dresses]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)]

New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vertical Type Automatic Box Erector Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Black Epoxy]

New Arrival Wooden Kids Cleaning Set Toy for Education W10d251,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut]

New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Air Cooled Scroll Type Water Chiller for Industrial Process, Part I.Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Produce N2o for Medical Gas Equipments]

Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rotary Vibrating Sieve Machine for Compost.I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, 175-184[Dewatering Screw Press Machine for Paint Sludge Treatment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bobcat Skid Steer Loader Bucket Teeth 6737325lp]

Whitaker,J.R.(1963),Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Analytical Chemistry35(12), 1950-1953[2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare PartsAuthor for correspondence