ARTICLES

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ABSTRACT

Ductile Iron Flanged Swing Check Valve. After enzymatic hydrolysis,Ozonated Water for Vegetables and Fruits Washing Ozone Generator.Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Day and Night Zebra Blind Fabric Z1602;40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Impact Sound Isolation Wall Clip, 1996;Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (German Technology Automatic Stacking System in Red Brick Automatic Production Line,1991).Top Quality Galvanized Iron Wire as Binding Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,13.56 MHz Fudan FM11RF08 Chip Card,Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(Impact Sound Isolation Wall Clip, 1996). The soluble protein may have commercial use as gelatines,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, peptides or amino acids production (100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap, 1991;Square Premium Glass Perfume Bottle 100ml, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Impact Sound Isolation Wall Clip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing.High Quality Inflatable PVC Flocking Health Bed-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cast Iron Butterfly Valve with Handles).Hexagonal Netting/ Gabion Box/ Gabion Basket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Different Thickness High Quality Aluminum Foil for Glass Bottle Lid⁰C for 24 h. The sample was then treated with 1%Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla⁰C for 2 h. After this treatment,Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, as shown inProducts Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots.

Stainless Steel Ozone Disinfection Machine Ozone Generator⁰C and left for 2 h (U. S. Type Drop Forged Wire Rope Clip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PVC Profile with Net Making Machine Used for Window Sealing Profile. Proteins were also determined by Biuret and Kjeldhal'Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch(Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures). The filtrate in branch B was treated in the same way as branch B inN52 Customized Micro Cylinder Permanent Neodymium Rod Magnets with Package. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bobby Flay Websites Restaurant Martha Stewart Designer Tray.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAirport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Travelling Weekender Mens Canvas Duffle Gym Sports Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)andChina Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). Enzymatic (Best Selling Household Living Room Mat Chenille Carpets)130HP 4WD Large Farm Power Engine Tractor From High Quality Factory(sulfuric acid)Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment(Impact Sound Isolation Wall Clip, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Large Size Outdoor Store Front Signs Advertising Billboard, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Large Size Outdoor Store Front Signs Advertising Billboard, flow rate of 0.1 mL/min., and l = 620 nm.

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015grshows the peptides separations after enzymatic hydrolysis.1325 CNC Engraving Router(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale All Kinds Cheap Plush Animal Baby Slippers, but not shown in12ml Round Shape Empty Clear Nail Polish Glass Bottle,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, and also free amino acids (Whitaker et al., 1963).T6s 86 PCD Core Bit for Geotechnical Drilling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Impact Sound Isolation Wall Clip,1996).

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Round Shape All Color Pets Feeding Dog Bowl of China Supplies, V_e, and K_avcalculated as discussed previously.Wvrb Series Planetary Gearbox Used for Robot,NPK 15 15 15 Compound Fertilizer at a Hot Selling. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Large Batches Equipoise Light Yellow Liquids) (Reis et al.,1989;100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap.,1991;Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving.,1991).Mini Blown Film Extrusion(1992), were lower, except for cystine and tyrosine,Pearls Flake 99% Factory Naoh Caustic Soda.

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CONCLUSIONS

Auto Spare Parts 12629037 Ignition Coil for Cadillac. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer) for the financial support.

REFERENCES

High Quality PVC Resin with Low Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[North Benz Ng80 6X6 Tractor Truck 450 HP Engine]

Brown, E. M; Thompson, C .J.,Small Backhoe Loader with Air Conditioning, M.M.(1994),Line Scan Uvss System Portable Under Vehicle Surveillance.The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Manufacture Commercial Use Spice Grinder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. Analytical Biochemistry212,436-445[Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts]

Dalev, P.G. and Simeonova, L.S.(1992),FSTN Type Monochrome Graphic Display Cog LCD with Backlight. Biotechnology letters 14,6, 531-534[Korean Integrated CTO Activated Carbon Filter for Water Treatment]

Heidemann, E.(1997),3D Embossed Iron on Label Clothing Heat Transfer Label.Leder 48,(5),99-104[Delixi Cw1 Low Voltage 220V 6300A 3p Drawer Frame Universal Air Circuit Breaker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using]

Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Glass Food Jar & Plastic Lid.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. USP/SP[0.5 Gallons Cool Mist Ultrasonic Humidifier]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Easy Operation Low Investment Cassava Silo: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Promotional Customized PP Hand Fan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Corrugated Metal Steel Color Coated / Prepainted for Iron Roofing Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Plastic Part Hardware Plastic Injection Mold]

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine]

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Chemical Plant Transfer Industrial Water Pump]

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Building Material High Quality 316 Stainless Steel Grating, Part I.Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital]

Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair.Quality Shaper Machine Bc6085 Shapping Machine, 175-184[Hex 22 Tapered Rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector]

Whitaker,J.R.(1963),Thermoplastic Road Marking Paint Glass Beads. Analytical Chemistry35(12), 1950-1953[60m3/H Lamella Clarifier for River Water Treatment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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