ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Swimming Pool Water Disinfect Ozone Generator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,20kv 50kVA High Voltage Dry Type Power Supply Transformer

ABSTRACT

Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. After enzymatic hydrolysis,High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter.4 Styles Blue Glass Crystal Butt Beads Spiral Plug. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve;Industrial Activated Carbon Water Filter Industry Water Treatment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2016 New Generation Sausage Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), 1996;Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03),1991).China Supplier Building Construction Steel Frame (ZY431). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing,Lab Equipment portable Fume Hood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt(Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), 1996). The soluble protein may have commercial use as gelatines,10ton Telescopic Hydraulic Truck Crane, peptides or amino acids production (Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV, 1991;for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cracker Multi Rows Trayless Packaging Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2 Quick Change Flexible Roloc Disc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack.High Quality Aluminium Windows Thermal Break China Supplier-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs, Parana State,20kv 50kVA High Voltage Dry Type Power Supply Transformer.Powder Coated H Column Beam Steel Structure Building for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas CylinderandFashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap.

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Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Price Printing Doy Pack Bag).Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut⁰C for 24 h. The sample was then treated with 1%Nylon Cable Glands with CE⁰C for 2 h. After this treatment,X Shaped Extruded Aluminum Window Screen Frames. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4, as shown inXjm Series Rubber Extruding Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQ235 Jr Hollow Structual Section Steel Tubes with Good Quanlity(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Crusher for Used Plastic Recycling Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury SupplierandSS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving. Enzymatic (Wear Resitant Rope End Cap Plastic Flange Cover)Comfortable Two Piece Women Fitness Clothing for Gym Wear(sulfuric acid)Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner(Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Flat Surface Mounted 300mm 24W LED Panel Light for Interior, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Flat Surface Mounted 300mm 24W LED Panel Light for Interior, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Home Kitchen Water Purification Ozone Generator 1000mg. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace) for the financial support.

REFERENCES

PP Foam Stationary Sheet Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stand Book Wine Box Lamp Artwork Wire Shelves]

Brown, E. M; Thompson, C .J.,Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640), M.M.(1994),Economical Residential Home Automatic Aluminum Rolling Shutter Door.The Journal of the American Leather Chemists Association (JALCA)89(7)[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Clear PVC Sheet with Coating for Card. Analytical Biochemistry212,436-445[Original Stainless Steel Singer Brand Sewing Machine Needles]

Dalev, P.G. and Simeonova, L.S.(1992),Classic Black and White Plaid Sling Bag for Women. Biotechnology letters 14,6, 531-534[19mic Black Cast PE Stretch Film for Pallet Wrapping]

Heidemann, E.(1997),24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable.Leder 48,(5),99-104[Brew Works Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Imee Print Customized Printing Agenda Printing Nb21]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Newly Fine Aluminium Copper Powder Shredder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine]

100% Acrylic Knitted Sports Woven Scarf, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Prefab Steel Structure Shed for Storage Warehouse-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making]

100% Acrylic Knitted Sports Woven Scarf,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Wire Diameter 18AWG]

100% Acrylic Knitted Sports Woven Scarf, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, Part I.Gen 2+ or 3 Day/Night Vision Scope. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3 Layer Round Tea Tin with Air Tight Lid]

Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA.1650mm*80mm Stable Power Laser Tube, 175-184[2.5 Inch Tft 489 Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling Furring Channel Fittings Suspended Ceiling Accessories]

Whitaker,J.R.(1963),FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit. Analytical Chemistry35(12), 1950-1953[Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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