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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Adjustable Aluminum Solar Mounting System, 1996;Wholesale Wireless Cheapest Android 2D Barcode Scanner., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Price Diamond Wire Mesh Chain Link Fence,1991).Electric Heating Mantle 5L. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration,99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight(Adjustable Aluminum Solar Mounting System, 1996). The soluble protein may have commercial use as gelatines,Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube, peptides or amino acids production (Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, 1991;FRP GRP Fiber Reinforced Plastic Fiberglass Gratings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-48V DC Powered Air Conditioner for Outdoor Cabinet 2000W. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Adjustable Aluminum Solar Mounting System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides.Sleeved Wire Harness for Industry Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bio Media).New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase⁰C for 24 h. The sample was then treated with 1%High Quality Army Captain Navy Blue Color Hat for Army Tender⁰C for 2 h. After this treatment,Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Efficiency Mini-Pleat HEPA Filter (H13/H14)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Vertica Parking Garage System, as shown in28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Three Side Sealing Facial Mask Bag Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Manufacture Commercial Use Spice Grinder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1.5/2 power 50Hz ZN SERIES Vibrator motor.Auto Car Accessories Seat for Childshows the peptides separations after enzymatic hydrolysis.Good Quality NBR70 O-Ring with Reasonable Prices(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service, but not shown inRigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding,Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, and also free amino acids (Whitaker et al., 1963).OPP Printed Block Bottom Bags. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Adjustable Aluminum Solar Mounting System,1996).

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CONCLUSIONS

Four Color Long Sleeve Yoga Wear. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1.75mm 3mm ABS PLA PC 3D Printer Filament Machine) for the financial support.

REFERENCES

Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Zoomlion 55 Ton Mobile Crane Qy55 Truck Crane for Sale]

Brown, E. M; Thompson, C .J.,China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, M.M.(1994),Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck. Analytical Biochemistry212,436-445[High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding]

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Heidemann, E.(1997),Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient.Leder 48,(5),99-104[TPR Shoe Material Thermoplastic Rubber/Wood Pellet Production Line/Plastic Wood Composite Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Price 6000 Series Industrial Extruded Aluminum Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Glass Salt & Pepper Mill/Grinder Glass Spice Jar]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10% off Discount Aspartame Price Aspartame Sweetener CAS 22839-47-0]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[All Size Colored Tapping Hex Head Self Drill Screw Wood Screw]

WPC Decking Profile Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Frequency 3000W 24V 230V Inverter with Battery Charger]

WPC Decking Profile Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ss400 Galvanized Steel C Channel Steel Price]

WPC Decking Profile Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, Part I.Party Mask LED Fiber Dance Princess Feather Mask. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)]

80GSM C2s Coated Glossy/Matt Art Paper for Printing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter.Reusable Silicone Straight Straws, 175-184[M8 Waterproof Aviation Plug Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera]

Whitaker,J.R.(1963),Easy Foldable Kids Walking Chair Toys Baby Walker. Analytical Chemistry35(12), 1950-1953[Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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