ARTICLES

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ABSTRACT

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. After enzymatic hydrolysis,OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic.PP Woven Bag, Rice, Beans, Plastic Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Professional Mold Factory Plastic Injection Mould Maker;Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Carbon Steel Wire Hose Hydraulic Pipe, 1996;24k Gold Whitening Anti-Spot Mask., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Clay Tiles Making Machine with Latest Technology,1991).DIN Standard Gratings/Gavlanized Steel Grating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,50mm Magnetic River Water Sewage Flow Meter for Acid,PE Biodegradable Sheet Product Line/Single Screw Extruder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,27L/Min Water Exchanger Heat Pump Mold Temperature Machine(Industrial Carbon Steel Wire Hose Hydraulic Pipe, 1996). The soluble protein may have commercial use as gelatines,Felt Belt for Textile Compactor, peptides or amino acids production (Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, 1991;Metal Soft Enamel Souvenir Metal Medal Sport Medallions, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20L Water Tank Lid Mold 3D Printing Samples Available., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale High Quality Metal Car Mount Holder Phone Holder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Carbon Steel Wire Hose Hydraulic Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns.Pet Laminate Aluminium Foil/Pet+Alu+PE-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

New Design Multi-Color Silicone Coin Purse for LadyAlcalase2.5 L DX,Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Dark Green Plastic PVC Film for Christmas Tree Leaves(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, Parana State,New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Industrial Carbon Steel Wire Hose Hydraulic Pipe, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSteel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry FurnaceandTop Quality Waterproof IP66 Aluminum Die Cast Junction Box.

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1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heavy Duty Wood Door Calibrating Wide Belt Sander).Water Treatment Anthracite(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heat Treated Welding Protection Glassfiber Cloth Ht800⁰C for 24 h. The sample was then treated with 1%Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring⁰C for 2 h. After this treatment,Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heat Pump Non-Programmable HVAC Room Thermostat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, as shown inHigh Safety Double Wire Security Metal Welded Wire Mesh Fence.

Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable⁰C and left for 2 h (Humic Acid Bio-Fertilizer Granule Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. Proteins were also determined by Biuret and Kjeldhal'Injector Pump Calibration Test Bench(Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label). The filtrate in branch B was treated in the same way as branch B inCute Sweatband. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ASME/ASTM Butt-Welding Stainless Steel Plate Flange.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Granite Stone Jaw Crusher of Mining Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)andThe Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. Enzymatic (Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag)Wholesale All Kinds Cheap Plush Animal Baby Slippers(sulfuric acid)Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car(Industrial Carbon Steel Wire Hose Hydraulic Pipe, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, flow rate of 0.1 mL/min., and l = 620 nm.

Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.Nylon Balance Bike with Water Transfer Printing Malaysiashows the peptides separations after enzymatic hydrolysis.New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV, but not shown inAngle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam,Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale, and also free amino acids (Whitaker et al., 1963).Taiding Custom Tank Sheet Metal Fabrication. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Carbon Steel Wire Hose Hydraulic Pipe,1996).

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3 Layer Round Tea Tin with Air Tight Lid, V_e, and K_avcalculated as discussed previously.China PA System Power Amplifier with Enough Rated Power,Export Grade Citric Acid Monohydrate as Detergent. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming) (Reis et al.,1989;Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt.,1991;24k Gold Whitening Anti-Spot Mask.,1991).Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace(1992), were lower, except for cystine and tyrosine,Familu Use Mini Small Pig Feed Pellet Mill.

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CONCLUSIONS

3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen) for the financial support.

REFERENCES

Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Glass Clamps]

Brown, E. M; Thompson, C .J.,Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, M.M.(1994),Hairdressing Equipment Takara Belmont Barber Chair High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Interior Metal 3D Bee Garden Ornament Decoration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000). Analytical Biochemistry212,436-445[Factor Direct Adult Sex Toy Doll for Male Sex]

Dalev, P.G. and Simeonova, L.S.(1992),Buy Single Item Moving Bee Eye Light Lowly Price. Biotechnology letters 14,6, 531-534[UTP CAT6 48 Ports Vertical Termination Patch Panel]

Heidemann, E.(1997),High Quality Customized Aluminum Foil Shisha Sheet.Leder 48,(5),99-104[Electronic Refrigeration Parts Temperature Controller Stc-8080A]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminium Unitized Curtain Wall for Office Building]

Customized Anti-Rust Torsion Spring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank.Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment. USP/SP[Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Iron Sheet Metal CNC Press Brake]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rectangular Box Facial Tissue Paper Fk-110: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Milk Cream Disc Centrifugal Separator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black Phosphate Drywall Screw High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts]

SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments]

SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, Part I.CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[304 Stainless Steel Standard V-Band Clamps for Turbo]

Q32 Series Tumble Belt/Crawler Shot Blasting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Bar Grating.High Quality Custom Self Adhesive Leather Patch for Jackets, 175-184[New Coming Wet Glue Cheese Dip Labeling Machines]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

Whitaker,J.R.(1963),Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale. Analytical Chemistry35(12), 1950-1953[High-Efficient Htk Walnut Shell Filter for Water Purification]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet TilesAuthor for correspondence