ARTICLES

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ABSTRACT

Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. After enzymatic hydrolysis,High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine.Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chinese Jbcz Handy Operation Threading Machine and Coupler;Promotional Reusable Foldable PP Non Woven Shopping Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price Printing Doy Pack Bag, 1996;Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes,1991).Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17,Engine Parts Rubber High Quality Timing Belt 111ru25,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Interior Metal 3D Bee Garden Ornament Decoration(Factory Price Printing Doy Pack Bag, 1996). The soluble protein may have commercial use as gelatines,Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, peptides or amino acids production (Disposable Non-Woven Dry Gloves Wipes Making Machine, 1991;ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bottom Pushing for Fire Extinguisher Hot Spinning Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fish Scales Texture Fashion Leather for Shoe (S312100GH). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price Printing Doy Pack Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Diamond Segments 2000mm Diameter Granite Cutting Blade.Produce N2o for Medical Gas Equipments-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Furring Channel Fittings Suspended Ceiling Accessories(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve).for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree⁰C for 24 h. The sample was then treated with 1%School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)⁰C for 2 h. After this treatment,Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, as shown inChina Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery.

125kHz RFID Hotel Key Card with T5577 Chip⁰C and left for 2 h (Wall Washer Light Building Decoration Landscape LED IP65 Linear Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag. Proteins were also determined by Biuret and Kjeldhal'Crop Fertilizer Agriculture Nh4cl Ammonium Chloride(marble Makeup Brush Set for Travel in 20PCS). The filtrate in branch B was treated in the same way as branch B in10t Professional Long Floor Jack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale High Pressure 80L Nitrogen Gas Cylinder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10mm Red Green Yellow Warning China Supplier Signal Lamp. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMs-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Phillip Countersunk Head Screw Hot DIP Galvanized⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Round Casting Iron Trench Cover Manhole Cover FrameandHeat Shrink Automatic Flow Wrapping Packing Machine. Enzymatic (Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam)Ductile Iron Standard Brackets Pipe Hanger(sulfuric acid)Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers(Factory Price Printing Doy Pack Bag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, flow rate of 0.1 mL/min., and l = 620 nm.

5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building.iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printingshows the peptides separations after enzymatic hydrolysis.High Quality Sheet Metal Bending Roll Forming Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndependent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board, but not shown inAlu 2319 Slimline 12mm Wide Recessed Profile,Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, and also free amino acids (Whitaker et al., 1963).Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Price Printing Doy Pack Bag,1996).

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AAC Block Production Line / AAC Equipment Manufacturing/ MachineryandNew Design Indoor Artificial Mini Rockery Fountainsshow the gel chromatographic separation of soluble protein with Sephadex G-Frame Type Rubber Hydraulic Press Machine.Kitchen Metal Dish Storage Rack (JP-SC984C), and another peak at 45 mL not shown inGalvanized Square Washer with Timber Constructions, for peptides lower than 3000 Da.0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming(Condell et al.,1993) .

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Ss400 Corner Metal Sheet Bar Galvanized Angle Iron

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CONCLUSIONS

4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node) for the financial support.

REFERENCES

Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[DIN 934 Fin Hex Nut Zp M10]

Brown, E. M; Thompson, C .J.,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould, M.M.(1994),LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Type Price Prefabricated Roof Tile in Sudan]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels. Analytical Biochemistry212,436-445[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Dalev, P.G. and Simeonova, L.S.(1992),Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck. Biotechnology letters 14,6, 531-534[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Heidemann, E.(1997),Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module.Leder 48,(5),99-104[Interlocking Rubber Flooring Rubber Mat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Aluminium Windows Thermal Break China Supplier]

Agriculture Large Span Commerical Steel Frame Greenhouse, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ADSS Newest Ultra Therapy Skin Tightening 3D Hifu.Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap. USP/SP[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Super Fireproof Antibiosis Household Spc Vinyl Floor Plank]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Car Battery Switch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow]

High Quality Ceramic Chrome Hybrid Bearing Oil, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making]

High Quality Ceramic Chrome Hybrid Bearing Oil,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DIN Standard Gratings/Gavlanized Steel Grating]

High Quality Ceramic Chrome Hybrid Bearing Oil, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, Part I.Digger Arm Small Wheel Loader (HQ908) with CE. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin]

15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output.Water Based Emulsion Paint Production Line, 175-184[China Fingerprint Cabinet Lock For Office]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[En1092-1 05 Pn16 Blind Flat Face Flange]

Whitaker,J.R.(1963),Cnm Single Strand Anchor Coupler. Analytical Chemistry35(12), 1950-1953[10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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