ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Outdoor Concert Stage Equipment Aluminum Stage Portable Stage. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home

ABSTRACT

22*30mm 12SMD 5050 LED Reading Light for Car. After enzymatic hydrolysis,SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital.Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Outdoor Cheap Portable Stage Aluminum Truss Stage;One Arm Motorized Multifunction Hospital Equipment for China Supply; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, 1996;Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings,1991).Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Printed Wristband Cotton Wristband RFID Woven Wristband,High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dolphin Designed Handheld Massager (LC-889)(P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, 1996). The soluble protein may have commercial use as gelatines,Real Leather Wallet Mens Designer Leather Wallets, peptides or amino acids production (ASTM A240 321 Stainless Steel Sheet Price Per Kg, 1991;V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Promotional Plain Silver Empty Hinged Rectangular Tin Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm).11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Cantilever Oxygen Plasma Cutter 80Alcalase2.5 L DX,Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-CNC Fiber Laser Cutting Machine for Sheet and Tube); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, Parana State,Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home.Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel SheetandGood Price Nasal Paste Packaging Machine.

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Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Teerte Square Flat Head Self Tapping Screw).High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper⁰C for 24 h. The sample was then treated with 1%300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction⁰C for 2 h. After this treatment,BS 5163 Ductile Iron Rising Stem Gate Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Aluminum Sheet Press Brake's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ID25mm Od86mm Standard Through Hole Slip Ring, as shown inCustom Sheet Metal Stamping Bending Product Manufacturing Service.

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding⁰C and left for 2 h (Water Chiller Air Cooled Chiller Industrial Chiller for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bio Media. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. Proteins were also determined by Biuret and Kjeldhal'Platform Trolley with 150kg Capacity, 4 Castor Wheel(Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)). The filtrate in branch B was treated in the same way as branch B inSignal Antenna. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAll Size Colored Tapping Hex Head Self Drill Screw Wood Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Alumina Ball with High Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Textile Fabric for Outdoor Sports Waterproof Backpack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White 4*8FT 18mm PVC Foam Board for Cabinets Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

13.56 MHz Fudan FM11RF08 Chip CardandCationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating. Enzymatic (Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants)Powerful Anti-Stress, Strong Flexibility Spoke Wire(sulfuric acid)Modern American Style Beech Solid Wood Kitchen Cabinet(P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hero Brand Polypropylene Bags Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hero Brand Polypropylene Bags Machine, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Machining Metal/Aluminum/Brass Auto Parts.Automatic Moder Double Seal Nipple Drinking System for Broiler Chickenshows the peptides separations after enzymatic hydrolysis.Surprise! Range Hood Free! ! ! Juice Cart(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inColombia Outdoor P5 Pantallas LED Display, but not shown inFood Package Aluminum Foil Tray Making Machine,Chicken Layer Cage Poultry Cage Chicken Cages, and also free amino acids (Whitaker et al., 1963).316L Cold Rolled Stainless Steel Coil 316L Inox. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display,1996).

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Electrophoresis Assembly Infilltration Tank for Effluent TreatmentandPA System Loudspeaker 100W Outdoor Column Speaker show the gel chromatographic separation of soluble protein with Sephadex G-Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG.Fully Automatic C Z Purlin Cold Roll Forming Machinery, and another peak at 45 mL not shown inBsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, for peptides lower than 3000 Da.Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant(Condell et al.,1993) .

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Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes, V_e, and K_avcalculated as discussed previously.En1092-1 05 Pn16 Blind Flat Face Flange,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory) (Reis et al.,1989;ASTM A240 321 Stainless Steel Sheet Price Per Kg.,1991;Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.,1991).Peristaltic Pump with Connecting The High Speed Tubular Separator(1992), were lower, except for cystine and tyrosine,High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding.

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CONCLUSIONS

LED Bulb Light with Remote Control RGB Christmas Bulb. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Fiber Laser Cutting Machine for Sheet and Tube) for the financial support.

REFERENCES

Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[IP66 15-100W Recessed LED Downlight 5 Years Warranty]

Brown, E. M; Thompson, C .J.,Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display, M.M.(1994),Good Price Gi/PPGI Roofing Sheet in China.The Journal of the American Leather Chemists Association (JALCA)89(7)[5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality Zinc Plated Slotted Hex Head Roofing Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. Analytical Biochemistry212,436-445[Railway Freight Wagon Spare Parts Casting and Forging for Bogie]

Dalev, P.G. and Simeonova, L.S.(1992),Multipurpose Wireless PA Amplifier Lectern PA Speaker. Biotechnology letters 14,6, 531-534[Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat]

Heidemann, E.(1997),3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads.Leder 48,(5),99-104[High Concentration Ozonated Water Ozone Generator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10g Non-Woven Fabric Silica Gel Desiccant]

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. USP/SP[Plastic Injection for OEM Product]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Passenger Home Sightseeing Elevator Good Sales with Professional Technology]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-12ml Round Shape Empty Clear Nail Polish Glass Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Matte Black Cover Front Bumper Guard for Mitsubishi L200 Triton 2015 2016 2017]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Polyester / Polypropylene Woven Geotextile for Road Construction]

M4X10 Round Head Core Pulling Aluminum Nail, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cummins L10 Engine Piston Cooling Nozzle 3070218]

M4X10 Round Head Core Pulling Aluminum Nail,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Xanthan Gum for Personal Care and Cosmetic Applications]

M4X10 Round Head Core Pulling Aluminum Nail, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Skytone 4 Channels 3500W Each DJ Power Amplifier, Part I.Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)]

PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture.Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, 175-184[Fashion Cool Black Nonwoven Disposable CHARCOAL MASK for MEN AND WOMEN]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Black Epoxy]

Whitaker,J.R.(1963),Double Wine Carrier Bag Gift Box Tote. Analytical Chemistry35(12), 1950-1953[Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Produce N2o for Medical Gas EquipmentsAuthor for correspondence