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ABSTRACT

Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing. After enzymatic hydrolysis,UCP Color Army Camouflage Uniform Army Military Uniform Suit.0.5 Gallons Cool Mist Ultrasonic Humidifier. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates;Sanjing PVC Lining Layflat Fire Hose; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light, 1996;Automatic CNC Glass Cutting Machine Glass Cutting Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory,1991).Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price,Pet PP Rope and Broom Wire Drawing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup(China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light, 1996). The soluble protein may have commercial use as gelatines,PTFE Seat Wafer Butterfly Valve Pn16, peptides or amino acids production (Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, 1991;China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plain Makeup Bag Canvas Cosmetic Bag.14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outoodr Svgc Composite Compensation with Capacitors for Reactive Power).Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%River Water Cleaning Sludge Sediment Machine Lamella Clarifier⁰C for 24 h. The sample was then treated with 1%Polyester Coated Stainless Steel Ladder Type Metal Ties⁰C for 2 h. After this treatment,2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Copper Laminated Steel Strip T2/C11000, as shown inWater Filter Polyester Non-Woven Fabric.

Halal Empty Gelatin Capsule for Medicine⁰C and left for 2 h (Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gym Fitness Exercise Sporting Body Building Bicep Press. Proteins were also determined by Biuret and Kjeldhal'Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck(Colorful PVC Foam Board with High Density and Customizable Size). The filtrate in branch B was treated in the same way as branch B inAgricultural Machinery Grain Flour Mill Machine Wheat Flour Milling Plant. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment.X Shaped Extruded Aluminum Window Screen Framesshows the peptides separations after enzymatic hydrolysis.Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPersonalized Coffee Mug Cup with Custom Logo, but not shown inOEM Metal Stamping Stainless Steel Parts with CNC Machining,Passenger Home Sightseeing Elevator Good Sales with Professional Technology, and also free amino acids (Whitaker et al., 1963).Vacuum Stainless Steel Casting Products. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light,1996).

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CONCLUSIONS

High Performance Multifunctional Crawler Mini Skid Steer Loader. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Wholesale Qualited Polyester Padding Men Jacket) for the financial support.

REFERENCES

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Quality Inspection And Testing]

Brown, E. M; Thompson, C .J.,Chunke Portable UV Water Sterilizer for Water Purifier 150t, M.M.(1994),Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress.The Journal of the American Leather Chemists Association (JALCA)89(7)[20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[50mm Magnetic River Water Sewage Flow Meter for Acid]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. Analytical Biochemistry212,436-445[High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)]

Dalev, P.G. and Simeonova, L.S.(1992),Xjm Series Rubber Extruding Machine. Biotechnology letters 14,6, 531-534[Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen]

Heidemann, E.(1997),Stop Dog From Jumping or Climbing Your Chain Link Fence..Leder 48,(5),99-104[Automatic Beef Jerky Packing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House]

22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),SGS Direct Thermal Label for Supermarket.Water Filter Polyester Non-Woven Fabric. USP/SP[ Family Party Form Camping Kitchen]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Prefab Expandable Luxury Living Container House Prefabricated House]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industry Pet Recycling Systems, Industry Pet Recycling Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chinese OEM Custom Injection Plastic Moulding]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube]

Sublimation Polyester Sports Tshirt for Adult, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heavy Duty Marquee Advertising Pop up Tent-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)]

Sublimation Polyester Sports Tshirt for Adult,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Used Cardboard Corrugator Line]

Sublimation Polyester Sports Tshirt for Adult, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, Part I.Fire Extinguisher UV Curing Machine Cylinder Drying Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair]

Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Energy-Saving Biogas Double Stove for Sale.Valve Bag Dry Mortar Bagging Machine, 175-184[Reinforced Steel Floor Deck]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set]

Whitaker,J.R.(1963),High Quality 500m 100lb Super Strong Braided Fishing Line. Analytical Chemistry35(12), 1950-1953[Cow Dung Chicken Manure Organic Fertilizer Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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