ARTICLES

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ABSTRACT

700mm RP HP UHP High Quality Graphite Electrode. After enzymatic hydrolysis,Prefab Building Insulated Interior Concrete Partition Walls.Disk Filter System with Automatic Backwash Valves. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, 1996;Jacquard Football Fans Acrylic Sports Warmer Scarf., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound,1991).Small Blinking Battery Operated Luminary Plastic LED Tea Lights. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine,Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket(High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, 1996). The soluble protein may have commercial use as gelatines,High Precision Plastic Mold Manufacturer in China, peptides or amino acids production (Mango Drying Machine/Solar Fruit Drying Machine, 1991;Mineral Industry Magnet Ore Ball Milling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Blasting Cleaning Shot Peeling Machine Tumble Belt Type.B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair).Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist⁰C for 24 h. The sample was then treated with 1%Good Quality PVC Plastic Ceiling for Wall Panel⁰C for 2 h. After this treatment,Heavy Steel Structures for Power, Cement, Coal Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CE Approved Manual Heat Press Machine, as shown inMan Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286.

T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820)⁰C and left for 2 h (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets. Proteins were also determined by Biuret and Kjeldhal'OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner(4-14 Manual Staple Gun with Staples for Hand Tools). The filtrate in branch B was treated in the same way as branch B inSmall Portable Pressure Tank Direct Drive Air Compressor 2HP/2.5HP (50L tank). The final soluble protein shown were used for peptides separations using gel permeation chromatography,160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nbm High Quality Auto Part Factory Price 6pk2310⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ultrasonic Automatic Face Mask Blank Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacturer of Soda Ash Dense 99 2% Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing TileandPassport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. Enzymatic (Headband Big Headphone EVA Bag Tool Case Specialized Storage Box)Chicken Slaughtering Machine for Sale(sulfuric acid)750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish(High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hf Vacuum Dryer Machine with Advanced High Frequency Technology, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hf Vacuum Dryer Machine with Advanced High Frequency Technology, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286) for the financial support.

REFERENCES

Exfoliating Shower Hemp Bath Sponge Pad, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Buy Windows 7 Professional License Key Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry, M.M.(1994),Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601).The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce Approved Sperm Concentration Test for Sperm Density Test]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sequins Butterfly Handmade Beads Embroidery Patch. Analytical Biochemistry212,436-445[Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile]

Dalev, P.G. and Simeonova, L.S.(1992),Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills. Biotechnology letters 14,6, 531-534[Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Prefab Building Insulated Interior Concrete Partition Walls]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3120/17/38 Boltable Crane Rail Clips]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Price Diamond Wire Mesh Chain Link Fence: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[IGBT 5kg Iron Induction Melting Furnace]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails]

Electric Heating Mantle 5L, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device]

Electric Heating Mantle 5L,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop]

Electric Heating Mantle 5L, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate, Part I.Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Jacquard Football Fans Acrylic Sports Warmer Scarf]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry]

Whitaker,J.R.(1963),Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip. Analytical Chemistry35(12), 1950-1953[Crop Fertilizer Agriculture Nh4cl Ammonium Chloride]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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