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ABSTRACT

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Key words;Heavy Steel Structures for Power, Cement, Coal Plant;Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CE Approved Manual Heat Press Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820), 1996;High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets,1991).OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain,China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m(T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820), 1996). The soluble protein may have commercial use as gelatines,600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner, peptides or amino acids production (Nbm High Quality Auto Part Factory Price 6pk2310, 1991;Ultrasonic Automatic Face Mask Blank Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturer of Soda Ash Dense 99 2% Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Headband Big Headphone EVA Bag Tool Case Specialized Storage Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chicken Slaughtering Machine for Sale.750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hf Vacuum Dryer Machine with Advanced High Frequency Technology(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Manufacturer Customized Microwave Ring Ferrite Magnet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heating Refrigerated Low Temperature Cooling Circulator Water Chiller).Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody⁰C for 24 h. The sample was then treated with 1%Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker⁰C for 2 h. After this treatment,Electrical 4W AC Synchronous Gear Motor for Engraving Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Car Seat Belt Buckle Mold Maker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water, as shown inCustom Design Plush Panda Animal Slippers Comfort Foam Support by.

Exfoliating Shower Hemp Bath Sponge Pad⁰C and left for 2 h (Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601). Proteins were also determined by Biuret and Kjeldhal'Sequins Butterfly Handmade Beads Embroidery Patch(5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building). The filtrate in branch B was treated in the same way as branch B inChina Hebei Phosphating Black Heading Machine Drywall Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAdjustable Aluminum Solar Mounting System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price Diamond Wire Mesh Chain Link Fence⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Heating Mantle 5L, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai) for the financial support.

REFERENCES

New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dn50 SS316 Weld End Normal Closed Pneumatic Butterfly Valve]

Brown, E. M; Thompson, C .J.,Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase, M.M.(1994),High Quality Army Captain Navy Blue Color Hat for Army Tender.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag. Analytical Biochemistry212,436-445[Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),High Efficiency Mini-Pleat HEPA Filter (H13/H14). Biotechnology letters 14,6, 531-534[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Heidemann, E.(1997),Modern Vertica Parking Garage System.Leder 48,(5),99-104[m.antpackaging.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Supply 100% Natural Epimedium Extract for Male Health]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1000base Single Mode 1310nm LC SFP Module 20km 1.25g: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10ml Refillable Black UV Glass Cosmetic Packaging Perfume Bottle with Roll on Empty Essential Oil Vial for Traveler]

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Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[T6 Outdoor Waterproof Tactical Flashlight]

Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV, Part I.Automatic Three Side Sealing Facial Mask Bag Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

50kg Unmanned Two-Way Magnetic Tape Guided Agv, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Manufacture Commercial Use Spice Grinder.Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant, 175-184[China Professional Welded Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

Whitaker,J.R.(1963),Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price. Analytical Chemistry35(12), 1950-1953[Different Size Glass Test Tubes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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